Restriction map for pF1KB8890
 (Circular) (Six-base) MAPSORT of: pF1KB8890.seq  Check: 9642  from: 1  to: 3737



pF1KB8890  3737 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AccI GT'mk_AC

Cuts at:    775     775 
   Size:       3737
 
Acc65I G'GTAC_C

Cuts at:    753     753 
   Size:       3737
 
AclI AA'CG_TT

Cuts at:   3577    3577 
   Size:       3737
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1562    1994    2741    3009    1562 
   Size:        432     747     268    2290

  Fragments arranged by size: 

                2290     747     432     268
 
AflII C'TTAA_G

Cuts at:    286     286 
   Size:       3737
 
AflIII A'CryG_T

Cuts at:   1089    2193    3170    1089 
   Size:       1104     977    1656

  Fragments arranged by size: 

                1656    1104     977
 
AgeI A'CCGG_T

Cuts at:   2125    2125 
   Size:       3737
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    580     580 
   Size:       3737
 
AlwNI CAG_nnn'CTG

Cuts at:   1042    2609    1042 
   Size:       1567    2170
 
ApaI G_GGCC'C

Cuts at:    324     324 
   Size:       3737
 
ApaLI G'TGCA_C

Cuts at:   2507    2507 
   Size:       3737
 
ApoI r'AATT_y

Cuts at:    741    3090    3346     741 
   Size:       2349     256    1132

  Fragments arranged by size: 

                2349    1132     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3737
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3737
 
AvaI C'yCGr_G

Cuts at:    757     757 
   Size:       3737
 
BamHI G'GATC_C

Cuts at:    762     762 
   Size:       3737
 
BanI G'GyrC_C

Cuts at:    114     148     348     564     753    1167    1415    1450     114 
   Size:         34     200     216     189     414     248      35    2401

  Fragments arranged by size: 

                2401     414     248     216     200     189      35      34
            
 
BanII G_rGCy'C

Cuts at:    324     751    1781     324 
   Size:        427    1030    2280

  Fragments arranged by size: 

                2280    1030     427
 
BbeI G_GCGC'C

Cuts at:   1171    1419    1171 
   Size:        248    3489
 
BciVI GTATCCnnnnn_n'

Cuts at:   1635    2396    3737    1635 
   Size:        761    1341    1635

  Fragments arranged by size: 

                1635    1341     761
 
BclI T'GATC_A

Cuts at:   1257    1257 
   Size:       3737
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       3737
 
BglII A'GATC_T

Cuts at:   1252    3289    1252 
   Size:       2037    1700
 
BlpI GC'TnA_GC

Cuts at:    849     849 
   Size:       3737
 
Bme1580I G_kGCm'C

Cuts at:    151     324     351    1362    1455    2511     151 
   Size:        173      27    1011      93    1056    1377

  Fragments arranged by size: 

                1377    1056    1011     173      93      27
 
BmrI ACTGGGnnnn_n'

Cuts at:    152     949    1030    1365    3509     152 
   Size:        797      81     335    2144     380

  Fragments arranged by size: 

                2144     797     380     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2104    2104 
   Size:       3737
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    104     104 
   Size:       3737
 
Bpu10I CC'TnA_GC

Cuts at:    500     500 
   Size:       3737
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    913    1792    2284    2582     913 
   Size:        879     492     298    2068

  Fragments arranged by size: 

                2068     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    657    3441     657 
   Size:       2784     953
 
BsaAI yAC'GTr

Cuts at:   1090    1090 
   Size:       3737
 
BsaBI GATnn'nnATC

Cuts at:      6    1251    3288       6 
   Size:       1245    2037     455

  Fragments arranged by size: 

                2037    1245     455
 
BsaHI Gr'CG_yC

Cuts at:   1168    1416    2118    3614    1168 
   Size:        248     702    1496    1291

  Fragments arranged by size: 

                1496    1291     702     248
 
BsaWI w'CCGG_w

Cuts at:    926    1447    2125    2399    2546    2957     926 
   Size:        521     678     274     147     411    1706

  Fragments arranged by size: 

                1706     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    157     187     157 
   Size:         30    3707
 
BseYI C'CCAG_C

Cuts at:    637    1162    1920    2497    2993     637 
   Size:        525     758     577     496    1381

  Fragments arranged by size: 

                1381     758     577     525     496
 
BsiEI CG_ry'CG

Cuts at:    104    1279    1325    2533     104 
   Size:       1175      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1175      46
 
BsiHKAI G_wGCw'C

Cuts at:    751    1529    1719    2511     751 
   Size:        778     190     792    1977

  Fragments arranged by size: 

                1977     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    151     324     351     751    1362    1455    1529    1719    1781 
   Size:        173      27     400     611      93      74     190      62

Cuts at:   1781    2511     151 
   Size:        730    1377

  Fragments arranged by size: 

                1377     730     611     400     190     173      93      74
                  62      27
 
BspEI T'CCGG_A

Cuts at:   2957    2957 
   Size:       3737
 
BspHI T'CATG_A

Cuts at:    527    2913     527 
   Size:       2386    1351
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    787    1001    1303    1684     787 
   Size:        214     302     381    2840

  Fragments arranged by size: 

                2840     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2029    2083    3567    3732      50 
   Size:       1979      54    1484     165      55

  Fragments arranged by size: 

                1979    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    428    1649     428 
   Size:       1221    2516
 
BsrFI r'CCGG_y

Cuts at:   1735    1916    2125    1735 
   Size:        181     209    3347

  Fragments arranged by size: 

                3347     209     181
 
BsrGI T'GTAC_A

Cuts at:    542     542 
   Size:       3737
 
BssSI C'ACGA_G

Cuts at:    610    2008    2366     610 
   Size:       1398     358    1981

  Fragments arranged by size: 

                1981    1398     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1114    1114 
   Size:       3737
 
BstBI TT'CG_AA

Cuts at:   2098    2098 
   Size:       3737
 
BstYI r'GATC_y

Cuts at:      1     762    1229    1252    1587    1833    2845    2931    2943 
   Size:        761     467      23     335     246    1012      86      12

Cuts at:   2943    3289       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     761     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3737
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1687    1868    2031    1687 
   Size:        181     163    3393

  Fragments arranged by size: 

                3393     181     163
 
BtsI GCAGTG_nn'

Cuts at:    980     980 
   Size:       3737
 
DraI TTT'AAA

Cuts at:    736     736 
   Size:       3737
 
DrdI GACnn_nn'nnGTC

Cuts at:   1443    2301    1443 
   Size:        858    2879
 
EaeI y'GGCC_r

Cuts at:   1322    1496    1887    1914    3259    1322 
   Size:        174     391      27    1345    1800

  Fragments arranged by size: 

                1800    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1322    1322 
   Size:       3737
 
EarI CTCTTCn'nnn_

Cuts at:   1760    1970    1760 
   Size:        210    3527
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1945    2255    2401    3547    1945 
   Size:        310     146    1146    2135

  Fragments arranged by size: 

                2135    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    749     749 
   Size:       3737
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    104    1562    1994    2741    3009     104 
   Size:       1458     432     747     268     832

  Fragments arranged by size: 

                1458     832     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    175     286     175 
   Size:        111    3626
 
EcoO109I rG'GnC_Cy

Cuts at:    320     876     320 
   Size:        556    3181
 
EcoRI G'AATT_C

Cuts at:    741     741 
   Size:       3737
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1756    1756 
   Size:       3737
 
FspI TGC'GCA

Cuts at:   1518    1518 
   Size:       3737
 
HaeII r_GCGC'y

Cuts at:   1171    1419    2441    3420    1171 
   Size:        248    1022     979    1488

  Fragments arranged by size: 

                1488    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    580     612     637     669    1579    1609    1611    1641     580 
   Size:         32      25      32     910      30       2      30    2676

  Fragments arranged by size: 

                2676     910      32      32      30      30      25       2
            
 
HincII GTy'rAC

Cuts at:    776     776 
   Size:       3737
 
KasI G'GCGC_C

Cuts at:   1167    1415    1167 
   Size:        248    3489
 
KpnI G_GTAC'C

Cuts at:    757     757 
   Size:       3737
 
MluI A'CGCG_T

Cuts at:   3170    3170 
   Size:       3737
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2408    2592    2408 
   Size:        184    3553
 
MscI TGG'CCA

Cuts at:   1498    1498 
   Size:       3737
 
MslI CAynn'nnrTG

Cuts at:    130     157     562    1726    1853     130 
   Size:         27     405    1164     127    2014

  Fragments arranged by size: 

                2014    1164     405     127      27
 
MspA1I CmG'CkG

Cuts at:    637     848    1162    1522    2535    2780     637 
   Size:        211     314     360    1013     245    1594

  Fragments arranged by size: 

                1594    1013     360     314     245     211
 
NaeI GCC'GGC

Cuts at:   1918    1918 
   Size:       3737
 
NarI GG'CG_CC

Cuts at:   1168    1416    1168 
   Size:        248    3489
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3737
 
NgoMIV G'CCGG_C

Cuts at:   1916    1916 
   Size:       3737
 
NspI r_CATG'y

Cuts at:    790    1290    2197     790 
   Size:        500     907    2330

  Fragments arranged by size: 

                2330     907     500
 
PciI A'CATG_T

Cuts at:   2193    2193 
   Size:       3737
 
PflMI CCAn_nnn'nTGG

Cuts at:    273     408     273 
   Size:        135    3602
 
PmeI GTTT'AAAC

Cuts at:    736     736 
   Size:       3737
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2876    2908    2876 
   Size:         32    3705
 
PspOMI G'GGCC_C

Cuts at:    320     320 
   Size:       3737
 
PstI C_TGCA'G

Cuts at:    784    1469     784 
   Size:        685    3052
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3737
 
PvuII CAG'CTG

Cuts at:   1162    1522    1162 
   Size:        360    3377
 
RsrII CG'GwC_CG

Cuts at:   1932    1932 
   Size:       3737
 
SacI G_AGCT'C

Cuts at:    751     751 
   Size:       3737
 
SalI G'TCGA_C

Cuts at:    774     774 
   Size:       3737
 
SapI GCTCTTCn'nnn_

Cuts at:   1760    1970    1760 
   Size:        210    3527
 
SbfI CC_TGCA'GG

Cuts at:    784     784 
   Size:       3737
 
ScaI AGT'ACT

Cuts at:   3254    3254 
   Size:       3737
 
SfcI C'TryA_G

Cuts at:     33     780    1465    2458    2649      33 
   Size:        747     685     993     191    1121

  Fragments arranged by size: 

                1121     993     747     685     191
 
SfoI GGC'GCC

Cuts at:   1169    1417    1169 
   Size:        248    3489
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3737
 
SmaI CCC'GGG

Cuts at:    759     759 
   Size:       3737
 
SmlI C'TyrA_G

Cuts at:    286     892    1807    2299    2561     286 
   Size:        606     915     492     262    1462

  Fragments arranged by size: 

                1462     915     606     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3737
 
SphI G_CATG'C

Cuts at:    790    1290     790 
   Size:        500    3237
 
StuI AGG'CCT

Cuts at:    592     592 
   Size:       3737
 
StyI C'CwwG_G

Cuts at:    107     871    1225     107 
   Size:        764     354    2619

  Fragments arranged by size: 

                2619     764     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1320    1453    1912    2121    1320 
   Size:        133     459     209    2936

  Fragments arranged by size: 

                2936     459     209     133
 
TatI w'GTAC_w

Cuts at:    542    3252     542 
   Size:       2710    1027
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3254    3286    3254 
   Size:         32    3705
 
Tth111I GACn'n_nGTC

Cuts at:    649    1534     649 
   Size:        885    2852
 
XbaI T'CTAG_A

Cuts at:    768     768 
   Size:       3737
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    159     223     726    1232     159 
   Size:         64     503     506    2664

  Fragments arranged by size: 

                2664     506     503      64
 
XmaI C'CCGG_G

Cuts at:    757     757 
   Size:       3737
 
XmnI GAAnn'nnTTC

Cuts at:    670     670 
   Size:       3737
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AloI     AscI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI     BmtI    BseRI
     BsgI    BsiWI     BsmI    BsmBI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com