Restriction map for pF1KB8866
##################################
# NO CUT ENZYMES (  193 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       HphI        KasI        
KpnI        KspI        MaeII       MaeIII      MamI        
MfeI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tsp45I      
TspRI       Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Alw44I        184  GTGCAC               G^TGCAC
ApaLI         184  GTGCAC               G^TGCAC
AspI          259  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII         206  GAGCTC               G[GA]GC[CT]^C
BbsI          256  GAAGAC               GAAGAC(2/6)
BfaI          214  CTAG                 C^TAG
BglII          91  AGATCT               A^GATCT
BpuAI         256  GAAGAC               GAAGAC(2/6)
BseRI         209  CTCCTC               GAGGAG(10/8)
BsiYI         246  CCGAAAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          246  CCGAAAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI           6  ACCTGC               ACCTGC(4/8)
BssKI         123  CCTGG                ^CC[ACGT]GG
BstAPI        177  GCAGGATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         123  CCTGG                CC^[AT]GG
BstYI          91  AGATCT               [GA]^GATC[CT]
Cfr13I        242  GGCCC                G^G[ACGT]CC
DpnII          92  GATC                 ^GATC
DraII         241  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EarI           87  GAAGAG               CTCTTC(1/4)
EcoO109I      241  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRII        123  CCTGG                ^CC[AT]GG
EcoT14I       201  CCTTGG               C^C[AT][AT]GG
EcoT22I       115  ATGCAT               ATGCA^T
HaeIII        242  GGCC                 GG^CC
Ksp632I        87  GAAGAG               CTCTTC(1/4)
MaeI          214  CTAG                 C^TAG
MboI           92  GATC                 ^GATC
MflI           91  AGATCT               [GA]^GATC[CT]
MvaI          123  CCTGG                CC^[AT]GG
NdeII          92  GATC                 ^GATC
NsiI          115  ATGCAT               ATGCA^T
PflFI         259  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          175  CTGCAG               CTGCA^G
SacI          206  GAGCTC               GAGCT^C
Sau3AI         92  GATC                 ^GATC
Sau96I        242  GGCCC                G^G[ACGT]CC
ScrFI         123  CCTGG                CC^[ACGT]GG
SfaNI         226  GATGC                GCATC(5/9)
SfcI          175  CTGCAG               C^T[GA][CT]AG
StyI          201  CCTTGG               C^C[AT][AT]GG
TaqI          142  TCGA                 T^CGA
Tth111I       259  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       142  TCGA                 T^CGA
XhoII          91  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AspHI         184  GTGCAC               G[AT]GC[AT]^C
              206  GAGCTC              
BbvI          162  GCTGC                GCAGC(8/12)
              174  GCTGC               
BmyI          184  GTGCAC               G[AGT]GC[ACT]^C
              206  GAGCTC              
BsaJI         123  CCTGGG               C^C[ACGT][ACGT]GG
              201  CCTTGG              
BsiHKAI       184  GTGCAC               G[AT]GC[AT]^C
              206  GAGCTC              
Bsp1286I      184  GTGCAC               G[AGT]GC[ACT]^C
              206  GAGCTC              
BstF5I         69  GGATG                GGATG(2/0)
              180  GGATG               
Fnu4HI        162  GCTGC                GC^[ACGT]GC
              174  GCTGC               
FokI           69  GGATG                GGATG(9/13)
              180  GGATG               
ItaI          162  GCTGC                GC^[ACGT]GC
              174  GCTGC               
MboII          87  GAAGA                GAAGA(8/7)
              256  GAAGA               
MseI           83  TTAA                 T^TAA
              325  TTAA                
NlaIV         232  GGTTCC               GG[ACGT]^[ACGT]CC
              242  GGCCCC              
Tru9I          83  TTAA                 T^TAA
              325  TTAA                
TseI          162  GCTGC                G^C[AT]GC
              174  GCTGC               
Tsp509I        48  AATT                 ^AATT
              190  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

AluI        MnlI        MwoI        NlaIII      
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