##################################
# NO CUT ENZYMES (  213 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       ItaI        KasI        KpnI        
KspI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        TseI        Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI          132  AAATTC               [GA]^AATT[CT]
AfaI          121  GTAC                 GT^AC
ApoI          132  AAATTC               [GA]^AATT[CT]
BsiYI         244  CCTACAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          244  CCTACAGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         326  GTCTC                GTCTC(1/5)
BsmI          287  GCATTC               GAATGC(1/-1)
BsrI          299  CCAGT                ACTGG(1/-1)
BssKI         160  CCAGG                ^CC[ACGT]GG
BstNI         160  CCAGG                CC^[AT]GG
BstXI           6  CCAGCGTTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI          197  CTCAG                C^T[ACGT]AG
DpnII         174  GATC                 ^GATC
DraI           18  TTTAAA               TTT^AAA
DsaI          179  CCACGG               C^C[GA][CT]GG
EarI           85  GAAGAG               CTCTTC(1/4)
EcoNI         244  CCTACAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        160  CCAGG                ^CC[AT]GG
EcoT14I        80  CCTTGG               C^C[AT][AT]GG
HaeIII        258  GGCC                 GG^CC
HinfI         263  GACTC                G^A[ACGT]TC
HphI          176  TCACC                GGTGA(8/7)
Ksp632I        85  GAAGAG               CTCTTC(1/4)
MboI          174  GATC                 ^GATC
MseI           19  TTAA                 T^TAA
MvaI          160  CCAGG                CC^[AT]GG
NdeII         174  GATC                 ^GATC
NlaIII        193  CATG                 CATG^
PleI          263  GACTC                GAGTC(4/5)
RsaI          121  GTAC                 GT^AC
Sau3AI        174  GATC                 ^GATC
ScrFI         160  CCAGG                CC^[ACGT]GG
SfcI          245  CTACAG               C^T[GA][CT]AG
StyI           80  CCTTGG               C^C[AT][AT]GG
Tru9I          19  TTAA                 T^TAA
Tsp509I       133  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BfaI           59  CTAG                 C^TAG
              118  CTAG                
BsaJI          80  CCTTGG               C^C[ACGT][ACGT]GG
              179  CCACGG              
BstF5I         73  CATCC                GGATG(2/0)
              147  GGATG               
Cac8I           5  GCCAGC               GC[ACGT]^[ACGT]GC
              239  GCCAGC              
FokI           73  CATCC                GGATG(9/13)
              147  GGATG               
MaeI           59  CTAG                 C^TAG
              118  CTAG                
MboII          85  GAAGA                GAAGA(8/7)
              333  TCTTC               
MnlI           88  GAGG                 CCTC(7/6)
              251  GAGG                


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

TspRI