Restriction map for pF1KB8861
##################################
# NO CUT ENZYMES (  187 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstNI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MfeI        MflI        MluI        MroI        MseI        
MslI        MspA1I      MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SbfI        ScrFI       SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AfaI          225  GTAC                 GT^AC
AspHI         208  GAGCTC               G[AT]GC[AT]^C
BalI          248  TGGCCA               TGG^CCA
BbvCI          96  CCTCAGC              CCTCAGC(-5/-2)
BbvI            4  GCAGC                GCAGC(8/12)
BfaI           25  CTAG                 C^TAG
BpmI          228  CTGGAG               CTGGAG(16/14)
BsaBI         190  GATGTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI           40  GAGACC               GGTCTC(1/5)
BseRI          28  GAGGAG               GAGGAG(10/8)
BsiHKAI       208  GAGCTC               G[AT]GC[AT]^C
BsiYI          65  CCTTTGCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           65  CCTTTGCAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          40  GAGAC                GTCTC(1/5)
BsrI          227  ACTGG                ACTGG(1/-1)
Cac8I         246  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr13I         81  GGGCC                G^G[ACGT]CC
DdeI           97  CTCAG                C^T[ACGT]AG
DpnII         282  GATC                 ^GATC
DsaI           44  CCGTGG               C^C[GA][CT]GG
EaeI          248  TGGCCA               [CT]^GGCC[GA]
EarI          180  GAAGAG               CTCTTC(1/4)
EcoNI          65  CCTTTGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       251  CCAAGG               C^C[AT][AT]GG
Fnu4HI          4  GCAGC                GC^[ACGT]GC
HapII          84  CCGG                 C^CGG
HgaI          101  GCGTC                GACGC(5/10)
HincII        103  GTCAAC               GT[CT]^[GA]AC
HindII        103  GTCAAC               GT[CT]^[GA]AC
HpaII          84  CCGG                 C^CGG
ItaI            4  GCAGC                GC^[ACGT]GC
Ksp632I       180  GAAGAG               CTCTTC(1/4)
MaeI           25  CTAG                 C^TAG
MamI          190  GATGTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          282  GATC                 ^GATC
MluNI         248  TGGCCA               TGG^CCA
MscI          248  TGGCCA               TGG^CCA
MspI           84  CCGG                 C^CGG
MwoI          138  GCCCCATCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         282  GATC                 ^GATC
PshAI          42  GACCGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          225  GTAC                 GT^AC
SacI          208  GAGCTC               GAGCT^C
SapI          180  GAAGAGC              GCTCTTC(1/4)
Sau3AI        282  GATC                 ^GATC
Sau96I         81  GGGCC                G^G[ACGT]CC
ScaI          224  AGTACT               AGT^ACT
StyI          251  CCAAGG               C^C[AT][AT]GG
TfiI          195  GAATC                G^A[AT]TC
TseI            4  GCAGC                G^C[AT]GC
Tsp45I         49  GTCAC                ^GT[GC]AC
TspRI          14  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI           35  CCATTGAGACCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BanII         159  GGGCTC               G[GA]GC[CT]^C
              208  GAGCTC              
BmyI          159  GGGCTC               G[AGT]GC[ACT]^C
              208  GAGCTC              
BsaJI          44  CCGTGG               C^C[ACGT][ACGT]GG
              251  CCAAGG              
Bsp1286I      159  GGGCTC               G[AGT]GC[ACT]^C
              208  GAGCTC              
BstEII         48  GGTCACC              G^GT[ACGT]ACC
              123  GGTTACC             
BstF5I        153  GGATG                GGATG(2/0)
              189  GGATG               
BstPI          48  GGTCACC              G^GT[ACGT]ACC
              123  GGTTACC             
EcoO65I        48  GGTCACC              G^GT[ACGT]ACC
              123  GGTTACC             
FokI          153  GGATG                GGATG(9/13)
              189  GGATG               
HaeIII         82  GGCC                 GG^CC
              249  GGCC                
HphI           50  TCACC                GGTGA(8/7)
               62  TCACC               
MaeIII         49  GTCAC                ^GT[ACGT]AC
              124  GTTAC               
PleI          202  GACTC                GAGTC(4/5)
               31  GAGTC               
SmlI          148  CTCAAG               C^T[CT][GA]AG
              211  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

AluI        HinfI       MboII       MnlI        
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