Restriction map for
pF1KB8861
(Circular) (Six-base) MAPSORT of: pF1KB8861.seq Check: 9363 from: 1 to: 3407
pF1KB8861 3407 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 445 445
Size: 3407
Acc65I G'GTAC_C
Cuts at: 423 423
Size: 3407
AclI AA'CG_TT
Cuts at: 3247 3247
Size: 3407
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 1232 1664 2411 2679 406
Size: 826 432 747 268 1134
Fragments arranged by size:
1134 826 747 432 268
AflIII A'CryG_T
Cuts at: 759 1863 2840 759
Size: 1104 977 1326
Fragments arranged by size:
1326 1104 977
AgeI A'CCGG_T
Cuts at: 1795 1795
Size: 3407
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 307 339 307
Size: 32 3375
AlwNI CAG_nnn'CTG
Cuts at: 712 2279 712
Size: 1567 1840
ApaLI G'TGCA_C
Cuts at: 2177 2177
Size: 3407
ApoI r'AATT_y
Cuts at: 411 2760 3016 411
Size: 2349 256 802
Fragments arranged by size:
2349 802 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3407
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3407
AvaI C'yCGr_G
Cuts at: 427 427
Size: 3407
BamHI G'GATC_C
Cuts at: 432 432
Size: 3407
BanI G'GyrC_C
Cuts at: 423 837 1085 1120 423
Size: 414 248 35 2710
Fragments arranged by size:
2710 414 248 35
BanII G_rGCy'C
Cuts at: 271 320 421 1451 271
Size: 49 101 1030 2227
Fragments arranged by size:
2227 1030 101 49
BbeI G_GCGC'C
Cuts at: 841 1089 841
Size: 248 3159
BbvCI CC'TCA_GC
Cuts at: 205 205
Size: 3407
BciVI GTATCCnnnnn_n'
Cuts at: 1305 2066 3407 1305
Size: 761 1341 1305
Fragments arranged by size:
1341 1305 761
BclI T'GATC_A
Cuts at: 927 927
Size: 3407
BglII A'GATC_T
Cuts at: 922 2959 922
Size: 2037 1370
BlpI GC'TnA_GC
Cuts at: 519 519
Size: 3407
Bme1580I G_kGCm'C
Cuts at: 1032 1125 2181 1032
Size: 93 1056 2258
Fragments arranged by size:
2258 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 619 700 1035 3179 619
Size: 81 335 2144 847
Fragments arranged by size:
2144 847 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1774 1774
Size: 3407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 357
Size: 3407
Bpu10I CC'TnA_GC
Cuts at: 205 205
Size: 3407
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 304 583 1462 1954 2252 241
Size: 63 279 879 492 298 1396
Fragments arranged by size:
1396 879 492 298 279 63
BsaI GGTCTCn'nnnn_
Cuts at: 142 3111 142
Size: 2969 438
BsaAI yAC'GTr
Cuts at: 760 760
Size: 3407
BsaBI GATnn'nnATC
Cuts at: 6 302 921 2958 6
Size: 296 619 2037 455
Fragments arranged by size:
2037 619 455 296
BsaHI Gr'CG_yC
Cuts at: 838 1086 1788 3284 838
Size: 248 702 1496 961
Fragments arranged by size:
1496 961 702 248
BsaWI w'CCGG_w
Cuts at: 596 1117 1795 2069 2216 2627 596
Size: 521 678 274 147 411 1376
Fragments arranged by size:
1376 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 307 337 307
Size: 30 3377
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 151
Size: 3407
BseYI C'CCAG_C
Cuts at: 236 832 1590 2167 2663 236
Size: 596 758 577 496 980
Fragments arranged by size:
980 758 596 577 496
BsiEI CG_ry'CG
Cuts at: 104 949 995 2203 104
Size: 845 46 1208 1308
Fragments arranged by size:
1308 1208 845 46
BsiHKAI G_wGCw'C
Cuts at: 320 421 1199 1389 2181 320
Size: 101 778 190 792 1546
Fragments arranged by size:
1546 792 778 190 101
Bsp1286I G_dGCh'C
Cuts at: 271 320 421 1032 1125 1199 1389 1451 2181
Size: 49 101 611 93 74 190 62 730
Cuts at: 2181 271
Size: 1497
Fragments arranged by size:
1497 730 611 190 101 93 74 62
49
BspEI T'CCGG_A
Cuts at: 2627 2627
Size: 3407
BspHI T'CATG_A
Cuts at: 2583 2583
Size: 3407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 457 671 973 1354 457
Size: 214 302 381 2510
Fragments arranged by size:
2510 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1699 1753 3237 3402 50
Size: 1649 54 1484 165 55
Fragments arranged by size:
1649 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1319 1319
Size: 3407
BsrFI r'CCGG_y
Cuts at: 1405 1586 1795 1405
Size: 181 209 3017
Fragments arranged by size:
3017 209 181
BssSI C'ACGA_G
Cuts at: 1678 2036 1678
Size: 358 3049
BstAPI GCAn_nnn'nTGC
Cuts at: 784 784
Size: 3407
BstBI TT'CG_AA
Cuts at: 1768 1768
Size: 3407
BstEII G'GTnAC_C
Cuts at: 156 231 156
Size: 75 3332
BstYI r'GATC_y
Cuts at: 1 432 899 922 1257 1503 2515 2601 2613
Size: 431 467 23 335 246 1012 86 12
Cuts at: 2613 2959 1
Size: 346 449
Fragments arranged by size:
1012 467 449 431 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 152 107
Size: 45 3362
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1357 1538 1701 1357
Size: 181 163 3063
Fragments arranged by size:
3063 181 163
BtsI GCAGTG_nn'
Cuts at: 650 650
Size: 3407
DraI TTT'AAA
Cuts at: 406 406
Size: 3407
DrdI GACnn_nn'nnGTC
Cuts at: 1113 1971 1113
Size: 858 2549
EaeI y'GGCC_r
Cuts at: 356 992 1166 1557 1584 2929 356
Size: 636 174 391 27 1345 834
Fragments arranged by size:
1345 834 636 391 174 27
EagI C'GGCC_G
Cuts at: 992 992
Size: 3407
EarI CTCTTCn'nnn_
Cuts at: 283 1430 1640 283
Size: 1147 210 2050
Fragments arranged by size:
2050 1147 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1615 1925 2071 3217 1615
Size: 310 146 1146 1805
Fragments arranged by size:
1805 1146 310 146
EcoICRI GAG'CTC
Cuts at: 318 419 318
Size: 101 3306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 357 406 1232 1664 2411 2679 357
Size: 49 826 432 747 268 1085
Fragments arranged by size:
1085 826 747 432 268 49
EcoNI CCTnn'n_nnAGG
Cuts at: 177 177
Size: 3407
EcoO109I rG'GnC_Cy
Cuts at: 546 546
Size: 3407
EcoRI G'AATT_C
Cuts at: 411 411
Size: 3407
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1426 1426
Size: 3407
FspI TGC'GCA
Cuts at: 1188 1188
Size: 3407
HaeII r_GCGC'y
Cuts at: 841 1089 2111 3090 841
Size: 248 1022 979 1158
Fragments arranged by size:
1158 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 253 285 1249 1279 1281 1311 253
Size: 32 964 30 2 30 2349
Fragments arranged by size:
2349 964 32 30 30 2
HincII GTy'rAC
Cuts at: 213 446 213
Size: 233 3174
KasI G'GCGC_C
Cuts at: 837 1085 837
Size: 248 3159
KpnI G_GTAC'C
Cuts at: 427 427
Size: 3407
MluI A'CGCG_T
Cuts at: 2840 2840
Size: 3407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2078 2262 2078
Size: 184 3223
MscI TGG'CCA
Cuts at: 358 1168 358
Size: 810 2597
MslI CAynn'nnrTG
Cuts at: 1396 1523 1396
Size: 127 3280
MspA1I CmG'CkG
Cuts at: 518 832 1192 2205 2450 518
Size: 314 360 1013 245 1475
Fragments arranged by size:
1475 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1588 1588
Size: 3407
NarI GG'CG_CC
Cuts at: 838 1086 838
Size: 248 3159
NcoI C'CATG_G
Cuts at: 107 107
Size: 3407
NgoMIV G'CCGG_C
Cuts at: 1586 1586
Size: 3407
NspI r_CATG'y
Cuts at: 460 960 1867 460
Size: 500 907 2000
Fragments arranged by size:
2000 907 500
PciI A'CATG_T
Cuts at: 1863 1863
Size: 3407
PmeI GTTT'AAAC
Cuts at: 406 406
Size: 3407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 307 339 2546 2578 307
Size: 32 2207 32 1136
Fragments arranged by size:
2207 1136 32 32
PshAI GACnn'nnGTC
Cuts at: 154 154
Size: 3407
PstI C_TGCA'G
Cuts at: 454 1139 454
Size: 685 2722
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3407
PvuII CAG'CTG
Cuts at: 832 1192 832
Size: 360 3047
RsrII CG'GwC_CG
Cuts at: 1602 1602
Size: 3407
SacI G_AGCT'C
Cuts at: 320 421 320
Size: 101 3306
SalI G'TCGA_C
Cuts at: 444 444
Size: 3407
SapI GCTCTTCn'nnn_
Cuts at: 283 1430 1640 283
Size: 1147 210 2050
Fragments arranged by size:
2050 1147 210
SbfI CC_TGCA'GG
Cuts at: 454 454
Size: 3407
ScaI AGT'ACT
Cuts at: 334 2924 334
Size: 2590 817
SfcI C'TryA_G
Cuts at: 33 450 1135 2128 2319 33
Size: 417 685 993 191 1121
Fragments arranged by size:
1121 993 685 417 191
SfoI GGC'GCC
Cuts at: 839 1087 839
Size: 248 3159
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3407
SmaI CCC'GGG
Cuts at: 429 429
Size: 3407
SmlI C'TyrA_G
Cuts at: 256 319 562 1477 1969 2231 256
Size: 63 243 915 492 262 1432
Fragments arranged by size:
1432 915 492 262 243 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3407
SphI G_CATG'C
Cuts at: 460 960 460
Size: 500 2907
StyI C'CwwG_G
Cuts at: 107 359 541 895 107
Size: 252 182 354 2619
Fragments arranged by size:
2619 354 252 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 990 1123 1582 1791 990
Size: 133 459 209 2606
Fragments arranged by size:
2606 459 209 133
TatI w'GTAC_w
Cuts at: 332 2922 332
Size: 2590 817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3375
Tth111I GACn'n_nGTC
Cuts at: 1204 1204
Size: 3407
XbaI T'CTAG_A
Cuts at: 438 438
Size: 3407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 902 150
Size: 752 2655
XmaI C'CCGG_G
Cuts at: 427 427
Size: 3407
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PshAI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmgBI
BmtI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI