Restriction map for pF1KB8848
 (Circular) (Six-base) MAPSORT of: pF1KB8848.seq  Check: 5498  from: 1  to: 3314



pF1KB8848  3314 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:30  ..

AccI GT'mk_AC

Cuts at:    352     352 
   Size:       3314
 
Acc65I G'GTAC_C

Cuts at:    330     330 
   Size:       3314
 
AclI AA'CG_TT

Cuts at:   3154    3154 
   Size:       3314
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    247    1139    1571    2318    2586     247 
   Size:        892     432     747     268     975

  Fragments arranged by size: 

                 975     892     747     432     268
 
AfeI AGC'GCT

Cuts at:    232     232 
   Size:       3314
 
AflIII A'CryG_T

Cuts at:    666    1770    2747     666 
   Size:       1104     977    1233

  Fragments arranged by size: 

                1233    1104     977
 
AgeI A'CCGG_T

Cuts at:   1702    1702 
   Size:       3314
 
AlwNI CAG_nnn'CTG

Cuts at:    619    2186     619 
   Size:       1567    1747
 
ApaLI G'TGCA_C

Cuts at:   2084    2084 
   Size:       3314
 
ApoI r'AATT_y

Cuts at:    318    2667    2923     318 
   Size:       2349     256     709

  Fragments arranged by size: 

                2349     709     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3314
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3314
 
AvaI C'yCGr_G

Cuts at:    334     334 
   Size:       3314
 
BamHI G'GATC_C

Cuts at:    339     339 
   Size:       3314
 
BanI G'GyrC_C

Cuts at:    330     744     992    1027     330 
   Size:        414     248      35    2617

  Fragments arranged by size: 

                2617     414     248      35
 
BanII G_rGCy'C

Cuts at:    328    1358     328 
   Size:       1030    2284
 
BbeI G_GCGC'C

Cuts at:    748     996     748 
   Size:        248    3066
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    129     163     187     221     129 
   Size:         34      24      34    3222

  Fragments arranged by size: 

                3222      34      34      24
 
BciVI GTATCCnnnnn_n'

Cuts at:   1212    1973    3314    1212 
   Size:        761    1341    1212

  Fragments arranged by size: 

                1341    1212     761
 
BclI T'GATC_A

Cuts at:    110     834     110 
   Size:        724    2590
 
BglII A'GATC_T

Cuts at:    829    2866     829 
   Size:       2037    1277
 
BlpI GC'TnA_GC

Cuts at:    426     426 
   Size:       3314
 
Bme1580I G_kGCm'C

Cuts at:    939    1032    2088     939 
   Size:         93    1056    2165

  Fragments arranged by size: 

                2165    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    526     607     942    3086     526 
   Size:         81     335    2144     754

  Fragments arranged by size: 

                2144     754     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1681    1681 
   Size:       3314
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    322     322 
   Size:       3314
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    490    1369    1861    2159     490 
   Size:        879     492     298    1645

  Fragments arranged by size: 

                1645     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3018    3018 
   Size:       3314
 
BsaAI yAC'GTr

Cuts at:    264     667     264 
   Size:        403    2911
 
BsaBI GATnn'nnATC

Cuts at:      6     828    2865       6 
   Size:        822    2037     455

  Fragments arranged by size: 

                2037     822     455
 
BsaHI Gr'CG_yC

Cuts at:    745     993    1695    3191     745 
   Size:        248     702    1496     868

  Fragments arranged by size: 

                1496     868     702     248
 
BsaWI w'CCGG_w

Cuts at:    503    1024    1702    1976    2123    2534     503 
   Size:        521     678     274     147     411    1283

  Fragments arranged by size: 

                1283     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    739    1497    2074    2570     739 
   Size:        758     577     496    1483

  Fragments arranged by size: 

                1483     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     856     902    2110     104 
   Size:        752      46    1208    1308

  Fragments arranged by size: 

                1308    1208     752      46
 
BsiHKAI G_wGCw'C

Cuts at:    328    1106    1296    2088     328 
   Size:        778     190     792    1554

  Fragments arranged by size: 

                1554     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    328     939    1032    1106    1296    1358    2088     328 
   Size:        611      93      74     190      62     730    1554

  Fragments arranged by size: 

                1554     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2534    2534 
   Size:       3314
 
BspHI T'CATG_A

Cuts at:   2490    2490 
   Size:       3314
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    364     578     880    1261     364 
   Size:        214     302     381    2417

  Fragments arranged by size: 

                2417     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1606    1660    3144    3309      50 
   Size:       1556      54    1484     165      55

  Fragments arranged by size: 

                1556    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1226    1226 
   Size:       3314
 
BsrFI r'CCGG_y

Cuts at:   1312    1493    1702    1312 
   Size:        181     209    2924

  Fragments arranged by size: 

                2924     209     181
 
BssSI C'ACGA_G

Cuts at:   1585    1943    1585 
   Size:        358    2956
 
BstAPI GCAn_nnn'nTGC

Cuts at:    691     691 
   Size:       3314
 
BstBI TT'CG_AA

Cuts at:   1675    1675 
   Size:       3314
 
BstYI r'GATC_y

Cuts at:      1     339     806     829    1164    1410    2422    2508    2520 
   Size:        338     467      23     335     246    1012      86      12

Cuts at:   2520    2866       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     449     346     338     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1264    1445    1608    1264 
   Size:        181     163    2970

  Fragments arranged by size: 

                2970     181     163
 
BtsI GCAGTG_nn'

Cuts at:    557     557 
   Size:       3314
 
DraI TTT'AAA

Cuts at:    313     313 
   Size:       3314
 
DrdI GACnn_nn'nnGTC

Cuts at:   1020    1878    1020 
   Size:        858    2456
 
EaeI y'GGCC_r

Cuts at:    899    1073    1464    1491    2836     899 
   Size:        174     391      27    1345    1377

  Fragments arranged by size: 

                1377    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    899     899 
   Size:       3314
 
EarI CTCTTCn'nnn_

Cuts at:   1337    1547    1337 
   Size:        210    3104
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1522    1832    1978    3124    1522 
   Size:        310     146    1146    1712

  Fragments arranged by size: 

                1712    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    326     326 
   Size:       3314
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    247     322    1139    1571    2318    2586     247 
   Size:         75     817     432     747     268     975

  Fragments arranged by size: 

                 975     817     747     432     268      75
 
EcoNI CCTnn'n_nnAGG

Cuts at:    304     304 
   Size:       3314
 
EcoO109I rG'GnC_Cy

Cuts at:    453     453 
   Size:       3314
 
EcoRI G'AATT_C

Cuts at:    318     318 
   Size:       3314
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1333    1333 
   Size:       3314
 
FspI TGC'GCA

Cuts at:   1095    1095 
   Size:       3314
 
HaeII r_GCGC'y

Cuts at:    234     748     996    2018    2997     234 
   Size:        514     248    1022     979     551

  Fragments arranged by size: 

                1022     979     551     514     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1156    1186    1188    1218    1156 
   Size:         30       2      30    3252

  Fragments arranged by size: 

                3252      30      30       2
 
HincII GTy'rAC

Cuts at:    353     353 
   Size:       3314
 
KasI G'GCGC_C

Cuts at:    744     992     744 
   Size:        248    3066
 
KpnI G_GTAC'C

Cuts at:    334     334 
   Size:       3314
 
MluI A'CGCG_T

Cuts at:   2747    2747 
   Size:       3314
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1985    2169    1985 
   Size:        184    3130
 
MscI TGG'CCA

Cuts at:   1075    1075 
   Size:       3314
 
MslI CAynn'nnrTG

Cuts at:   1303    1430    1303 
   Size:        127    3187
 
MspA1I CmG'CkG

Cuts at:    425     739    1099    2112    2357     425 
   Size:        314     360    1013     245    1382

  Fragments arranged by size: 

                1382    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1495    1495 
   Size:       3314
 
NarI GG'CG_CC

Cuts at:    745     993     745 
   Size:        248    3066
 
NgoMIV G'CCGG_C

Cuts at:   1493    1493 
   Size:       3314
 
NspI r_CATG'y

Cuts at:    367     867    1774     367 
   Size:        500     907    1907

  Fragments arranged by size: 

                1907     907     500
 
PasI CC'CwG_GG

Cuts at:    219     219 
   Size:       3314
 
PciI A'CATG_T

Cuts at:   1770    1770 
   Size:       3314
 
PmeI GTTT'AAAC

Cuts at:    313     313 
   Size:       3314
 
PmlI CAC'GTG

Cuts at:    264     264 
   Size:       3314
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2453    2485    2453 
   Size:         32    3282
 
PstI C_TGCA'G

Cuts at:    185     361    1046     185 
   Size:        176     685    2453

  Fragments arranged by size: 

                2453     685     176
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3314
 
PvuII CAG'CTG

Cuts at:    739    1099     739 
   Size:        360    2954
 
RsrII CG'GwC_CG

Cuts at:   1509    1509 
   Size:       3314
 
SacI G_AGCT'C

Cuts at:    328     328 
   Size:       3314
 
SalI G'TCGA_C

Cuts at:    351     351 
   Size:       3314
 
SapI GCTCTTCn'nnn_

Cuts at:   1337    1547    1337 
   Size:        210    3104
 
SbfI CC_TGCA'GG

Cuts at:    361     361 
   Size:       3314
 
ScaI AGT'ACT

Cuts at:   2831    2831 
   Size:       3314
 
SfcI C'TryA_G

Cuts at:     33     181     357    1042    2035    2226      33 
   Size:        148     176     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     191     176     148
 
SfoI GGC'GCC

Cuts at:    746     994     746 
   Size:        248    3066
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3314
 
SmaI CCC'GGG

Cuts at:    336     336 
   Size:       3314
 
SmlI C'TyrA_G

Cuts at:    469    1384    1876    2138     469 
   Size:        915     492     262    1645

  Fragments arranged by size: 

                1645     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3314
 
SphI G_CATG'C

Cuts at:    367     867     367 
   Size:        500    2814
 
StyI C'CwwG_G

Cuts at:    448     802     448 
   Size:        354    2960
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    897    1030    1489    1698     897 
   Size:        133     459     209    2513

  Fragments arranged by size: 

                2513     459     209     133
 
TatI w'GTAC_w

Cuts at:    191    2829     191 
   Size:       2638     676
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2831    2863    2831 
   Size:         32    3282
 
Tth111I GACn'n_nGTC

Cuts at:   1111    1111 
   Size:       3314
 
XbaI T'CTAG_A

Cuts at:    345     345 
   Size:       3314
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    809     809 
   Size:       3314
 
XmaI C'CCGG_G

Cuts at:    334     334 
   Size:       3314
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NspI     PasI     PciI     PmeI     PmlI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI
     BmtI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI
    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI    PflMI
     PfoI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com