Restriction map for pF1KB8842
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BalI        
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BlnI        
BpuAI       BsaAI       BsaBI       BsaHI       BseAI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      BssSI       BstBI       BstUI       Bsu36I      
CfoI        ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     KasI        
Ksp632I     KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NarI        NciI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tsp509I     Tth111I     Van91I      XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        808  GGTACC               G^GTACC
Asp718I       808  GGTACC               G^GTACC
BamHI         441  GGATCC               G^GATCC
BanI          808  GGTACC               G^G[CT][GA]CC
BanII         109  GGGCTC               G[GA]GC[CT]^C
BglII         230  AGATCT               A^GATCT
BlpI          608  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      608  GCTCAGC              GC^T[ACGT]AGC
BsaI         1303  GAGACC               GGTCTC(1/5)
BsaWI         245  ACCGGA               [AT]^CCGG[AT]
BsiEI         708  CGACCG               CG[GA][CT]^CG
BsmFI         552  GTCCC                GGGAC(10/14)
BsrBI        1387  GAGCGG               CCGCTC(-3/-3)
BsrFI        1239  GCCGGC               [GA]^CCGG[CT]
Bst1107I      639  GTATAC               GTA^TAC
BstEII        777  GGTCACC              G^GT[ACGT]ACC
BstPI         777  GGTCACC              G^GT[ACGT]ACC
BstZ17I       639  GTATAC               GTA^TAC
CelII         608  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1239  GCCGGC               [GA]^CCGG[CT]
DraIII        600  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1415  TGGCCG               [CT]^GGCC[GA]
EcoO65I       777  GGTCACC              G^GT[ACGT]ACC
HincII        154  GTTAAC               GT[CT]^[GA]AC
HindII        154  GTTAAC               GT[CT]^[GA]AC
HpaI          154  GTTAAC               GTT^AAC
KpnI          808  GGTACC               GGTAC^C
MboII        1105  GAAGA                GAAGA(8/7)
MslI          432  CATCCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1239  GCCGGC               GCC^GGC
NcoI           86  CCATGG               C^CATGG
NgoMIV       1239  GCCGGC               G^CCGGC
PaeR7I        772  CTCGAG               C^TCGAG
PpuMI         494  AGGTCCT              [GA]G^G[AT]CC[CT]
SexAI         149  ACCAGGT              A^CC[AT]GGT
StuI          369  AGGCCT               AGG^CCT
TaqI          773  TCGA                 T^CGA
TfiI          967  GAATC                G^A[AT]TC
TthHB8I       773  TCGA                 T^CGA
XbaI         1272  TCTAGA               T^CTAGA
XcmI           77  CCATCTCTCCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          772  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccI          334  GTCTAC               GT^[AC][GT]AC
              639  GTATAC              
AfaI          428  GTAC                 GT^AC
              809  GTAC                
AlwNI         892  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1096  CAGATCCTG           
BbvCI         792  CCTCAGC              CCTCAGC(-5/-2)
              490  GCTGAGG             
BseRI        1342  GAGGAG               GAGGAG(10/8)
             1378  GAGGAG              
BsmAI           9  GAGAC                GTCTC(1/5)
             1303  GAGAC               
BspMI        1172  ACCTGC               ACCTGC(4/8)
             1443  GCAGGT              
BstAPI        599  GCACAATGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1255  GCAGGCTGTGC         
BstXI         431  CCATCCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              798  CCAGTTCTTTGG        
EcoNI         114  CCTGATTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1335  CCTCACCGAGG         
FauI         1208  CCCGC                CCCGC(4/6)
             1407  GCGGG               
HapII         246  CCGG                 C^CGG
             1240  CCGG                
HpaII         246  CCGG                 C^CGG
             1240  CCGG                
MspI          246  CCGG                 C^CGG
             1240  CCGG                
NspI          854  ACATGC               [GA]CATG^[CT]
             1232  ACATGC              
RsaI          428  GTAC                 GT^AC
              809  GTAC                
Tsp45I        778  GTCAC                ^GT[GC]AC
             1327  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       AvaI        
AvaII       BbvI        BfaI        BmyI        BpmI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       DsaI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MaeI        MaeIII      MboI        
MflI        MnlI        MseI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PstI        PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        Tru9I       
TseI        TspRI       XhoII       
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