Restriction map for
pF1KB8839
(Circular) (Six-base) MAPSORT of: pF1KB8839.seq Check: 1322 from: 1 to: 4511
pF1KB8839 4511 bp
With 182 enzymes: SgfI *
June 20, 2008 10:30 ..
AccI GT'mk_AC
Cuts at: 1549 1549
Size: 4511
Acc65I G'GTAC_C
Cuts at: 1527 1527
Size: 4511
AclI AA'CG_TT
Cuts at: 4351 4351
Size: 4511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1139 2336 2768 3515 3783 1139
Size: 1197 432 747 268 1867
Fragments arranged by size:
1867 1197 747 432 268
AflIII A'CryG_T
Cuts at: 1863 2967 3944 1863
Size: 1104 977 2430
Fragments arranged by size:
2430 1104 977
AgeI A'CCGG_T
Cuts at: 2899 2899
Size: 4511
AlwNI CAG_nnn'CTG
Cuts at: 1816 3383 1816
Size: 1567 2944
ApaI G_GGCC'C
Cuts at: 1311 1311
Size: 4511
ApaLI G'TGCA_C
Cuts at: 1346 3281 1346
Size: 1935 2576
ApoI r'AATT_y
Cuts at: 1515 3864 4120 1515
Size: 2349 256 1906
Fragments arranged by size:
2349 1906 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4511
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
AvaI C'yCGr_G
Cuts at: 737 1247 1531 737
Size: 510 284 3717
Fragments arranged by size:
3717 510 284
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 462 495 1101 1134 462
Size: 33 606 33 3839
Fragments arranged by size:
3839 606 33 33
BamHI G'GATC_C
Cuts at: 199 909 1536 199
Size: 710 627 3174
Fragments arranged by size:
3174 710 627
BanI G'GyrC_C
Cuts at: 1090 1527 1941 2189 2224 1090
Size: 437 414 248 35 3377
Fragments arranged by size:
3377 437 414 248 35
BanII G_rGCy'C
Cuts at: 576 955 1311 1525 2555 576
Size: 379 356 214 1030 2532
Fragments arranged by size:
2532 1030 379 356 214
BbeI G_GCGC'C
Cuts at: 1945 2193 1945
Size: 248 4263
BbsI GAAGACnn'nnnn_
Cuts at: 1145 1459 1145
Size: 314 4197
BciVI GTATCCnnnnn_n'
Cuts at: 2409 3170 4511 2409
Size: 761 1341 2409
Fragments arranged by size:
2409 1341 761
BclI T'GATC_A
Cuts at: 704 2031 704
Size: 1327 3184
BfrBI ATG'CAT
Cuts at: 1079 1079
Size: 4511
BglII A'GATC_T
Cuts at: 2026 4063 2026
Size: 2037 2474
BlpI GC'TnA_GC
Cuts at: 1623 1623
Size: 4511
Bme1580I G_kGCm'C
Cuts at: 1311 1350 2136 2229 3285 1311
Size: 39 786 93 1056 2537
Fragments arranged by size:
2537 1056 786 93 39
BmrI ACTGGGnnnn_n'
Cuts at: 496 1723 1804 2139 4283 496
Size: 1227 81 335 2144 724
Fragments arranged by size:
2144 1227 724 335 81
BmtI G_CTAG'C
Cuts at: 409 409
Size: 4511
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2878 2878
Size: 4511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 560 892 253
Size: 307 332 3872
Fragments arranged by size:
3872 332 307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1687 2566 3058 3356 1687
Size: 879 492 298 2842
Fragments arranged by size:
2842 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4215 4215
Size: 4511
BsaAI yAC'GTr
Cuts at: 1864 1864
Size: 4511
BsaBI GATnn'nnATC
Cuts at: 6 2025 4062 6
Size: 2019 2037 455
Fragments arranged by size:
2037 2019 455
BsaHI Gr'CG_yC
Cuts at: 1942 2190 2892 4388 1942
Size: 248 702 1496 2065
Fragments arranged by size:
2065 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1700 2221 2899 3173 3320 3731 1700
Size: 521 678 274 147 411 2480
Fragments arranged by size:
2480 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 117 955 1229 117
Size: 838 274 3399
Fragments arranged by size:
3399 838 274
BseYI C'CCAG_C
Cuts at: 853 1936 2694 3271 3767 853
Size: 1083 758 577 496 1597
Fragments arranged by size:
1597 1083 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2053 2099 3307 104
Size: 1949 46 1208 1308
Fragments arranged by size:
1949 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 878 955 1331 1350 1525 2303 2493 3285 878
Size: 77 376 19 175 778 190 792 2104
Fragments arranged by size:
2104 792 778 376 190 175 77 19
Bsp1286I G_dGCh'C
Cuts at: 576 878 955 1311 1331 1350 1525 2136 2229
Size: 302 77 356 20 19 175 611 93
Cuts at: 2229 2303 2493 2555 3285 576
Size: 74 190 62 730 1802
Fragments arranged by size:
1802 730 611 356 302 190 175 93
77 74 62 20 19
BspEI T'CCGG_A
Cuts at: 3731 3731
Size: 4511
BspHI T'CATG_A
Cuts at: 710 3687 710
Size: 2977 1534
BspMI ACCTGCnnnn'nnnn_
Cuts at: 517 1561 1775 2077 2458 517
Size: 1044 214 302 381 2570
Fragments arranged by size:
2570 1044 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2803 2857 4341 4506 50
Size: 2753 54 1484 165 55
Fragments arranged by size:
2753 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 435 1443 2423 435
Size: 1008 980 2523
Fragments arranged by size:
2523 1008 980
BsrFI r'CCGG_y
Cuts at: 2509 2690 2899 2509
Size: 181 209 4121
Fragments arranged by size:
4121 209 181
BsrGI T'GTAC_A
Cuts at: 344 344
Size: 4511
BssSI C'ACGA_G
Cuts at: 2782 3140 2782
Size: 358 4153
BstAPI GCAn_nnn'nTGC
Cuts at: 1888 1888
Size: 4511
BstBI TT'CG_AA
Cuts at: 2872 2872
Size: 4511
BstEII G'GTnAC_C
Cuts at: 213 213
Size: 4511
BstXI CCAn_nnnn'nTGG
Cuts at: 522 1041 522
Size: 519 3992
BstYI r'GATC_y
Cuts at: 1 199 909 1536 2003 2026 2361 2607 3619
Size: 198 710 627 467 23 335 246 1012
Cuts at: 3619 3705 3717 4063 1
Size: 86 12 346 449
Fragments arranged by size:
1012 710 627 467 449 346 335 246
198 86 23 12
BtgI C'CryG_G
Cuts at: 1136 1136
Size: 4511
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2461 2642 2805 2461
Size: 181 163 4167
Fragments arranged by size:
4167 181 163
BtsI GCAGTG_nn'
Cuts at: 864 1754 864
Size: 890 3621
DraI TTT'AAA
Cuts at: 1461 1510 1461
Size: 49 4462
DraIII CAC_nnn'GTG
Cuts at: 1370 1370
Size: 4511
DrdI GACnn_nn'nnGTC
Cuts at: 421 2217 3075 421
Size: 1796 858 1857
Fragments arranged by size:
1857 1796 858
EaeI y'GGCC_r
Cuts at: 2096 2270 2661 2688 4033 2096
Size: 174 391 27 1345 2574
Fragments arranged by size:
2574 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2096 2096
Size: 4511
EarI CTCTTCn'nnn_
Cuts at: 592 2534 2744 592
Size: 1942 210 2359
Fragments arranged by size:
2359 1942 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2719 3029 3175 4321 2719
Size: 310 146 1146 2909
Fragments arranged by size:
2909 1146 310 146
EcoICRI GAG'CTC
Cuts at: 953 1523 953
Size: 570 3941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 560 892 1139 2336 2768 3515 3783 253
Size: 307 332 247 1197 432 747 268 981
Fragments arranged by size:
1197 981 747 432 332 307 268 247
EcoO109I rG'GnC_Cy
Cuts at: 382 552 1308 1650 382
Size: 170 756 342 3243
Fragments arranged by size:
3243 756 342 170
EcoRI G'AATT_C
Cuts at: 1515 1515
Size: 4511
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1473 2530 1473
Size: 1057 3454
FspI TGC'GCA
Cuts at: 2292 2292
Size: 4511
HaeII r_GCGC'y
Cuts at: 1499 1945 2193 3215 4194 1499
Size: 446 248 1022 979 1816
Fragments arranged by size:
1816 1022 979 446 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 952 984 1048 1080 2353 2383 2385 2415 952
Size: 32 64 32 1273 30 2 30 3048
Fragments arranged by size:
3048 1273 64 32 32 30 30 2
HincII GTy'rAC
Cuts at: 258 279 1550 258
Size: 21 1271 3219
Fragments arranged by size:
3219 1271 21
KasI G'GCGC_C
Cuts at: 1941 2189 1941
Size: 248 4263
KpnI G_GTAC'C
Cuts at: 1531 1531
Size: 4511
MfeI C'AATT_G
Cuts at: 1376 1376
Size: 4511
MluI A'CGCG_T
Cuts at: 3944 3944
Size: 4511
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3182 3366 3182
Size: 184 4327
MscI TGG'CCA
Cuts at: 2272 2272
Size: 4511
MslI CAynn'nnrTG
Cuts at: 520 715 2500 2627 520
Size: 195 1785 127 2404
Fragments arranged by size:
2404 1785 195 127
MspA1I CmG'CkG
Cuts at: 544 1622 1936 2296 3309 3554 544
Size: 1078 314 360 1013 245 1501
Fragments arranged by size:
1501 1078 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2692 2692
Size: 4511
NarI GG'CG_CC
Cuts at: 1942 2190 1942
Size: 248 4263
NgoMIV G'CCGG_C
Cuts at: 2690 2690
Size: 4511
NheI G'CTAG_C
Cuts at: 405 405
Size: 4511
NsiI A_TGCA'T
Cuts at: 1081 1081
Size: 4511
NspI r_CATG'y
Cuts at: 852 1079 1564 2064 2971 852
Size: 227 485 500 907 2392
Fragments arranged by size:
2392 907 500 485 227
PasI CC'CwG_GG
Cuts at: 905 1041 905
Size: 136 4375
PciI A'CATG_T
Cuts at: 2967 2967
Size: 4511
PmeI GTTT'AAAC
Cuts at: 1510 1510
Size: 4511
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3650 3682 3650
Size: 32 4479
PpuMI rG'GwC_Cy
Cuts at: 382 552 382
Size: 170 4341
PsiI TTA'TAA
Cuts at: 161 1484 161
Size: 1323 3188
PspOMI G'GGCC_C
Cuts at: 1307 1307
Size: 4511
PstI C_TGCA'G
Cuts at: 1558 2243 1558
Size: 685 3826
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4511
PvuII CAG'CTG
Cuts at: 544 1936 2296 544
Size: 1392 360 2759
Fragments arranged by size:
2759 1392 360
RsrII CG'GwC_CG
Cuts at: 2706 2706
Size: 4511
SacI G_AGCT'C
Cuts at: 955 1525 955
Size: 570 3941
SalI G'TCGA_C
Cuts at: 1548 1548
Size: 4511
SapI GCTCTTCn'nnn_
Cuts at: 2534 2744 2534
Size: 210 4301
SbfI CC_TGCA'GG
Cuts at: 1558 1558
Size: 4511
ScaI AGT'ACT
Cuts at: 4028 4028
Size: 4511
SfcI C'TryA_G
Cuts at: 33 1554 2239 3232 3423 33
Size: 1521 685 993 191 1121
Fragments arranged by size:
1521 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1943 2191 1943
Size: 248 4263
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4511
SmaI CCC'GGG
Cuts at: 1249 1533 1249
Size: 284 4227
SmlI C'TyrA_G
Cuts at: 1666 2581 3073 3335 1666
Size: 915 492 262 2842
Fragments arranged by size:
2842 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4511
SphI G_CATG'C
Cuts at: 1564 2064 1564
Size: 500 4011
SspI AAT'ATT
Cuts at: 1185 1185
Size: 4511
StyI C'CwwG_G
Cuts at: 1645 1999 1645
Size: 354 4157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2094 2227 2686 2895 2094
Size: 133 459 209 3710
Fragments arranged by size:
3710 459 209 133
TatI w'GTAC_w
Cuts at: 344 4026 344
Size: 3682 829
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 374 406 616 648 812 844 1340 1372 4028
Size: 32 210 32 164 32 496 32 2656
Cuts at: 4028 4060 374
Size: 32 825
Fragments arranged by size:
2656 825 496 210 164 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2308 2308
Size: 4511
XbaI T'CTAG_A
Cuts at: 1542 1542
Size: 4511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1272 2006 1272
Size: 734 3777
XmaI C'CCGG_G
Cuts at: 1247 1531 1247
Size: 284 4227
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NsiI NspI PasI PciI
PmeI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BbvCI BcgI BglI BmgBI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI NcoI
NdeI NotI NruI PacI PflMI PfoI PmlI PshAI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI