Restriction map for
pF1KB8838
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# NO CUT ENZYMES ( 107 enzymes) #
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Acc65I AccI AccIII AclI AflII
AflIII Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BseAI BseRI BsgI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MaeI MamI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SexAI SfuI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tru9I Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1126 GACGTC GACGT^C
AcsI 1141 AAATTC [GA]^AATT[CT]
AgeI 1099 ACCGGT A^CCGGT
AhdI 370 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1141 AAATTC [GA]^AATT[CT]
Asp700I 1273 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 370 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 63 GAGCTC G[AT]GC[AT]^C
BamHI 546 GGATCC G^GATCC
BbeI 55 GGCGCC GGCGC^C
BglI 421 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1065 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1065 GCTGAGC GC^T[ACGT]AGC
BsaI 712 GGTCTC GGTCTC(1/5)
BsaWI 1099 ACCGGT [AT]^CCGG[AT]
BsiHKAI 63 GAGCTC G[AT]GC[AT]^C
BsmBI 984 CGTCTC CGTCTC(1/5)
BsmI 395 GCATTC GAATGC(1/-1)
BssHII 391 GCGCGC G^CGCGC
CelII 1065 GCTGAGC GC^T[ACGT]AGC
DraIII 342 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 130 CGGCCG C^GGCCG
Eam1105I 370 GACGTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 130 CGGCCG C^GGCCG
Eco52I 130 CGGCCG C^GGCCG
HincII 289 GTCAAC GT[CT]^[GA]AC
HindII 289 GTCAAC GT[CT]^[GA]AC
KasI 55 GGCGCC G^GCGCC
KspI 41 CCGCGG CCGC^GG
NaeI 132 GCCGGC GCC^GGC
NarI 55 GGCGCC GG^CGCC
NgoMIV 132 GCCGGC G^CCGGC
PaeR7I 1037 CTCGAG C^TCGAG
PflMI 822 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1099 ACCGGT A^CCGGT
PpuMI 1185 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1352 CTGCAG CTGCA^G
PvuII 532 CAGCTG CAG^CTG
SacI 63 GAGCTC GAGCT^C
SacII 41 CCGCGG CCGC^GG
SapI 1116 GCTCTTC GCTCTTC(1/4)
SbfI 1351 CCTGCAGG CCTGCA^GG
SfaNI 452 GCATC GCATC(5/9)
SfcI 1352 CTGCAG C^T[GA][CT]AG
SfiI 420 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 55 GGCGCC GGC^GCC
SgrAI 495 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 1351 CCTGCAGG CCTGCA^GG
TfiI 471 GAATC G^A[AT]TC
Van91I 822 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1037 CTCGAG C^TCGAG
XmnI 1273 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 55 GGCGCC G[GA]^CG[CT]C
1126 GACGTC
AfaI 238 GTAC GT^AC
960 GTAC
AvaI 250 CCCGAG C^[CT]CG[GA]G
1037 CTCGAG
BsaHI 55 GGCGCC G[GA]^CG[CT]C
1126 GACGTC
BsmAI 713 GTCTC GTCTC(1/5)
985 GTCTC
BsmFI 410 GGGAC GGGAC(10/14)
1185 GGGAC
BsoBI 250 CCCGAG C^[CT]CG[GA]G
1037 CTCGAG
BspMI 1188 ACCTGC ACCTGC(4/8)
327 GCAGGT
BsrBI 627 GAGCGG CCGCTC(-3/-3)
833 GAGCGG
BstXI 323 CCAGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
512 CCAACAGCCTGG
EarI 1117 CTCTTC CTCTTC(1/4)
1179 CTCTTC
HgaI 483 GACGC GACGC(5/10)
304 GCGTC
Hin1I 55 GGCGCC G[GA]^CG[CT]C
1126 GACGTC
HphI 97 GGTGA GGTGA(8/7)
1251 GGTGA
Ksp632I 1117 CTCTTC CTCTTC(1/4)
1179 CTCTTC
NcoI 570 CCATGG C^CATGG
1362 CCATGG
PleI 376 GAGTC GAGTC(4/5)
1174 GAGTC
RsaI 238 GTAC GT^AC
960 GTAC
SmlI 115 CTCAAG C^T[CT][GA]AG
1037 CTCGAG
StuI 1356 AGGCCT AGG^CCT
1377 AGGCCT
Tsp45I 306 GTCAC ^GT[GC]AC
611 GTGAC
Tsp509I 1142 AATT ^AATT
1255 AATT
XcmI 204 CCAGGGCTTCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
479 CCAAGACGCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AvaII BalI BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiEI BsiYI BslI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
StyI TaqI TseI TspRI TthHB8I
XhoII