Restriction map for pF1KB8830
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       Cfr10I      CpoI        DraI        DraIII      
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HapII       Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KspI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SphI        Sse8387I    StuI        StyI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I        916  GGTACC               G^GTACC
Asp700I      1120  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       916  GGTACC               G^GTACC
BanI          916  GGTACC               G^G[CT][GA]CC
BanII        1075  GGGCTC               G[GA]GC[CT]^C
BmyI         1075  GGGCTC               G[AGT]GC[ACT]^C
BsaJI         293  CCGTGG               C^C[ACGT][ACGT]GG
Bsp1286I     1075  GGGCTC               G[AGT]GC[ACT]^C
Bsp1407I     1198  TGTACA               T^GTACA
BsrDI        1314  GCAATG               GCAATG(2/0)
BsrGI        1198  TGTACA               T^GTACA
BssKI         648  CCTGG                ^CC[ACGT]GG
BstNI         648  CCTGG                CC^[AT]GG
CfoI         1103  GCGC                 GCG^C
DsaI          293  CCGTGG               C^C[GA][CT]GG
EcoRII        648  CCTGG                ^CC[AT]GG
HaeII        1102  AGCGCC               [GA]GCGC^[CT]
HgaI          974  GACGC                GACGC(5/10)
HhaI         1103  GCGC                 GCG^C
HinP1I       1103  GCGC                 G^CGC
KpnI          916  GGTACC               GGTAC^C
MspA1I        207  CAGCTG               C[AC]G^C[GT]G
MvaI          648  CCTGG                CC^[AT]GG
MwoI          883  GCAGCCGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PleI          934  GACTC                GAGTC(4/5)
PvuII         207  CAGCTG               CAG^CTG
ScaI         1287  AGTACT               AGT^ACT
ScrFI         648  CCTGG                CC^[ACGT]GG
SexAI         647  ACCTGGT              A^CC[AT]GGT
SpeI           52  ACTAGT               A^CTAGT
SspBI        1198  TGTACA               T^GTACA
SspI           19  AATATT               AAT^ATT
XcmI          740  CCAAATGGGATTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1120  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwNI         140  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              277  CAGAAACTG           
AvaII         622  GGTCC                G^G[AT]CC
             1095  GGACC               
BbsI          946  GAAGAC               GAAGAC(2/6)
             1051  GTCTTC              
BbvI          883  GCAGC                GCAGC(8/12)
             1167  GCAGC               
BpuAI         946  GAAGAC               GAAGAC(2/6)
             1051  GTCTTC              
BspDI         251  ATCGAT               AT^CGAT
             1216  ATCGAT              
BstXI         218  CCATTGAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              853  CCATGTTACTGG        
ClaI          251  ATCGAT               AT^CGAT
             1216  ATCGAT              
DraII         621  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1094  AGGACCC             
DrdI          974  GACGCTATAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1042  GACCCCTGTGTC        
EarI          336  CTCTTC               CTCTTC(1/4)
              989  CTCTTC              
EcoO109I      621  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1094  AGGACCC             
Fnu4HI        883  GCAGC                GC^[ACGT]GC
             1167  GCAGC               
HaeIII         44  GGCC                 GG^CC
              262  GGCC                
ItaI          883  GCAGC                GC^[ACGT]GC
             1167  GCAGC               
Ksp632I       336  CTCTTC               CTCTTC(1/4)
              989  CTCTTC              
MaeIII        857  GTTAC                ^GT[ACGT]AC
             1193  GTAAC               
PpuMI         621  AGGTCCT              [GA]G^G[AT]CC[CT]
             1094  AGGACCC             
PstI          766  CTGCAG               CTGCA^G
             1165  CTGCAG              
TfiI          685  GATTC                G^A[AT]TC
             1014  GAATC               
TseI          883  GCAGC                G^C[AT]GC
             1167  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        BfaI        
BsiYI       BslI        BsmAI       BsrI        BstF5I      
Cfr13I      DdeI        DpnII       FokI        HinfI       
MaeI        MboI        MboII       MnlI        MseI        
MslI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      SfaNI       SfcI        TaqI        
Tru9I       Tsp509I     TspRI       TthHB8I     
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