Restriction map for pF1KB8825
##################################
# NO CUT ENZYMES (  139 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BsaAI       BsaBI       BsaWI       BseAI       BsgI        
BsiHKAI     BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstYI       BstZ17I     ClaI        CpoI        DpnII       
DraI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MamI        MboI        MfeI        MflI        
MluI        MroI        MseI        MunI        NcoI        
NdeI        NdeII       NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        Sau3AI      
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StyI        SwaI        
Tru9I       Tsp509I     Tth111I     XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI         1027  GTAGAC               GT^[AC][GT]AC
AocI         1049  CCTCAGG              CC^T[ACGT]AGG
ApaI          628  GGGCCC               GGGCC^C
AvaI         1236  CTCGGG               C^[CT]CG[GA]G
BalI         1034  TGGCCA               TGG^CCA
BbeI          887  GGCGCC               GGCGC^C
BfaI          424  CTAG                 C^TAG
BlpI          375  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      375  GCTGAGC              GC^T[ACGT]AGC
BsaI         1216  GAGACC               GGTCTC(1/5)
BsiEI         294  CGACCG               CG[GA][CT]^CG
BsmAI        1216  GAGAC                GTCTC(1/5)
BsmFI          37  GGGAC                GGGAC(10/14)
BsoBI        1236  CTCGGG               C^[CT]CG[GA]G
BsrFI         380  GCCGGC               [GA]^CCGG[CT]
BssSI         246  CTCGTG               CACGAG(-5/-1)
BstEII       1044  GGTCACC              G^GT[ACGT]ACC
BstPI        1044  GGTCACC              G^GT[ACGT]ACC
BstXI         899  CCATGCGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1049  CCTCAGG              CC^T[ACGT]AGG
CelII         375  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        380  GCCGGC               [GA]^CCGG[CT]
DrdI          475  GACGCTGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1049  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      1044  GGTCACC              G^GT[ACGT]ACC
KasI          887  GGCGCC               G^GCGCC
MaeI          424  CTAG                 C^TAG
MaeII        1031  ACGT                 A^CGT
MluNI        1034  TGGCCA               TGG^CCA
MscI         1034  TGGCCA               TGG^CCA
MslI          975  CATCCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          380  GCCGGC               GCC^GGC
NarI          887  GGCGCC               GG^CGCC
NgoMIV        380  GCCGGC               G^CCGGC
NspI         1064  ACATGC               [GA]CATG^[CT]
PflMI         849  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          793  CTGCAG               CTGCA^G
PvuII         796  CAGCTG               CAG^CTG
SapI          212  GCTCTTC              GCTCTTC(1/4)
SexAI         956  ACCTGGT              A^CC[AT]GGT
SfoI          887  GGCGCC               GGC^GCC
TaqI          293  TCGA                 T^CGA
TfiI         1201  GATTC                G^A[AT]TC
TthHB8I       293  TCGA                 T^CGA
Van91I        849  CCACATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII         357  CGCG                 CG^CG
              640  CGCG                
AcyI          178  GACGCC               G[GA]^CG[CT]C
              887  GGCGCC              
AfaI           15  GTAC                 GT^AC
              254  GTAC                
AvaII         944  GGACC                G^G[AT]CC
              984  GGACC               
BanI          523  GGTGCC               G^G[CT][GA]CC
              887  GGCGCC              
BbsI           26  GAAGAC               GAAGAC(2/6)
             1188  GTCTTC              
BpmI         1171  CTCCAG               CTGGAG(16/14)
              199  CTGGAG              
BpuAI          26  GAAGAC               GAAGAC(2/6)
             1188  GTCTTC              
BsaHI         178  GACGCC               G[GA]^CG[CT]C
              887  GGCGCC              
BseRI         325  CTCCTC               GAGGAG(10/8)
               50  GAGGAG              
BspMI         652  ACCTGC               ACCTGC(4/8)
              520  GCAGGT              
BsrBI         460  CCGCTC               CCGCTC(-3/-3)
             1120  GAGCGG              
BsrI           30  ACTGG                ACTGG(1/-1)
              486  CCAGT               
BstUI         357  CGCG                 CG^CG
              640  CGCG                
DraII         627  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              628  GGGCCCC             
DraIII        163  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              955  CACCTGGTG           
EarI          213  CTCTTC               CTCTTC(1/4)
              529  CTCTTC              
EcoO109I      627  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              628  GGGCCCC             
HgaI          178  GACGC                GACGC(5/10)
              475  GACGC               
Hin1I         178  GACGCC               G[GA]^CG[CT]C
              887  GGCGCC              
Ksp632I       213  CTCTTC               CTCTTC(1/4)
              529  CTCTTC              
MvnI          357  CGCG                 CG^CG
              640  CGCG                
PleI           85  GACTC                GAGTC(4/5)
              244  GACTC               
RsaI           15  GTAC                 GT^AC
              254  GTAC                
SfaNI        1131  GATGC                GCATC(5/9)
              572  GCATC               
XcmI          486  CCAGTGGGTCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              899  CCATGCGGCTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        EaeI        EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeIII      MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       Sau96I      ScrFI       SfcI        StuI        
TseI        Tsp45I      TspRI       
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