Restriction map for pF1KB8825
 (Linear) (Six-base) MAPSORT of: KIBB8825.seq  Check: 2373  from: 1  to: 1245



KIBB8825.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     511     661    1245 
   Size:        511     150     584

  Fragments arranged by size: 

                 584     511     150
 
AccI GT'mk_AC

Cuts at:      0    1028    1245 
   Size:       1028     217
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     264    1245 
   Size:        192      72     981

  Fragments arranged by size: 

                 981     192      72
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     799    1245 
   Size:        799     446
 
ApaI G_GGCC'C

Cuts at:      0     632    1245 
   Size:        632     613
 
AvaI C'yCGr_G

Cuts at:      0    1236    1245 
   Size:       1236       9
 
BanI G'GyrC_C

Cuts at:      0     523     887    1245 
   Size:        523     364     358

  Fragments arranged by size: 

                 523     364     358
 
BanII G_rGCy'C

Cuts at:      0     558     632     674    1172    1245 
   Size:        558      74      42     498      73

  Fragments arranged by size: 

                 558     498      74      73      42
 
BbeI G_GCGC'C

Cuts at:      0     891    1245 
   Size:        891     354
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      33    1181    1245 
   Size:         33    1148      64

  Fragments arranged by size: 

                1148      64      33
 
BlpI GC'TnA_GC

Cuts at:      0     376    1245 
   Size:        376     869
 
Bme1580I G_kGCm'C

Cuts at:      0     528     632    1245 
   Size:        528     104     613

  Fragments arranged by size: 

                 613     528     104
 
BmgBI CAC'GTC

Cuts at:      0    1032    1245 
   Size:       1032     213
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     261    1245 
   Size:        261     984
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220    1156    1245 
   Size:        220     936      89

  Fragments arranged by size: 

                 936     220      89
 
Bpu10I CC'TnA_GC

Cuts at:      0     394    1245 
   Size:        394     851
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1210    1245 
   Size:       1210      35
 
BsaHI Gr'CG_yC

Cuts at:      0     179     888    1245 
   Size:        179     709     357

  Fragments arranged by size: 

                 709     357     179
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1111    1141    1245 
   Size:       1111      30     104

  Fragments arranged by size: 

                1111     104      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      65     316    1245 
   Size:         65     251     929

  Fragments arranged by size: 

                 929     251      65
 
BseYI C'CCAG_C

Cuts at:      0     336     345     611    1245 
   Size:        336       9     266     634

  Fragments arranged by size: 

                 634     336     266       9
 
BsiEI CG_ry'CG

Cuts at:      0     297    1245 
   Size:        297     948
 
Bsp1286I G_dGCh'C

Cuts at:      0     528     558     632     674    1172    1245 
   Size:        528      30      74      42     498      73

  Fragments arranged by size: 

                 528     498      74      73      42      30
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     511     661    1245 
   Size:        511     150     584

  Fragments arranged by size: 

                 584     511     150
 
BsrBI CCG'CTC

Cuts at:      0     462    1122    1245 
   Size:        462     660     123

  Fragments arranged by size: 

                 660     462     123
 
BsrFI r'CCGG_y

Cuts at:      0     380    1245 
   Size:        380     865
 
BssSI C'ACGA_G

Cuts at:      0     246    1245 
   Size:        246     999
 
BstEII G'GTnAC_C

Cuts at:      0    1044    1245 
   Size:       1044     201
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     906    1245 
   Size:        906     339
 
Bsu36I CC'TnA_GG

Cuts at:      0    1050    1245 
   Size:       1050     195
 
BtsI GCAGTG_nn'

Cuts at:      0     454     476     744    1068    1245 
   Size:        454      22     268     324     177

  Fragments arranged by size: 

                 454     324     268     177      22
 
DraIII CAC_nnn'GTG

Cuts at:      0     168     960    1245 
   Size:        168     792     285

  Fragments arranged by size: 

                 792     285     168
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     481    1245 
   Size:        481     764
 
EaeI y'GGCC_r

Cuts at:      0     857    1011    1034    1245 
   Size:        857     154      23     211

  Fragments arranged by size: 

                 857     211     154      23
 
EarI CTCTTCn'nnn_

Cuts at:      0     219     535    1245 
   Size:        219     316     710

  Fragments arranged by size: 

                 710     316     219
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     399    1245 
   Size:        399     846
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     220     264    1156    1245 
   Size:        192      28      44     892      89

  Fragments arranged by size: 

                 892     192      89      44      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0     628     629    1245 
   Size:        628       1     616

  Fragments arranged by size: 

                 628     616       1
 
HaeII r_GCGC'y

Cuts at:      0     732     891     995    1245 
   Size:        732     159     104     250

  Fragments arranged by size: 

                 732     250     159     104
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     936     968    1245 
   Size:        936      32     277

  Fragments arranged by size: 

                 936     277      32
 
KasI G'GCGC_C

Cuts at:      0     887    1245 
   Size:        887     358
 
MscI TGG'CCA

Cuts at:      0    1036    1245 
   Size:       1036     209
 
MslI CAynn'nnrTG

Cuts at:      0     979    1245 
   Size:        979     266
 
MspA1I CmG'CkG

Cuts at:      0     798     885    1086    1245 
   Size:        798      87     201     159

  Fragments arranged by size: 

                 798     201     159      87
 
NaeI GCC'GGC

Cuts at:      0     382    1245 
   Size:        382     863
 
NarI GG'CG_CC

Cuts at:      0     888    1245 
   Size:        888     357
 
NgoMIV G'CCGG_C

Cuts at:      0     380    1245 
   Size:        380     865
 
NspI r_CATG'y

Cuts at:      0    1068    1245 
   Size:       1068     177
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     855    1245 
   Size:        855     390
 
PfoI T'CCnGG_A

Cuts at:      0     197    1115    1245 
   Size:        197     918     130

  Fragments arranged by size: 

                 918     197     130
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     196     228    1245 
   Size:        196      32    1017

  Fragments arranged by size: 

                1017     196      32
 
PspOMI G'GGCC_C

Cuts at:      0     628    1245 
   Size:        628     617
 
PstI C_TGCA'G

Cuts at:      0     797    1245 
   Size:        797     448
 
PvuII CAG'CTG

Cuts at:      0     798    1245 
   Size:        798     447
 
SapI GCTCTTCn'nnn_

Cuts at:      0     219    1245 
   Size:        219    1026
 
SexAI A'CCwGG_T

Cuts at:      0     956    1245 
   Size:        956     289
 
SfcI C'TryA_G

Cuts at:      0     318     428     793    1245 
   Size:        318     110     365     452

  Fragments arranged by size: 

                 452     365     318     110
 
SfoI GGC'GCC

Cuts at:      0     889    1245 
   Size:        889     356
 
StuI AGG'CCT

Cuts at:      0     113    1055    1228    1245 
   Size:        113     942     173      17

  Fragments arranged by size: 

                 942     173     113      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     625     757    1032    1245 
   Size:        625     132     275     213

  Fragments arranged by size: 

                 625     275     213     132
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1111    1143    1245 
   Size:       1111      32     102

  Fragments arranged by size: 

                1111     102      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     493     906    1245 
   Size:        493     413     339

  Fragments arranged by size: 

                 493     413     339
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AlfI     ApaI     AvaI     BanI    BanII
     BbeI     BbsI     BlpI Bme1580I    BmgBI     BplI     BpmI   Bpu10I
     BsaI    BsaHI    BsaXI    BseRI    BseYI    BsiEI Bsp1286I    BspMI
    BsrBI    BsrFI    BssSI   BstEII    BstXI   Bsu36I     BtsI   DraIII
     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I    HaeII    Hin4I
     KasI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
    PflMI     PfoI     PpiI   PspOMI     PstI    PvuII     SapI    SexAI
     SfcI     SfoI     StuI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BmrI     BmtI    BpuEI    BsaAI    BsaBI    BsaWI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI
    BsrGI   BssHII   BstAPI    BstBI    BstYI  BstZ17I     BtgI    BtgZI
     ClaI    CspCI     DraI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PmeI     PmlI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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