Restriction map for pF1KB8822
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       AccI        AccII       AclI        AflII       
AflIII      AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FspI        HaeII       
HincII      HindII      HpaI        KasI        KspI        
MaeI        MaeII       MamI        MfeI        MflI        
MluI        MspA1I      MunI        MvnI        NarI        
NcoI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        483  GGTACC               G^GTACC
AccIII        921  TCCGGA               T^CCGGA
AcsI          937  GAATTT               [GA]^AATT[CT]
AcyI           16  GACGCC               G[GA]^CG[CT]C
AgeI          262  ACCGGT               A^CCGGT
ApaI         1162  GGGCCC               GGGCC^C
ApoI          937  GAATTT               [GA]^AATT[CT]
Asp718I       483  GGTACC               G^GTACC
AvaI         1043  CCCGGG               C^[CT]CG[GA]G
BpmI          872  CTCCAG               CTGGAG(16/14)
BsaHI          16  GACGCC               G[GA]^CG[CT]C
BsaI          824  GAGACC               GGTCTC(1/5)
BseAI         921  TCCGGA               T^CCGGA
BsoBI        1043  CCCGGG               C^[CT]CG[GA]G
BspEI         921  TCCGGA               T^CCGGA
CfoI          258  GCGC                 GCG^C
DraI         1152  TTTAAA               TTT^AAA
DsaI          104  CCGTGG               C^C[GA][CT]GG
EarI          914  GAAGAG               CTCTTC(1/4)
EcoNI         272  CCTTCTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI          523  GGCCGGCC             GGCCGG^CC
HhaI          258  GCGC                 GCG^C
Hin1I          16  GACGCC               G[GA]^CG[CT]C
HinP1I        258  GCGC                 G^CGC
HindIII       376  AAGCTT               A^AGCTT
KpnI          483  GGTACC               GGTAC^C
Ksp632I       914  GAAGAG               CTCTTC(1/4)
MroI          921  TCCGGA               T^CCGGA
MslI          540  CATCCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          524  GCCGGC               GCC^GGC
NdeI         1139  CATATG               CA^TATG
NgoMIV        524  GCCGGC               G^CCGGC
NspI         1055  ACATGC               [GA]CATG^[CT]
PinAI         262  ACCGGT               A^CCGGT
PpuMI         744  AGGACCC              [GA]G^G[AT]CC[CT]
SacI          917  GAGCTC               GAGCT^C
SapI          914  GAAGAGC              GCTCTTC(1/4)
SgrAI         261  CACCGGTG             C[GA]^CCGG[CT]G
SmaI         1043  CCCGGG               CCC^GGG
TaqI          954  TCGA                 T^CGA
TthHB8I       954  TCGA                 T^CGA
XmaCI        1043  CCCGGG               C^CCGGG
XmaI         1043  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI          155  GTAC                 GT^AC
              484  GTAC                
BlpI          570  GCTCAGC              GC^T[ACGT]AGC
              847  GCTAAGC             
Bpu1102I      570  GCTCAGC              GC^T[ACGT]AGC
              847  GCTAAGC             
BsaWI         262  ACCGGT               [AT]^CCGG[AT]
              921  TCCGGA              
BsmAI         824  GAGAC                GTCTC(1/5)
              316  GTCTC               
BsmFI         348  GGGAC                GGGAC(10/14)
              806  GGGAC               
BsrDI         363  CATTGC               GCAATG(2/0)
              110  GCAATG              
BsrFI         262  ACCGGT               [GA]^CCGG[CT]
              524  GCCGGC              
CelII         570  GCTCAGC              GC^T[ACGT]AGC
              847  GCTAAGC             
Cfr10I        262  ACCGGT               [GA]^CCGG[CT]
              524  GCCGGC              
DpnII         196  GATC                 ^GATC
              831  GATC                
EcoT14I       131  CCTTGG               C^C[AT][AT]GG
              401  CCAAGG              
HgaI           16  GACGC                GACGC(5/10)
              350  GACGC               
MaeIII         94  GTGAC                ^GT[ACGT]AC
              949  GTGAC               
MboI          196  GATC                 ^GATC
              831  GATC                
MseI          902  TTAA                 T^TAA
             1153  TTAA                
NdeII         196  GATC                 ^GATC
              831  GATC                
PflMI         714  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              792  CCAGACCCTGG         
PstI          276  CTGCAG               CTGCA^G
              501  CTGCAG              
RsaI          155  GTAC                 GT^AC
              484  GTAC                
Sau3AI        196  GATC                 ^GATC
              831  GATC                
StyI          131  CCTTGG               C^C[AT][AT]GG
              401  CCAAGG              
TfiI          161  GATTC                G^A[AT]TC
              925  GATTC               
Tru9I         902  TTAA                 T^TAA
             1153  TTAA                
Tsp45I         94  GTGAC                ^GT[GC]AC
              949  GTGAC               
Van91I        714  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              792  CCAGACCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AluI        AlwNI       AspHI       AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BmyI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstXI       Cac8I       
Cfr13I      DdeI        DraII       EaeI        EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MboII       
MluNI       MnlI        MscI        MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       PleI        
Sau96I      ScrFI       SfaNI       SfcI        TseI        
Tsp509I     TspRI       XcmI        
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