Restriction map for pF1KB8811
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
KspI        MaeI        MaeIII      MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfcI        
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TfiI        Tru9I       Tsp45I      Tsp509I     
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI          160  GTCTAC               GT^[AC][GT]AC
AflIII         11  ACACGT               A^C[GA][CT]GT
AvaI          259  CCCGGG               C^[CT]CG[GA]G
BamHI         248  GGATCC               G^GATCC
BglI          442  GCCAACTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          967  CTGGAG               CTGGAG(16/14)
BseRI         781  GAGGAG               GAGGAG(10/8)
BsoBI         259  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      194  TGTACA               T^GTACA
BsrGI         194  TGTACA               T^GTACA
BstAPI        903  GCACAACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         248  GGATCC               [GA]^GATC[CT]
EcoNI         983  CCTCCAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII        865  GTCAAC               GT[CT]^[GA]AC
HindII        865  GTCAAC               GT[CT]^[GA]AC
HinfI          34  GACTC                G^A[ACGT]TC
MflI          248  GGATCC               [GA]^GATC[CT]
NcoI          518  CCATGG               C^CATGG
PflMI         939  CCAGGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           34  GACTC                GAGTC(4/5)
PpuMI         262  GGGTCCT              [GA]G^G[AT]CC[CT]
SapI          471  GCTCTTC              GCTCTTC(1/4)
SexAI        1052  ACCAGGT              A^CC[AT]GGT
SmaI          259  CCCGGG               CCC^GGG
SspBI         194  TGTACA               T^GTACA
TaqI          779  TCGA                 T^CGA
TspRI         812  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       779  TCGA                 T^CGA
Van91I        939  CCAGGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           98  CCACCAACTGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         248  GGATCC               [GA]^GATC[CT]
XmaCI         259  CCCGGG               C^CCGGG
XmaI          259  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          195  GTAC                 GT^AC
              600  GTAC                
AlwI          249  GATCC                GGATC(4/5)
              248  GGATC               
BanII         411  GAGCTC               G[GA]GC[CT]^C
              469  GAGCTC              
BbvCI         174  CCTCAGC              CCTCAGC(-5/-2)
              678  CCTCAGC             
BsaI          479  GAGACC               GGTCTC(1/5)
              970  GAGACC              
BsmAI         479  GAGAC                GTCTC(1/5)
              970  GAGAC               
BspMI         188  ACCTGC               ACCTGC(4/8)
              908  ACCTGC              
DdeI          175  CTCAG                C^T[ACGT]AG
              679  CTCAG               
DpnII         249  GATC                 ^GATC
              663  GATC                
DraII         262  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              896  AGGCCCT             
EarI          472  CTCTTC               CTCTTC(1/4)
              571  CTCTTC              
EcoO109I      262  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              896  AGGCCCT             
EcoT14I       518  CCATGG               C^C[AT][AT]GG
              893  CCAAGG              
HapII         260  CCGG                 C^CGG
              603  CCGG                
HpaII         260  CCGG                 C^CGG
              603  CCGG                
Ksp632I       472  CTCTTC               CTCTTC(1/4)
              571  CTCTTC              
MboI          249  GATC                 ^GATC
              663  GATC                
MslI           81  CATCGGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              330  CATCTCGGTG          
MspI          260  CCGG                 C^CGG
              603  CCGG                
NdeII         249  GATC                 ^GATC
              663  GATC                
PvuII        1020  CAGCTG               CAG^CTG
             1060  CAGCTG              
RsaI          195  GTAC                 GT^AC
              600  GTAC                
SacI          411  GAGCTC               GAGCT^C
              469  GAGCTC              
Sau3AI        249  GATC                 ^GATC
              663  GATC                
StyI          518  CCATGG               C^C[AT][AT]GG
              893  CCAAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AspHI       
AvaII       BalI        BanI        BbeI        BbrPI       
BbvI        BcnI        BmyI        BsaAI       BsaHI       
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr13I      DsaI        EaeI        EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HgaI        
HhaI        Hin1I       HinP1I      HphI        ItaI        
KasI        MaeII       MboII       MluNI       MnlI        
MscI        MspA1I      MvaI        MvnI        MwoI        
NarI        NciI        NlaIII      NlaIV       PmaCI       
PmlI        Sau96I      ScrFI       SfaNI       SfoI        
TseI        
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