Restriction map for pF1KB8809
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BcnI        
BfrI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HapII       
HgaI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        HpaII       KasI        KpnI        
KspI        MaeIII      MluI        MluNI       MroI        
MscI        MspA1I      MspI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsrII       SacII       SalI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        TfiI        Tsp45I      
Tth111I     XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII         423  CGCG                 CG^CG
Alw44I        418  GTGCAC               G^TGCAC
AlwNI         488  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          641  CCTTAGG              CC^T[ACGT]AGG
ApaLI         418  GTGCAC               G^TGCAC
Asp700I       811  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI          71  GTATCC               GTATCC(6/5)
BclI          671  TGATCA               T^GATCA
BglI          853  GCCATCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        1057  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          793  GAGACC               GGTCTC(1/5)
BseRI        1000  CTCCTC               GAGGAG(10/8)
BsgI          825  CTGCAC               GTGCAG(16/14)
BsmAI         793  GAGAC                GTCTC(1/5)
BstUI         423  CGCG                 CG^CG
BstXI         987  CCAATACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         252  GGATCT               [GA]^GATC[CT]
Bsu36I        641  CCTTAGG              CC^T[ACGT]AGG
Cac8I         420  GCACGC               GC[ACGT]^[ACGT]GC
CfoI          543  GCGC                 GCG^C
Eco81I        641  CCTTAGG              CC^T[ACGT]AGG
FbaI          671  TGATCA               T^GATCA
HhaI          543  GCGC                 GCG^C
HinP1I        543  GCGC                 G^CGC
MaeII         589  ACGT                 A^CGT
MamI         1057  GATCATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          413  CAATTG               C^AATTG
MflI          252  GGATCT               [GA]^GATC[CT]
MseI          896  TTAA                 T^TAA
MslI          384  CATGCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          413  CAATTG               C^AATTG
MvnI          423  CGCG                 CG^CG
NcoI         1052  CCATGG               C^CATGG
PflMI         584  CCACGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          963  CTGCAG               CTGCA^G
SacI          115  GAGCTC               GAGCT^C
SbfI          962  CCTGCAGG             CCTGCA^GG
ScaI          569  AGTACT               AGT^ACT
SmlI          435  CTTGAG               C^T[CT][GA]AG
Sse8387I      962  CCTGCAGG             CCTGCA^GG
TaqI           32  TCGA                 T^CGA
Tru9I         896  TTAA                 T^TAA
TthHB8I        32  TCGA                 T^CGA
Van91I        584  CCACGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         252  GGATCT               [GA]^GATC[CT]
XmnI          811  GAAGTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BanII         115  GAGCTC               G[GA]GC[CT]^C
              997  GGGCTC              
BspHI         389  TCATGA               T^CATGA
             1059  TCATGA              
BspMI         230  ACCTGC               ACCTGC(4/8)
              961  ACCTGC              
BsrI          303  CCAGT                ACTGG(1/-1)
              470  CCAGT               
DraIII        421  CACGCGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              585  CACGACGTG           
HaeIII        861  GGCC                 GG^CC
              970  GGCC                
NlaIV         968  GGGGCC               GG[ACGT]^[ACGT]CC
              998  GGCTCC              
PpuMI         445  AGGACCT              [GA]G^G[AT]CC[CT]
              679  AGGACCT             
RcaI          389  TCATGA               T^CATGA
             1059  TCATGA              
SfaNI        1072  GATGC                GCATC(5/9)
               98  GCATC               
SfcI          946  CTACAG               C^T[GA][CT]AG
              963  CTGCAG              
XcmI          303  CCAGTGGGTTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              863  CCACAGAAACTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BbvI        BfaI        
BmyI        BsaJI       BsiHKAI     BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       DraII       DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HphI        ItaI        Ksp632I     MaeI        MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      RsaI        SapI        Sau3AI      Sau96I      
ScrFI       StyI        TseI        Tsp509I     TspRI       
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