Restriction map for pF1KB8808
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
AspEI       AspI        AvaI        AviII       BalI        
BamHI       BanII       BbeI        BbrPI       BcgI        
BciVI       BclI        BcnI        BfrI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       BstBI       BstEII      BstPI       
BstUI       Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       KasI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        TaqI        
TfiI        Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I        392  GGTACC               G^GTACC
AciI          479  CCGC                 CCGC(-3/-1)
AfaI          393  GTAC                 GT^AC
AlwI          624  GATCC                GGATC(4/5)
Asp718I       392  GGTACC               G^GTACC
AspHI         718  GTGCTC               G[AT]GC[AT]^C
BanI          392  GGTACC               G^G[CT][GA]CC
BbvCI         195  GCTGAGG              CCTCAGC(-5/-2)
BglI          481  GCCATTATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         584  AGATCT               A^GATCT
BpmI         1044  CTCCAG               CTGGAG(16/14)
BsgI          664  CTGCAC               GTGCAG(16/14)
BsiHKAI       718  GTGCTC               G[AT]GC[AT]^C
BspHI         377  TCATGA               T^CATGA
BspMI         242  ACCTGC               ACCTGC(4/8)
BsrI          291  CCAGT                ACTGG(1/-1)
Bst1107I      214  GTATAC               GTA^TAC
BstAPI        789  GCACAGTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         707  CCATCAGGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         584  AGATCT               [GA]^GATC[CT]
BstZ17I       214  GTATAC               GTA^TAC
Cac8I        1022  GCCAGC               GC[ACGT]^[ACGT]GC
DraII         752  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EarI          675  GAAGAG               CTCTTC(1/4)
EcoO109I      752  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRV          55  GATATC               GAT^ATC
KpnI          392  GGTACC               GGTAC^C
Ksp632I       675  GAAGAG               CTCTTC(1/4)
MaeII         764  ACGT                 A^CGT
MflI          584  AGATCT               [GA]^GATC[CT]
MseI          656  TTAA                 T^TAA
NspI          359  GCATGT               [GA]CATG^[CT]
PpuMI         752  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI          377  TCATGA               T^CATGA
RsaI          393  GTAC                 GT^AC
SmlI           63  CTCAAG               C^T[CT][GA]AG
SspI          939  AATATT               AAT^ATT
Tru9I         656  TTAA                 T^TAA
XcmI          291  CCAGTGGGTGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         584  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AccI          214  GTATAC               GT^[AC][GT]AC
             1051  GTAGAC              
AvrII         975  CCTAGG               C^CTAGG
              991  CCTAGG              
BlnI          975  CCTAGG               C^CTAGG
              991  CCTAGG              
BmyI          447  GGGCAC               G[AGT]GC[ACT]^C
              718  GTGCTC              
BseRI        1032  CTCCTC               GAGGAG(10/8)
              198  GAGGAG              
BsiYI          70  CCCTGTGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               71  CCTGTGATGGG         
BslI           70  CCCTGTGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               71  CCTGTGATGGG         
BsmFI         304  GGGAC                GGGAC(10/14)
              754  GTCCC               
Bsp1286I      447  GGGCAC               G[AGT]GC[ACT]^C
              718  GTGCTC              
BsrDI         500  CATTGC               GCAATG(2/0)
              732  CATTGC              
MspA1I        670  CAGCTG               C[AC]G^C[GT]G
              829  CAGCTG              
PvuII         670  CAGCTG               CAG^CTG
              829  CAGCTG              
SfaNI         203  GCATC                GCATC(5/9)
              736  GCATC               
SfcI          351  CTACAG               C^T[GA][CT]AG
              541  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AluI        Asp700I     AvaII       BbsI        BbvI        
BfaI        BpuAI       BsaJI       BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MnlI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
StyI        TseI        Tsp45I      TspRI       XmnI        
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