Restriction map for
pF1KB8797
(Linear) (Six-base) MAPSORT of: KIBB8797.seq Check: 6573 from: 1 to: 999
KIBB8797.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 257 978 999
Size: 257 721 21
Fragments arranged by size:
721 257 21
AflIII A'CryG_T
Cuts at: 0 188 437 999
Size: 188 249 562
Fragments arranged by size:
562 249 188
AleI CACnn'nnGTG
Cuts at: 0 571 999
Size: 571 428
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 196 999
Size: 196 803
AlwNI CAG_nnn'CTG
Cuts at: 0 63 556 993 999
Size: 63 493 437 6
Fragments arranged by size:
493 437 63 6
ApaLI G'TGCA_C
Cuts at: 0 244 392 717 999
Size: 244 148 325 282
Fragments arranged by size:
325 282 244 148
AvaI C'yCGr_G
Cuts at: 0 261 999
Size: 261 738
BanI G'GyrC_C
Cuts at: 0 591 999
Size: 591 408
BanII G_rGCy'C
Cuts at: 0 268 686 734 999
Size: 268 418 48 265
Fragments arranged by size:
418 268 265 48
BlpI GC'TnA_GC
Cuts at: 0 226 367 999
Size: 226 141 632
Fragments arranged by size:
632 226 141
Bme1580I G_kGCm'C
Cuts at: 0 248 396 721 767 999
Size: 248 148 325 46 232
Fragments arranged by size:
325 248 232 148 46
BmgBI CAC'GTC
Cuts at: 0 189 999
Size: 189 810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 843 999
Size: 700 143 156
Fragments arranged by size:
700 156 143
BsaAI yAC'GTr
Cuts at: 0 306 438 588 999
Size: 306 132 150 411
Fragments arranged by size:
411 306 150 132
BsaHI Gr'CG_yC
Cuts at: 0 563 739 999
Size: 563 176 260
Fragments arranged by size:
563 260 176
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 471 999
Size: 55 416 528
Fragments arranged by size:
528 416 55
BseYI C'CCAG_C
Cuts at: 0 69 93 759 910 999
Size: 69 24 666 151 89
Fragments arranged by size:
666 151 89 69 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 999
Size: 805 194
BsiHKAI G_wGCw'C
Cuts at: 0 227 248 268 396 721 999
Size: 227 21 20 128 325 278
Fragments arranged by size:
325 278 227 128 21 20
Bsp1286I G_dGCh'C
Cuts at: 0 227 248 268 396 686 721 734 767
Size: 227 21 20 128 290 35 13 33
Cuts at: 767 999
Size: 232
Fragments arranged by size:
290 232 227 128 35 33 21 20
13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 71 257 978 999
Size: 71 186 721 21
Fragments arranged by size:
721 186 71 21
BsrFI r'CCGG_y
Cuts at: 0 674 953 999
Size: 674 279 46
Fragments arranged by size:
674 279 46
BsrGI T'GTAC_A
Cuts at: 0 422 999
Size: 422 577
BssHII G'CGCG_C
Cuts at: 0 461 521 999
Size: 461 60 478
Fragments arranged by size:
478 461 60
BstAPI GCAn_nnn'nTGC
Cuts at: 0 556 990 999
Size: 556 434 9
Fragments arranged by size:
556 434 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 849 999
Size: 849 150
BstYI r'GATC_y
Cuts at: 0 317 999
Size: 317 682
BtgI C'CryG_G
Cuts at: 0 400 794 999
Size: 400 394 205
Fragments arranged by size:
400 394 205
BtsI GCAGTG_nn'
Cuts at: 0 215 310 394 957 999
Size: 215 95 84 563 42
Fragments arranged by size:
563 215 95 84 42
DraIII CAC_nnn'GTG
Cuts at: 0 716 828 999
Size: 716 112 171
Fragments arranged by size:
716 171 112
EaeI y'GGCC_r
Cuts at: 0 149 344 413 602 707 999
Size: 149 195 69 189 105 292
Fragments arranged by size:
292 195 189 149 105 69
EarI CTCTTCn'nnn_
Cuts at: 0 496 999
Size: 496 503
EcoICRI GAG'CTC
Cuts at: 0 266 999
Size: 266 733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 700 843 999
Size: 700 143 156
Fragments arranged by size:
700 156 143
EcoO109I rG'GnC_Cy
Cuts at: 0 60 999
Size: 60 939
HaeII r_GCGC'y
Cuts at: 0 233 280 999
Size: 233 47 719
Fragments arranged by size:
719 233 47
HincII GTy'rAC
Cuts at: 0 201 999
Size: 201 798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 999
Size: 945 54
MscI TGG'CCA
Cuts at: 0 151 346 415 604 709 999
Size: 151 195 69 189 105 290
Fragments arranged by size:
290 195 189 151 105 69
MslI CAynn'nnrTG
Cuts at: 0 178 208 571 979 999
Size: 178 30 363 408 20
Fragments arranged by size:
408 363 178 30 20
MspA1I CmG'CkG
Cuts at: 0 14 69 402 999
Size: 14 55 333 597
Fragments arranged by size:
597 333 55 14
NaeI GCC'GGC
Cuts at: 0 676 999
Size: 676 323
NgoMIV G'CCGG_C
Cuts at: 0 674 999
Size: 674 325
NruI TCG'CGA
Cuts at: 0 783 999
Size: 783 216
PmlI CAC'GTG
Cuts at: 0 306 438 999
Size: 306 132 561
Fragments arranged by size:
561 306 132
PpuMI rG'GwC_Cy
Cuts at: 0 60 999
Size: 60 939
PstI C_TGCA'G
Cuts at: 0 13 68 317 505 999
Size: 13 55 249 188 494
Fragments arranged by size:
494 249 188 55 13
PvuII CAG'CTG
Cuts at: 0 14 69 999
Size: 14 55 930
Fragments arranged by size:
930 55 14
SacI G_AGCT'C
Cuts at: 0 268 999
Size: 268 731
SacII CC_GC'GG
Cuts at: 0 403 999
Size: 403 596
SapI GCTCTTCn'nnn_
Cuts at: 0 496 999
Size: 496 503
SfcI C'TryA_G
Cuts at: 0 9 64 313 501 999
Size: 9 55 249 188 498
Fragments arranged by size:
498 249 188 55 9
StuI AGG'CCT
Cuts at: 0 218 999
Size: 218 781
TatI w'GTAC_w
Cuts at: 0 191 350 422 999
Size: 191 159 72 577
Fragments arranged by size:
577 191 159 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 302 334 999
Size: 302 32 665
Fragments arranged by size:
665 302 32
XmnI GAAnn'nnTTC
Cuts at: 0 835 999
Size: 835 164
Enzymes that do cut:
AarI AflIII AleI AlfI AlwNI ApaLI AvaI BanI
BanII BlpI Bme1580I BmgBI BpmI BsaAI BsaHI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BsrGI BssHII
BstAPI BstXI BstYI BtgI BtsI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I HaeII HincII MmeI MscI MslI
MspA1I NaeI NgoMIV NruI PmlI PpuMI PstI PvuII
SacI SacII SapI SfcI StuI TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StyI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI ZraI