Restriction map for pF1KB8796
 (Linear) (Six-base) MAPSORT of: KIBB8796.seq  Check: 9935  from: 1  to: 999



KIBB8796.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AccI GT'mk_AC

Cuts at:      0     560     999 
   Size:        560     439
 
Acc65I G'GTAC_C

Cuts at:      0     545     999 
   Size:        545     454
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     999 
   Size:         34     965
 
AflIII A'CryG_T

Cuts at:      0     897     999 
   Size:        897     102
 
AlwNI CAG_nnn'CTG

Cuts at:      0     747     999 
   Size:        747     252
 
BanI G'GyrC_C

Cuts at:      0     545     656     933     967     985     999 
   Size:        545     111     277      34      18      14

  Fragments arranged by size: 

                 545     277     111      34      18      14
 
BanII G_rGCy'C

Cuts at:      0     400     999 
   Size:        400     599
 
BbeI G_GCGC'C

Cuts at:      0     660     989     999 
   Size:        660     329      10

  Fragments arranged by size: 

                 660     329      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     855     999 
   Size:        855     144
 
BbvCI CC'TCA_GC

Cuts at:      0     130     706     999 
   Size:        130     576     293

  Fragments arranged by size: 

                 576     293     130
 
BglI GCCn_nnn'nGGC

Cuts at:      0     140     999 
   Size:        140     859
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     285     999 
   Size:        285     714
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     316     999 
   Size:        316     683
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     999 
   Size:        205     794
 
Bpu10I CC'TnA_GC

Cuts at:      0     130     706     999 
   Size:        130     576     293

  Fragments arranged by size: 

                 576     293     130
 
BsaAI yAC'GTr

Cuts at:      0     542     615     999 
   Size:        542      73     384

  Fragments arranged by size: 

                 542     384      73
 
BsaHI Gr'CG_yC

Cuts at:      0     657     986     999 
   Size:        657     329      13

  Fragments arranged by size: 

                 657     329      13
 
BsaWI w'CCGG_w

Cuts at:      0     767     999 
   Size:        767     232
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     538     568     887     917     999 
   Size:        538      30     319      30      82

  Fragments arranged by size: 

                 538     319      82      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118     301     999 
   Size:        118     183     698

  Fragments arranged by size: 

                 698     183     118
 
BseYI C'CCAG_C

Cuts at:      0     281     288     710     937     942     999 
   Size:        281       7     422     227       5      57

  Fragments arranged by size: 

                 422     281     227      57       7       5
 
BsiHKAI G_wGCw'C

Cuts at:      0      28     857     999 
   Size:         28     829     142

  Fragments arranged by size: 

                 829     142      28
 
Bsp1286I G_dGCh'C

Cuts at:      0      28     400     857     999 
   Size:         28     372     457     142

  Fragments arranged by size: 

                 457     372     142      28
 
BspEI T'CCGG_A

Cuts at:      0     767     999 
   Size:        767     232
 
BspHI T'CATG_A

Cuts at:      0     365     999 
   Size:        365     634
 
BsrBI CCG'CTC

Cuts at:      0     641     999 
   Size:        641     358
 
BsrFI r'CCGG_y

Cuts at:      0     923     999 
   Size:        923      76
 
BstEII G'GTnAC_C

Cuts at:      0     444     999 
   Size:        444     555
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     275     693     964     999 
   Size:        275     418     271      35

  Fragments arranged by size: 

                 418     275     271      35
 
BstYI r'GATC_y

Cuts at:      0     260     620     999 
   Size:        260     360     379

  Fragments arranged by size: 

                 379     360     260
 
Bsu36I CC'TnA_GG

Cuts at:      0       9     625     999 
   Size:          9     616     374

  Fragments arranged by size: 

                 616     374       9
 
BtgI C'CryG_G

Cuts at:      0     449     999 
   Size:        449     550
 
BtsI GCAGTG_nn'

Cuts at:      0     834     999 
   Size:        834     165
 
DraIII CAC_nnn'GTG

Cuts at:      0     507     999 
   Size:        507     492
 
EarI CTCTTCn'nnn_

Cuts at:      0     580     999 
   Size:        580     419
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      34     205     999 
   Size:         34     171     794

  Fragments arranged by size: 

                 794     171      34
 
EcoO109I rG'GnC_Cy

Cuts at:      0     162     629     715     999 
   Size:        162     467      86     284

  Fragments arranged by size: 

                 467     284     162      86
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     696     999 
   Size:        696     303
 
HaeII r_GCGC'y

Cuts at:      0     660     989     999 
   Size:        660     329      10

  Fragments arranged by size: 

                 660     329      10
 
KasI G'GCGC_C

Cuts at:      0     656     985     999 
   Size:        656     329      14

  Fragments arranged by size: 

                 656     329      14
 
KpnI G_GTAC'C

Cuts at:      0     549     999 
   Size:        549     450
 
MslI CAynn'nnrTG

Cuts at:      0     454     691     999 
   Size:        454     237     308

  Fragments arranged by size: 

                 454     308     237
 
MspA1I CmG'CkG

Cuts at:      0     431     710     999 
   Size:        431     279     289

  Fragments arranged by size: 

                 431     289     279
 
NaeI GCC'GGC

Cuts at:      0     925     999 
   Size:        925      74
 
NarI GG'CG_CC

Cuts at:      0     657     986     999 
   Size:        657     329      13

  Fragments arranged by size: 

                 657     329      13
 
NcoI C'CATG_G

Cuts at:      0     449     999 
   Size:        449     550
 
NgoMIV G'CCGG_C

Cuts at:      0     923     999 
   Size:        923      76
 
NspI r_CATG'y

Cuts at:      0     901     999 
   Size:        901      98
 
PasI CC'CwG_GG

Cuts at:      0     591     999 
   Size:        591     408
 
PciI A'CATG_T

Cuts at:      0     897     999 
   Size:        897     102
 
PmlI CAC'GTG

Cuts at:      0     542     999 
   Size:        542     457
 
PpuMI rG'GwC_Cy

Cuts at:      0     162     629     715     999 
   Size:        162     467      86     284

  Fragments arranged by size: 

                 467     284     162      86
 
PstI C_TGCA'G

Cuts at:      0     743     932     999 
   Size:        743     189      67

  Fragments arranged by size: 

                 743     189      67
 
PvuII CAG'CTG

Cuts at:      0     710     999 
   Size:        710     289
 
SanDI GG'GwC_CC

Cuts at:      0     715     999 
   Size:        715     284
 
SexAI A'CCwGG_T

Cuts at:      0     230     999 
   Size:        230     769
 
SfcI C'TryA_G

Cuts at:      0     339     739     928     999 
   Size:        339     400     189      71

  Fragments arranged by size: 

                 400     339     189      71
 
SfoI GGC'GCC

Cuts at:      0     658     987     999 
   Size:        658     329      12

  Fragments arranged by size: 

                 658     329      12
 
StuI AGG'CCT

Cuts at:      0      76     999 
   Size:         76     923
 
StyI C'CwwG_G

Cuts at:      0     449     999 
   Size:        449     550
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     273     951     999 
   Size:        273     678      48

  Fragments arranged by size: 

                 678     273      48
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII    AlwNI     BanI    BanII     BbeI
     BbsI    BbvCI     BglI     BmrI     BplI     BpmI   Bpu10I    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI   BstEII    BstXI    BstYI   Bsu36I     BtgI
     BtsI   DraIII     EarI  Eco57MI EcoO109I     FalI    HaeII     KasI
     KpnI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PasI     PciI     PmlI    PpuMI     PstI    PvuII    SanDI    SexAI
     SfcI     SfoI     StuI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmtI    BpuEI     BsaI    BsaBI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspMI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI  BstZ17I    BtgZI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     MfeI     MluI     MmeI     MscI     NdeI     NheI     NotI     NruI
     NsiI     PacI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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