(Circular) (Six-base) MAPSORT of: pF1KB8788.seq Check: 7210 from: 1 to: 4013 pF1KB8788 4013 bp With 182 enzymes: SgfI * June 20, 2008 10:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 346 346 Size: 4013 AccI GT'mk_AC Cuts at: 1051 1051 Size: 4013 Acc65I G'GTAC_C Cuts at: 1029 1029 Size: 4013 AclI AA'CG_TT Cuts at: 3853 3853 Size: 4013 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1838 2270 3017 3285 1838 Size: 432 747 268 2566 Fragments arranged by size: 2566 747 432 268 AflIII A'CryG_T Cuts at: 1365 2469 3446 1365 Size: 1104 977 1932 Fragments arranged by size: 1932 1104 977 AgeI A'CCGG_T Cuts at: 2401 2401 Size: 4013 AlwNI CAG_nnn'CTG Cuts at: 356 1318 2885 356 Size: 962 1567 1484 Fragments arranged by size: 1567 1484 962 ApaI G_GGCC'C Cuts at: 383 383 Size: 4013 ApaLI G'TGCA_C Cuts at: 2783 2783 Size: 4013 ApoI r'AATT_y Cuts at: 1017 3366 3622 1017 Size: 2349 256 1408 Fragments arranged by size: 2349 1408 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4013 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4013 AvaI C'yCGr_G Cuts at: 260 1033 260 Size: 773 3240 BamHI G'GATC_C Cuts at: 1038 1038 Size: 4013 BanI G'GyrC_C Cuts at: 1029 1443 1691 1726 1029 Size: 414 248 35 3316 Fragments arranged by size: 3316 414 248 35 BanII G_rGCy'C Cuts at: 308 383 1027 2057 308 Size: 75 644 1030 2264 Fragments arranged by size: 2264 1030 644 75 BbeI G_GCGC'C Cuts at: 1447 1695 1447 Size: 248 3765 BciVI GTATCCnnnnn_n' Cuts at: 1911 2672 4013 1911 Size: 761 1341 1911 Fragments arranged by size: 1911 1341 761 BclI T'GATC_A Cuts at: 449 1533 449 Size: 1084 2929 BglII A'GATC_T Cuts at: 1528 3565 1528 Size: 2037 1976 BlpI GC'TnA_GC Cuts at: 1125 1125 Size: 4013 Bme1580I G_kGCm'C Cuts at: 383 1638 1731 2787 383 Size: 1255 93 1056 1609 Fragments arranged by size: 1609 1255 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1225 1306 1641 3785 1225 Size: 81 335 2144 1453 Fragments arranged by size: 2144 1453 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 144 2380 144 Size: 2236 1777 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 284 284 Size: 4013 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1189 2068 2560 2858 1189 Size: 879 492 298 2344 Fragments arranged by size: 2344 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 272 3717 272 Size: 3445 568 BsaAI yAC'GTr Cuts at: 1366 1366 Size: 4013 BsaBI GATnn'nnATC Cuts at: 6 1527 3564 6 Size: 1521 2037 455 Fragments arranged by size: 2037 1521 455 BsaHI Gr'CG_yC Cuts at: 1444 1692 2394 3890 1444 Size: 248 702 1496 1567 Fragments arranged by size: 1567 1496 702 248 BsaWI w'CCGG_w Cuts at: 1202 1723 2401 2675 2822 3233 1202 Size: 521 678 274 147 411 1982 Fragments arranged by size: 1982 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 357 439 442 357 Size: 82 3 3928 Fragments arranged by size: 3928 82 3 BseYI C'CCAG_C Cuts at: 408 1438 2196 2773 3269 408 Size: 1030 758 577 496 1152 Fragments arranged by size: 1152 1030 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 673 706 673 Size: 33 3980 BsiEI CG_ry'CG Cuts at: 104 1555 1601 2809 104 Size: 1451 46 1208 1308 Fragments arranged by size: 1451 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1027 1805 1995 2787 1027 Size: 778 190 792 2253 Fragments arranged by size: 2253 792 778 190 Bsp1286I G_dGCh'C Cuts at: 308 383 1027 1638 1731 1805 1995 2057 2787 Size: 75 644 611 93 74 190 62 730 Cuts at: 2787 308 Size: 1534 Fragments arranged by size: 1534 730 644 611 190 93 75 74 62 BspEI T'CCGG_A Cuts at: 3233 3233 Size: 4013 BspHI T'CATG_A Cuts at: 3189 3189 Size: 4013 BspMI ACCTGCnnnn'nnnn_ Cuts at: 346 1063 1277 1579 1960 346 Size: 717 214 302 381 2399 Fragments arranged by size: 2399 717 381 302 214 BsrBI CCG'CTC Cuts at: 50 128 2305 2359 3843 4008 50 Size: 78 2177 54 1484 165 55 Fragments arranged by size: 2177 1484 165 78 55 54 BsrDI GCAATG_nn' Cuts at: 1925 1925 Size: 4013 BsrFI r'CCGG_y Cuts at: 2011 2192 2401 2011 Size: 181 209 3623 Fragments arranged by size: 3623 209 181 BssSI C'ACGA_G Cuts at: 2284 2642 2284 Size: 358 3655 BstAPI GCAn_nnn'nTGC Cuts at: 1390 1390 Size: 4013 BstBI TT'CG_AA Cuts at: 2374 2374 Size: 4013 BstYI r'GATC_y Cuts at: 1 1038 1505 1528 1863 2109 3121 3207 3219 Size: 1037 467 23 335 246 1012 86 12 Cuts at: 3219 3565 1 Size: 346 449 Fragments arranged by size: 1037 1012 467 449 346 335 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 133 133 Size: 4013 BtgI C'CryG_G Cuts at: 107 107 Size: 4013 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1963 2144 2307 1963 Size: 181 163 3669 Fragments arranged by size: 3669 181 163 BtsI GCAGTG_nn' Cuts at: 694 1256 694 Size: 562 3451 DraI TTT'AAA Cuts at: 927 1012 927 Size: 85 3928 DrdI GACnn_nn'nnGTC Cuts at: 1719 2577 1719 Size: 858 3155 EaeI y'GGCC_r Cuts at: 1598 1772 2163 2190 3535 1598 Size: 174 391 27 1345 2076 Fragments arranged by size: 2076 1345 391 174 27 EagI C'GGCC_G Cuts at: 1598 1598 Size: 4013 EarI CTCTTCn'nnn_ Cuts at: 506 752 809 2036 2246 506 Size: 246 57 1227 210 2273 Fragments arranged by size: 2273 1227 246 210 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2221 2531 2677 3823 2221 Size: 310 146 1146 2411 Fragments arranged by size: 2411 1146 310 146 EcoICRI GAG'CTC Cuts at: 1025 1025 Size: 4013 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 284 1838 2270 3017 3285 284 Size: 1554 432 747 268 1012 Fragments arranged by size: 1554 1012 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 196 196 Size: 4013 EcoO109I rG'GnC_Cy Cuts at: 379 380 1152 379 Size: 1 772 3240 Fragments arranged by size: 3240 772 1 EcoRI G'AATT_C Cuts at: 1017 1017 Size: 4013 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 477 2032 477 Size: 1555 2458 FspI TGC'GCA Cuts at: 1794 1794 Size: 4013 HaeII r_GCGC'y Cuts at: 1447 1695 2717 3696 1447 Size: 248 1022 979 1764 Fragments arranged by size: 1764 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 188 220 1855 1885 1887 1917 188 Size: 32 1635 30 2 30 2284 Fragments arranged by size: 2284 1635 32 30 30 2 HincII GTy'rAC Cuts at: 1052 1052 Size: 4013 KasI G'GCGC_C Cuts at: 1443 1691 1443 Size: 248 3765 KpnI G_GTAC'C Cuts at: 1033 1033 Size: 4013 MluI A'CGCG_T Cuts at: 3446 3446 Size: 4013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 497 2684 2868 497 Size: 2187 184 1642 Fragments arranged by size: 2187 1642 184 MscI TGG'CCA Cuts at: 1774 1774 Size: 4013 MslI CAynn'nnrTG Cuts at: 2002 2129 2002 Size: 127 3886 MspA1I CmG'CkG Cuts at: 356 596 1124 1438 1798 2811 3056 356 Size: 240 528 314 360 1013 245 1313 Fragments arranged by size: 1313 1013 528 360 314 245 240 NaeI GCC'GGC Cuts at: 2194 2194 Size: 4013 NarI GG'CG_CC Cuts at: 1444 1692 1444 Size: 248 3765 NcoI C'CATG_G Cuts at: 107 107 Size: 4013 NgoMIV G'CCGG_C Cuts at: 2192 2192 Size: 4013 NspI r_CATG'y Cuts at: 1066 1566 2473 1066 Size: 500 907 2606 Fragments arranged by size: 2606 907 500 PasI CC'CwG_GG Cuts at: 376 376 Size: 4013 PciI A'CATG_T Cuts at: 2469 2469 Size: 4013 PmeI GTTT'AAAC Cuts at: 1012 1012 Size: 4013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3152 3184 3152 Size: 32 3981 PspOMI G'GGCC_C Cuts at: 379 379 Size: 4013 PstI C_TGCA'G Cuts at: 1060 1745 1060 Size: 685 3328 PvuI CG_AT'CG Cuts at: 104 104 Size: 4013 PvuII CAG'CTG Cuts at: 1438 1798 1438 Size: 360 3653 RsrII CG'GwC_CG Cuts at: 2208 2208 Size: 4013 SacI G_AGCT'C Cuts at: 1027 1027 Size: 4013 SalI G'TCGA_C Cuts at: 1050 1050 Size: 4013 SapI GCTCTTCn'nnn_ Cuts at: 2036 2246 2036 Size: 210 3803 SbfI CC_TGCA'GG Cuts at: 1060 1060 Size: 4013 ScaI AGT'ACT Cuts at: 3530 3530 Size: 4013 SfcI C'TryA_G Cuts at: 33 1056 1741 2734 2925 33 Size: 1023 685 993 191 1121 Fragments arranged by size: 1121 1023 993 685 191 SfoI GGC'GCC Cuts at: 1445 1693 1445 Size: 248 3765 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4013 SmaI CCC'GGG Cuts at: 262 1035 262 Size: 773 3240 SmlI C'TyrA_G Cuts at: 1168 2083 2575 2837 1168 Size: 915 492 262 2344 Fragments arranged by size: 2344 915 492 262 SpeI A'CTAG_T Cuts at: 67 807 67 Size: 740 3273 SphI G_CATG'C Cuts at: 1066 1566 1066 Size: 500 3513 StyI C'CwwG_G Cuts at: 107 192 317 1147 1501 107 Size: 85 125 830 354 2619 Fragments arranged by size: 2619 830 354 125 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1596 1729 2188 2397 1596 Size: 133 459 209 3212 Fragments arranged by size: 3212 459 209 133 TatI w'GTAC_w Cuts at: 3528 3528 Size: 4013 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3530 3562 3530 Size: 32 3981 Tth111I GACn'n_nGTC Cuts at: 1810 1810 Size: 4013 XbaI T'CTAG_A Cuts at: 214 1044 214 Size: 830 3183 XcmI CCAnnnn_n'nnnnTGG Cuts at: 324 483 1508 324 Size: 159 1025 2829 Fragments arranged by size: 2829 1025 159 XmaI C'CCGG_G Cuts at: 260 1033 260 Size: 773 3240 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI