Restriction map for pF1KB8783
 (Linear) (Six-base) MAPSORT of: KIBB8783.seq  Check: 8737  from: 1  to: 831



KIBB8783.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     393     831 
   Size:        393     438
 
AccI GT'mk_AC

Cuts at:      0     278     831 
   Size:        278     553
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     831 
   Size:        214     617
 
AflII C'TTAA_G

Cuts at:      0     651     831 
   Size:        651     180
 
AleI CACnn'nnGTG

Cuts at:      0     598     613     831 
   Size:        598      15     218

  Fragments arranged by size: 

                 598     218      15
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     125     631     831 
   Size:        125     506     200

  Fragments arranged by size: 

                 506     200     125
 
AlwNI CAG_nnn'CTG

Cuts at:      0     758     831 
   Size:        758      73
 
ApaLI G'TGCA_C

Cuts at:      0     257     831 
   Size:        257     574
 
ApoI r'AATT_y

Cuts at:      0     824     831 
   Size:        824       7
 
AvaI C'yCGr_G

Cuts at:      0       9     136     547     831 
   Size:          9     127     411     284

  Fragments arranged by size: 

                 411     284     127       9
 
BanI G'GyrC_C

Cuts at:      0     405     831 
   Size:        405     426
 
BanII G_rGCy'C

Cuts at:      0     793     831 
   Size:        793      38
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     198     831 
   Size:        198     633
 
Bme1580I G_kGCm'C

Cuts at:      0     261     410     831 
   Size:        261     149     421

  Fragments arranged by size: 

                 421     261     149
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     638     831 
   Size:        638     193
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     831 
   Size:        112     719
 
Bpu10I CC'TnA_GC

Cuts at:      0     683     831 
   Size:        683     148
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     649     831 
   Size:         28     621     182

  Fragments arranged by size: 

                 621     182      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     591     789     831 
   Size:        131     460     198      42

  Fragments arranged by size: 

                 460     198     131      42
 
BsiHKAI G_wGCw'C

Cuts at:      0     261     831 
   Size:        261     570
 
BsmI GAATG_Cn'

Cuts at:      0     391     699     831 
   Size:        391     308     132

  Fragments arranged by size: 

                 391     308     132
 
Bsp1286I G_dGCh'C

Cuts at:      0     261     410     793     831 
   Size:        261     149     383      38

  Fragments arranged by size: 

                 383     261     149      38
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     393     831 
   Size:        393     438
 
BsrBI CCG'CTC

Cuts at:      0     242     639     831 
   Size:        242     397     192

  Fragments arranged by size: 

                 397     242     192
 
BsrDI GCAATG_nn'

Cuts at:      0     284     831 
   Size:        284     547
 
BssSI C'ACGA_G

Cuts at:      0     251     831 
   Size:        251     580
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     758     831 
   Size:        758      73
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     171     831 
   Size:        171     660
 
BstYI r'GATC_y

Cuts at:      0      86     831 
   Size:         86     745
 
BtgI C'CryG_G

Cuts at:      0     677     831 
   Size:        677     154
 
ClaI AT'CG_AT

Cuts at:      0     318     831 
   Size:        318     513
 
EaeI y'GGCC_r

Cuts at:      0     177     509     831 
   Size:        177     332     322

  Fragments arranged by size: 

                 332     322     177
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     214     831 
   Size:        112     102     617

  Fragments arranged by size: 

                 617     112     102
 
EcoO109I rG'GnC_Cy

Cuts at:      0     687     831 
   Size:        687     144
 
HaeII r_GCGC'y

Cuts at:      0       8     441     831 
   Size:          8     433     390

  Fragments arranged by size: 

                 433     390       8
 
MscI TGG'CCA

Cuts at:      0     511     831 
   Size:        511     320
 
MslI CAynn'nnrTG

Cuts at:      0     598     613     831 
   Size:        598      15     218

  Fragments arranged by size: 

                 598     218      15
 
MspA1I CmG'CkG

Cuts at:      0     115     183     296     818     831 
   Size:        115      68     113     522      13

  Fragments arranged by size: 

                 522     115     113      68      13
 
NcoI C'CATG_G

Cuts at:      0     677     831 
   Size:        677     154
 
NspI r_CATG'y

Cuts at:      0     346     831 
   Size:        346     485
 
PasI CC'CwG_GG

Cuts at:      0     410     831 
   Size:        410     421
 
PfoI T'CCnGG_A

Cuts at:      0      89     831 
   Size:         89     742
 
PstI C_TGCA'G

Cuts at:      0     104     831 
   Size:        104     727
 
PvuII CAG'CTG

Cuts at:      0     115     818     831 
   Size:        115     703      13

  Fragments arranged by size: 

                 703     115      13
 
SbfI CC_TGCA'GG

Cuts at:      0     104     831 
   Size:        104     727
 
SfcI C'TryA_G

Cuts at:      0     100     831 
   Size:        100     731
 
SmaI CCC'GGG

Cuts at:      0      11     831 
   Size:         11     820
 
SmlI C'TyrA_G

Cuts at:      0      43     651     664     831 
   Size:         43     608      13     167

  Fragments arranged by size: 

                 608     167      43      13
 
StuI AGG'CCT

Cuts at:      0      28     427     713     831 
   Size:         28     399     286     118

  Fragments arranged by size: 

                 399     286     118      28
 
StyI C'CwwG_G

Cuts at:      0     677     831 
   Size:        677     154
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     433     810     831 
   Size:        433     377      21

  Fragments arranged by size: 

                 433     377      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     652     684     831 
   Size:        652      32     147

  Fragments arranged by size: 

                 652     147      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     168     831 
   Size:        168     663
 
XmaI C'CCGG_G

Cuts at:      0       9     831 
   Size:          9     822
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII     AleI     AlfI    AlwNI    ApaLI
     ApoI     AvaI     BanI    BanII    BciVI Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
    BsrDI    BssSI   BstAPI    BstXI    BstYI     BtgI     ClaI     EaeI
  Eco57MI EcoO109I    HaeII     MscI     MslI   MspA1I     NcoI     NspI
     PasI     PfoI     PstI    PvuII     SbfI     SfcI     SmaI     SmlI
     StuI     StyI    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrFI    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI
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