Restriction map for pF1KB8776
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BpuAI       BsaAI       BsaBI       
BsaHI       BseAI       BseRI       BsiEI       BsiWI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CfoI        ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MunI        MvnI        MwoI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AgeI         1012  ACCGGT               A^CCGGT
AlwI         1173  GATCC                GGATC(4/5)
AlwNI        1398  CAGCTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII        1525  GGTCC                G^G[AT]CC
BanI         1331  GGCACC               G^G[CT][GA]CC
BbvI         1283  GCTGC                GCAGC(8/12)
BcnI         1056  CCGGG                CC^[GC]GG
BfaI          868  CTAG                 C^TAG
BlpI          812  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      812  GCTCAGC              GC^T[ACGT]AGC
BsaI         1438  GAGACC               GGTCTC(1/5)
BsgI         1118  GTGCAG               GTGCAG(16/14)
BsmBI         242  CGTCTC               CGTCTC(1/5)
BstYI        1172  AGATCC               [GA]^GATC[CT]
CelII         812  GCTCAGC              GC^T[ACGT]AGC
Cfr13I       1525  GGTCC                G^G[ACGT]CC
DpnII        1173  GATC                 ^GATC
DraII        1524  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EarI          645  CTCTTC               CTCTTC(1/4)
EcoO109I     1524  GGGTCCC              [GA]G^G[ACGT]CC[CT]
Fnu4HI       1283  GCTGC                GC^[ACGT]GC
HaeIII       1321  GGCC                 GG^CC
HindIII       797  AAGCTT               A^AGCTT
ItaI         1283  GCTGC                GC^[ACGT]GC
Ksp632I       645  CTCTTC               CTCTTC(1/4)
MaeI          868  CTAG                 C^TAG
MboI         1173  GATC                 ^GATC
MflI         1172  AGATCC               [GA]^GATC[CT]
NciI         1056  CCGGG                CC^[GC]GG
NdeII        1173  GATC                 ^GATC
PinAI        1012  ACCGGT               A^CCGGT
PpuMI        1524  GGGTCCC              [GA]G^G[AT]CC[CT]
Sau3AI       1173  GATC                 ^GATC
Sau96I       1525  GGTCC                G^G[ACGT]CC
SgrAI        1011  CACCGGTG             C[GA]^CCGG[CT]G
SmlI          138  CTTGAG               C^T[CT][GA]AG
TseI         1283  GCTGC                G^C[AT]GC
XhoII        1172  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI          314  CCGC                 CCGC(-3/-1)
             1127  GCGG                
BanII         558  GAGCTC               G[GA]GC[CT]^C
             1566  GAGCTC              
BsaWI         676  ACCGGA               [AT]^CCGG[AT]
             1012  ACCGGT              
BsmI          778  GAATGC               GAATGC(1/-1)
              862  GAATGC              
BsrFI        1012  ACCGGT               [GA]^CCGG[CT]
             1532  GCCGGT              
BstF5I        899  CATCC                GGATG(2/0)
             1571  CATCC               
Cac8I         223  GCAAGC               GC[ACGT]^[ACGT]GC
             1318  GCTGGC              
Cfr10I       1012  ACCGGT               [GA]^CCGG[CT]
             1532  GCCGGT              
DsaI          395  CCATGG               C^C[GA][CT]GG
             1613  CCATGG              
EcoRI         836  GAATTC               G^AATTC
             1424  GAATTC              
EcoT14I       395  CCATGG               C^C[AT][AT]GG
             1613  CCATGG              
FauI          313  CCCGC                CCCGC(4/6)
             1127  GCGGG               
FokI          899  CATCC                GGATG(9/13)
             1571  CATCC               
MboII         413  GAAGA                GAAGA(8/7)
              646  TCTTC               
NcoI          395  CCATGG               C^CATGG
             1613  CCATGG              
PflMI         390  CCATTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1456  CCAGAATGTGG         
SacI          558  GAGCTC               GAGCT^C
             1566  GAGCTC              
StyI          395  CCATGG               C^C[AT][AT]GG
             1613  CCATGG              
TaqI         1226  TCGA                 T^CGA
             1581  TCGA                
Tsp45I        308  GTGAC                ^GT[GC]AC
              359  GTGAC               
TthHB8I      1226  TCGA                 T^CGA
             1581  TCGA                
Van91I        390  CCATTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1456  CCAGAATGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        Asp700I     
AspHI       BmyI        BpmI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstNI       DdeI        EcoRII      
HapII       HinfI       HpaII       HphI        MaeIII      
MnlI        MseI        MslI        MspI        MvaI        
NlaIII      NlaIV       PleI        RsaI        ScrFI       
SfaNI       TfiI        Tru9I       Tsp509I     TspRI       
XmnI        
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