Restriction map for
pF1KB8773
(Circular) (Six-base) MAPSORT of: pF1KB8773.seq Check: 8645 from: 1 to: 3836
pF1KB8773 3836 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 536 536
Size: 3836
AccI GT'mk_AC
Cuts at: 874 874
Size: 3836
Acc65I G'GTAC_C
Cuts at: 852 852
Size: 3836
AclI AA'CG_TT
Cuts at: 3676 3676
Size: 3836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 1661 2093 2840 3108 145
Size: 1516 432 747 268 873
Fragments arranged by size:
1516 873 747 432 268
AflIII A'CryG_T
Cuts at: 224 1188 2292 3269 224
Size: 964 1104 977 791
Fragments arranged by size:
1104 977 964 791
AgeI A'CCGG_T
Cuts at: 2224 2224
Size: 3836
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 631 663 631
Size: 32 3804
AlwNI CAG_nnn'CTG
Cuts at: 487 542 1141 2708 487
Size: 55 599 1567 1615
Fragments arranged by size:
1615 1567 599 55
ApaI G_GGCC'C
Cuts at: 724 724
Size: 3836
ApaLI G'TGCA_C
Cuts at: 2606 2606
Size: 3836
ApoI r'AATT_y
Cuts at: 840 3189 3445 840
Size: 2349 256 1231
Fragments arranged by size:
2349 1231 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3836
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
AvaI C'yCGr_G
Cuts at: 560 736 856 560
Size: 176 120 3540
Fragments arranged by size:
3540 176 120
BamHI G'GATC_C
Cuts at: 861 861
Size: 3836
BanI G'GyrC_C
Cuts at: 354 375 516 524 709 852 1266 1514 1549
Size: 21 141 8 185 143 414 248 35
Cuts at: 1549 354
Size: 2641
Fragments arranged by size:
2641 414 248 185 143 141 35 21
8
BanII G_rGCy'C
Cuts at: 168 269 724 737 850 1880 168
Size: 101 455 13 113 1030 2124
Fragments arranged by size:
2124 1030 455 113 101 13
BbeI G_GCGC'C
Cuts at: 379 1270 1518 379
Size: 891 248 2697
Fragments arranged by size:
2697 891 248
BbsI GAAGACnn'nnnn_
Cuts at: 158 187 158
Size: 29 3807
BbvCI CC'TCA_GC
Cuts at: 507 507
Size: 3836
BciVI GTATCCnnnnn_n'
Cuts at: 1734 2495 3836 1734
Size: 761 1341 1734
Fragments arranged by size:
1734 1341 761
BclI T'GATC_A
Cuts at: 594 1356 594
Size: 762 3074
BglII A'GATC_T
Cuts at: 1351 3388 1351
Size: 2037 1799
BlpI GC'TnA_GC
Cuts at: 948 948
Size: 3836
Bme1580I G_kGCm'C
Cuts at: 441 724 1461 1554 2610 441
Size: 283 737 93 1056 1667
Fragments arranged by size:
1667 1056 737 283 93
BmgBI CAC'GTC
Cuts at: 156 341 657 156
Size: 185 316 3335
Fragments arranged by size:
3335 316 185
BmrI ACTGGGnnnn_n'
Cuts at: 493 1048 1129 1464 3608 493
Size: 555 81 335 2144 721
Fragments arranged by size:
2144 721 555 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 666 2203 666
Size: 1537 2299
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 756 634
Size: 122 3714
Bpu10I CC'TnA_GC
Cuts at: 507 507
Size: 3836
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1891 2383 2681 1012
Size: 879 492 298 2167
Fragments arranged by size:
2167 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 399 820 3540 399
Size: 421 2720 695
Fragments arranged by size:
2720 695 421
BsaAI yAC'GTr
Cuts at: 807 1189 807
Size: 382 3454
BsaBI GATnn'nnATC
Cuts at: 6 1350 3387 6
Size: 1344 2037 455
Fragments arranged by size:
2037 1344 455
BsaHI Gr'CG_yC
Cuts at: 376 1267 1515 2217 3713 376
Size: 891 248 702 1496 499
Fragments arranged by size:
1496 891 702 499 248
BsaWI w'CCGG_w
Cuts at: 1025 1546 2224 2498 2645 3056 1025
Size: 521 678 274 147 411 1805
Fragments arranged by size:
1805 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 394 424 394
Size: 30 3806
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 334 334
Size: 3836
BseYI C'CCAG_C
Cuts at: 628 1261 2019 2596 3092 628
Size: 633 758 577 496 1372
Fragments arranged by size:
1372 758 633 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 437
Size: 3836
BsiEI CG_ry'CG
Cuts at: 104 387 1378 1424 2632 104
Size: 283 991 46 1208 1308
Fragments arranged by size:
1308 1208 991 283 46
BsiHKAI G_wGCw'C
Cuts at: 850 1628 1818 2610 850
Size: 778 190 792 2076
Fragments arranged by size:
2076 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 168 269 441 724 737 850 1461 1554 1628
Size: 101 172 283 13 113 611 93 74
Cuts at: 1628 1818 1880 2610 168
Size: 190 62 730 1394
Fragments arranged by size:
1394 730 611 283 190 172 113 101
93 74 62 13
BspEI T'CCGG_A
Cuts at: 3056 3056
Size: 3836
BspHI T'CATG_A
Cuts at: 3012 3012
Size: 3836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 536 886 1100 1402 1783 536
Size: 350 214 302 381 2589
Fragments arranged by size:
2589 381 350 302 214
BsrBI CCG'CTC
Cuts at: 50 281 372 381 2128 2182 3666 3831 50
Size: 231 91 9 1747 54 1484 165 55
Fragments arranged by size:
1747 1484 231 165 91 55 54 9
BsrDI GCAATG_nn'
Cuts at: 290 1748 290
Size: 1458 2378
BsrFI r'CCGG_y
Cuts at: 172 1834 2015 2224 172
Size: 1662 181 209 1784
Fragments arranged by size:
1784 1662 209 181
BssSI C'ACGA_G
Cuts at: 116 145 2107 2465 116
Size: 29 1962 358 1487
Fragments arranged by size:
1962 1487 358 29
BstAPI GCAn_nnn'nTGC
Cuts at: 445 1213 445
Size: 768 3068
BstBI TT'CG_AA
Cuts at: 2197 2197
Size: 3836
BstXI CCAn_nnnn'nTGG
Cuts at: 636 636
Size: 3836
BstYI r'GATC_y
Cuts at: 1 311 404 815 861 1328 1351 1686 1932
Size: 310 93 411 46 467 23 335 246
Cuts at: 1932 2944 3030 3042 3388 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 411 346 335 310 246
93 86 46 23 12
BtgI C'CryG_G
Cuts at: 398 398
Size: 3836
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1786 1967 2130 1786
Size: 181 163 3492
Fragments arranged by size:
3492 181 163
BtsI GCAGTG_nn'
Cuts at: 1079 1079
Size: 3836
DraI TTT'AAA
Cuts at: 835 835
Size: 3836
DraIII CAC_nnn'GTG
Cuts at: 810 810
Size: 3836
DrdI GACnn_nn'nnGTC
Cuts at: 391 1542 2400 391
Size: 1151 858 1827
Fragments arranged by size:
1827 1151 858
EaeI y'GGCC_r
Cuts at: 141 170 465 752 1421 1595 1986 2013 3358
Size: 29 295 287 669 174 391 27 1345
Cuts at: 3358 141
Size: 619
Fragments arranged by size:
1345 669 619 391 295 287 174 29
27
EagI C'GGCC_G
Cuts at: 1421 1421
Size: 3836
EarI CTCTTCn'nnn_
Cuts at: 121 635 1859 2069 121
Size: 514 1224 210 1888
Fragments arranged by size:
1888 1224 514 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2044 2354 2500 3646 2044
Size: 310 146 1146 2234
Fragments arranged by size:
2234 1146 310 146
EcoICRI GAG'CTC
Cuts at: 848 848
Size: 3836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 634 756 1661 2093 2840 3108 145
Size: 489 122 905 432 747 268 873
Fragments arranged by size:
905 873 747 489 432 268 122
EcoO109I rG'GnC_Cy
Cuts at: 721 763 975 721
Size: 42 212 3582
Fragments arranged by size:
3582 212 42
EcoRI G'AATT_C
Cuts at: 840 840
Size: 3836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1855 1855
Size: 3836
FspI TGC'GCA
Cuts at: 1617 1617
Size: 3836
HaeII r_GCGC'y
Cuts at: 379 542 1270 1518 2540 3519 379
Size: 163 728 248 1022 979 696
Fragments arranged by size:
1022 979 728 696 248 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1678 1708 1710 1740 1678
Size: 30 2 30 3774
Fragments arranged by size:
3774 30 30 2
HincII GTy'rAC
Cuts at: 875 875
Size: 3836
HindIII A'AGCT_T
Cuts at: 576 576
Size: 3836
KasI G'GCGC_C
Cuts at: 375 1266 1514 375
Size: 891 248 2697
Fragments arranged by size:
2697 891 248
KpnI G_GTAC'C
Cuts at: 856 856
Size: 3836
MluI A'CGCG_T
Cuts at: 3269 3269
Size: 3836
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 809 2507 2691 809
Size: 1698 184 1954
Fragments arranged by size:
1954 1698 184
MscI TGG'CCA
Cuts at: 143 1597 143
Size: 1454 2382
MslI CAynn'nnrTG
Cuts at: 259 1825 1952 259
Size: 1566 127 2143
Fragments arranged by size:
2143 1566 127
MspA1I CmG'CkG
Cuts at: 207 632 776 947 1261 1621 2634 2879 207
Size: 425 144 171 314 360 1013 245 1164
Fragments arranged by size:
1164 1013 425 360 314 245 171 144
NaeI GCC'GGC
Cuts at: 174 2017 174
Size: 1843 1993
NarI GG'CG_CC
Cuts at: 376 1267 1515 376
Size: 891 248 2697
Fragments arranged by size:
2697 891 248
NgoMIV G'CCGG_C
Cuts at: 172 2015 172
Size: 1843 1993
NspI r_CATG'y
Cuts at: 228 258 889 1389 2296 228
Size: 30 631 500 907 1768
Fragments arranged by size:
1768 907 631 500 30
PciI A'CATG_T
Cuts at: 224 2292 224
Size: 2068 1768
PflMI CCAn_nnn'nTGG
Cuts at: 810 810
Size: 3836
PfoI T'CCnGG_A
Cuts at: 218 218
Size: 3836
PmeI GTTT'AAAC
Cuts at: 835 835
Size: 3836
PmlI CAC'GTG
Cuts at: 807 807
Size: 3836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2975 3007 2975
Size: 32 3804
PspOMI G'GGCC_C
Cuts at: 720 720
Size: 3836
PspXI vC'TCGA_Gb
Cuts at: 736 736
Size: 3836
PstI C_TGCA'G
Cuts at: 206 369 533 883 1568 206
Size: 163 164 350 685 2474
Fragments arranged by size:
2474 685 350 164 163
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3836
PvuII CAG'CTG
Cuts at: 632 776 1261 1621 632
Size: 144 485 360 2847
Fragments arranged by size:
2847 485 360 144
RsrII CG'GwC_CG
Cuts at: 303 2031 303
Size: 1728 2108
SacI G_AGCT'C
Cuts at: 850 850
Size: 3836
SalI G'TCGA_C
Cuts at: 873 873
Size: 3836
SapI GCTCTTCn'nnn_
Cuts at: 635 1859 2069 635
Size: 1224 210 2402
Fragments arranged by size:
2402 1224 210
SbfI CC_TGCA'GG
Cuts at: 883 883
Size: 3836
ScaI AGT'ACT
Cuts at: 689 3353 689
Size: 2664 1172
SfcI C'TryA_G
Cuts at: 33 202 365 529 879 1564 2557 2748 33
Size: 169 163 164 350 685 993 191 1121
Fragments arranged by size:
1121 993 685 350 191 169 164 163
SfoI GGC'GCC
Cuts at: 377 1268 1516 377
Size: 891 248 2697
Fragments arranged by size:
2697 891 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3836
SmaI CCC'GGG
Cuts at: 858 858
Size: 3836
SmlI C'TyrA_G
Cuts at: 560 736 991 1906 2398 2660 560
Size: 176 255 915 492 262 1736
Fragments arranged by size:
1736 915 492 262 255 176
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3836
SphI G_CATG'C
Cuts at: 889 1389 889
Size: 500 3336
StyI C'CwwG_G
Cuts at: 520 970 1324 520
Size: 450 354 3032
Fragments arranged by size:
3032 450 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1419 1552 2011 2220 1419
Size: 133 459 209 3035
Fragments arranged by size:
3035 459 209 133
TatI w'GTAC_w
Cuts at: 687 3351 687
Size: 2664 1172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 325 357 394 426 462 494 3353 3385 325
Size: 32 37 32 36 32 2859 32 776
Fragments arranged by size:
2859 776 37 36 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 186 1633 186
Size: 1447 2389
XbaI T'CTAG_A
Cuts at: 867 867
Size: 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1331 1331
Size: 3836
XhoI C'TCGA_G
Cuts at: 560 736 560
Size: 176 3660
XmaI C'CCGG_G
Cuts at: 856 856
Size: 3836
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BcgI BfrBI BglI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PpuMI PshAI PsiI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XmnI ZraI