Restriction map for pF1KB8760
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AgeI        
AhdI        AlwI        AocI        ApaI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      EaeI        EagI        
Eam1105I    EarI        EclXI       Eco52I      Eco81I      
EcoRI       EcoT22I     FbaI        FseI        FspI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     MaeI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccII         163  CGCG                 CG^CG
AflIII        566  ACATGT               A^C[GA][CT]GT
Alw44I        507  GTGCAC               G^TGCAC
AlwNI         418  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       584  AGCGCT               AGC^GCT
ApaLI         507  GTGCAC               G^TGCAC
AspHI         507  GTGCAC               G[AT]GC[AT]^C
AspI            6  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          367  CCCGGG               C^[CT]CG[GA]G
BanII         434  GGGCTC               G[GA]GC[CT]^C
BglII          38  AGATCT               A^GATCT
BsiHKAI       507  GTGCAC               G[AT]GC[AT]^C
BsmBI           4  GAGACG               CGTCTC(1/5)
BsmFI          12  GTCCC                GGGAC(10/14)
BsoBI         367  CCCGGG               C^[CT]CG[GA]G
BspLU11I      566  ACATGT               A^CATGT
BspMI          97  ACCTGC               ACCTGC(4/8)
BsrDI         287  GCAATG               GCAATG(2/0)
BstEII        396  GGTGACC              G^GT[ACGT]ACC
BstPI         396  GGTGACC              G^GT[ACGT]ACC
BstUI         163  CGCG                 CG^CG
BstXI          74  CCAAGTTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          38  AGATCT               [GA]^GATC[CT]
DdeI          581  CTAAG                C^T[ACGT]AG
DpnII          39  GATC                 ^GATC
DraII         221  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI          214  GACCCGGAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      584  AGCGCT               AGC^GCT
EcoNI         421  CCTCTGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      221  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       396  GGTGACC              G^GT[ACGT]ACC
EcoRV         597  GATATC               GAT^ATC
EcoT14I       320  CCAAGG               C^C[AT][AT]GG
FauI          471  GCGGG                CCCGC(4/6)
HaeII         584  AGCGCT               [GA]GCGC^[CT]
HaeIII         71  GGCC                 GG^CC
HinfI          19  GACTC                G^A[ACGT]TC
KspI          162  CCGCGG               CCGC^GG
MboI           39  GATC                 ^GATC
MflI           38  AGATCT               [GA]^GATC[CT]
MspA1I        162  CCGCGG               C[AC]G^C[GT]G
MvnI          163  CGCG                 CG^CG
NdeII          39  GATC                 ^GATC
PflFI           6  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI           19  GACTC                GAGTC(4/5)
PpuMI         221  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          115  CTGCAG               CTGCA^G
SacII         162  CCGCGG               CCGC^GG
Sau3AI         39  GATC                 ^GATC
SexAI         493  ACCTGGT              A^CC[AT]GGT
SfaNI         551  GCATC                GCATC(5/9)
SmaI          367  CCCGGG               CCC^GGG
StyI          320  CCAAGG               C^C[AT][AT]GG
Tsp509I        49  AATT                 ^AATT
Tth111I         6  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII          38  AGATCT               [GA]^GATC[CT]
XmaCI         367  CCCGGG               C^CCGGG
XmaI          367  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AvaII         128  GGACC                G^G[AT]CC
              222  GGTCC               
BanI          149  GGTGCC               G^G[CT][GA]CC
              158  GGTGCC              
BbsI          268  GTCTTC               GAAGAC(2/6)
              538  GTCTTC              
BpuAI         268  GTCTTC               GAAGAC(2/6)
              538  GTCTTC              
BsmAI           4  GAGAC                GTCTC(1/5)
              344  GAGAC               
BstAPI        287  GCAATGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              417  GCAGCCTCTGC         
BstF5I        402  CATCC                GGATG(2/0)
              141  GGATG               
DsaI          162  CCGCGG               C^C[GA][CT]GG
              392  CCGTGG              
FokI          402  CATCC                GGATG(9/13)
              141  GGATG               
HgaI            6  GACGC                GACGC(5/10)
              530  GCGTC               
MboII         269  TCTTC                GAAGA(8/7)
              539  TCTTC               
MslI          390  CACCGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              516  CATCTTGGTG          
NspI          299  ACATGC               [GA]CATG^[CT]
              566  ACATGT              
SfcI           54  CTACAG               C^T[GA][CT]AG
              115  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AluI        BbvI        BcnI        BmyI        
BsaJI       BseRI       BsiYI       BslI        Bsp1286I    
BssKI       BstNI       Cac8I       CfoI        Cfr13I      
EcoRII      Fnu4HI      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MaeIII      MnlI        
MspI        MvaI        MwoI        NciI        NlaIII      
NlaIV       Sau96I      ScrFI       TseI        Tsp45I      
TspRI       
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