Restriction map for pF1KB8760
 (Linear) (Six-base) MAPSORT of: KIBB8760.seq  Check: 9763  from: 1  to: 609



KIBB8760.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     106     609 
   Size:        106     503
 
AfeI AGC'GCT

Cuts at:      0     586     609 
   Size:        586      23
 
AflIII A'CryG_T

Cuts at:      0     566     609 
   Size:        566      43
 
AleI CACnn'nnGTG

Cuts at:      0     394     609 
   Size:        394     215
 
AlwNI CAG_nnn'CTG

Cuts at:      0     423     609 
   Size:        423     186
 
ApaLI G'TGCA_C

Cuts at:      0     507     609 
   Size:        507     102
 
AvaI C'yCGr_G

Cuts at:      0     367     609 
   Size:        367     242
 
BanI G'GyrC_C

Cuts at:      0     149     158     609 
   Size:        149       9     451

  Fragments arranged by size: 

                 451     149       9
 
BanII G_rGCy'C

Cuts at:      0     438     609 
   Size:        438     171
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     261     531     609 
   Size:        261     270      78

  Fragments arranged by size: 

                 270     261      78
 
BglII A'GATC_T

Cuts at:      0      38     609 
   Size:         38     571
 
Bme1580I G_kGCm'C

Cuts at:      0     154     511     609 
   Size:        154     357      98

  Fragments arranged by size: 

                 357     154      98
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25     609 
   Size:         25     584
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     469     472     535     609 
   Size:        469       3      63      74

  Fragments arranged by size: 

                 469      74      63       3
 
BseYI C'CCAG_C

Cuts at:      0     283     609 
   Size:        283     326
 
BsiHKAI G_wGCw'C

Cuts at:      0     511     609 
   Size:        511      98
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     438     511     609 
   Size:        154     284      73      98

  Fragments arranged by size: 

                 284     154      98      73
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     106     609 
   Size:        106     503
 
BsrDI GCAATG_nn'

Cuts at:      0     294     609 
   Size:        294     315
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     293     423     609 
   Size:        293     130     186

  Fragments arranged by size: 

                 293     186     130
 
BstEII G'GTnAC_C

Cuts at:      0     396     609 
   Size:        396     213
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      81     609 
   Size:         81     528
 
BstYI r'GATC_y

Cuts at:      0      38     609 
   Size:         38     571
 
BtgI C'CryG_G

Cuts at:      0     162     392     609 
   Size:        162     230     217

  Fragments arranged by size: 

                 230     217     162
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     219     609 
   Size:        219     390
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     220     609 
   Size:        220     389
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     425     609 
   Size:        425     184
 
EcoO109I rG'GnC_Cy

Cuts at:      0     222     609 
   Size:        222     387
 
EcoRV GAT'ATC

Cuts at:      0     599     609 
   Size:        599      10
 
HaeII r_GCGC'y

Cuts at:      0     588     609 
   Size:        588      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     609 
   Size:         27     582
 
MslI CAynn'nnrTG

Cuts at:      0     394     520     609 
   Size:        394     126      89

  Fragments arranged by size: 

                 394     126      89
 
MspA1I CmG'CkG

Cuts at:      0     164     609 
   Size:        164     445
 
NspI r_CATG'y

Cuts at:      0     303     570     609 
   Size:        303     267      39

  Fragments arranged by size: 

                 303     267      39
 
PciI A'CATG_T

Cuts at:      0     566     609 
   Size:        566      43
 
PfoI T'CCnGG_A

Cuts at:      0     206     609 
   Size:        206     403
 
PpuMI rG'GwC_Cy

Cuts at:      0     222     609 
   Size:        222     387
 
PstI C_TGCA'G

Cuts at:      0     119     609 
   Size:        119     490
 
SacII CC_GC'GG

Cuts at:      0     165     609 
   Size:        165     444
 
SexAI A'CCwGG_T

Cuts at:      0     493     609 
   Size:        493     116
 
SfcI C'TryA_G

Cuts at:      0      54     115     609 
   Size:         54      61     494

  Fragments arranged by size: 

                 494      61      54
 
SmaI CCC'GGG

Cuts at:      0     369     609 
   Size:        369     240
 
StyI C'CwwG_G

Cuts at:      0     320     609 
   Size:        320     289
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     281     609 
   Size:        281     328
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     133     165     609 
   Size:        133      32     444

  Fragments arranged by size: 

                 444     133      32
 
Tth111I GACn'n_nGTC

Cuts at:      0       9     609 
   Size:          9     600
 
XmaI C'CCGG_G

Cuts at:      0     367     609 
   Size:        367     242
 

 Enzymes that do cut:

     AarI     AfeI   AflIII     AleI    AlwNI    ApaLI     AvaI     BanI
    BanII     BbsI    BglII Bme1580I    BsaXI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspMI    BsrDI   BstAPI   BstEII    BstXI    BstYI     BtgI
    BtgZI     DrdI    EcoNI EcoO109I    EcoRV    HaeII    Hin4I     MslI
   MspA1I     NspI     PciI     PfoI    PpuMI     PstI    SacII    SexAI
     SfcI     SmaI     StyI    TaqII     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     XbaI     XcmI     XhoI     XmnI     ZraI
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