Restriction map for pF1KB8754
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AlwI        AocI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DdeI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        Hin1I       
HincII      HindII      HindIII     HinfI       HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp509I     TspRI       Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

Alw44I        162  GTGCAC               G^TGCAC
Aor51HI       539  AGCGCT               AGC^GCT
ApaI          292  GGGCCC               GGGCC^C
ApaLI         162  GTGCAC               G^TGCAC
AspI          565  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BclI          140  TGATCA               T^GATCA
BsaI          442  GAGACC               GGTCTC(1/5)
BseRI         283  GAGGAG               GAGGAG(10/8)
BsgI          313  GTGCAG               GTGCAG(16/14)
BspMI         135  GCAGGT               ACCTGC(4/8)
BsrI          198  CCAGT                ACTGG(1/-1)
BssSI         352  CTCGTG               CACGAG(-5/-1)
BstAPI        359  GCAACAAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII         291  GGGGCCC              [GA]G^G[ACGT]CC[CT]
Eco47III      539  AGCGCT               AGC^GCT
EcoO109I      291  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       230  CCAAGG               C^C[AT][AT]GG
FbaI          140  TGATCA               T^GATCA
PflFI         565  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          169  CTGCAG               CTGCA^G
PvuII         144  CAGCTG               CAG^CTG
SapI          477  GCTCTTC              GCTCTTC(1/4)
SfaNI         348  GATGC                GCATC(5/9)
SfcI          169  CTGCAG               C^T[GA][CT]AG
StyI          230  CCAAGG               C^C[AT][AT]GG
Tsp45I        262  GTCAC                ^GT[GC]AC
Tth111I       565  GACCGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BcnI          204  CCCGG                CC^[GC]GG
              545  CCGGG               
BpmI          280  CTGGAG               CTGGAG(16/14)
              499  CTGGAG              
BsmBI         373  GAGACG               CGTCTC(1/5)
              502  GAGACG              
BsrFI         131  ACCGGC               [GA]^CCGG[CT]
              187  ACCGGC              
Cfr10I        131  ACCGGC               [GA]^CCGG[CT]
              187  ACCGGC              
EaeI          206  CGGCCA               [CT]^GGCC[GA]
              449  CGGCCA              
EarI          478  CTCTTC               CTCTTC(1/4)
              555  GAAGAG              
FauI           43  GCGGG                CCCGC(4/6)
              381  GCGGG               
HaeII         215  AGCGCC               [GA]GCGC^[CT]
              539  AGCGCT              
HphI          138  GGTGA                GGTGA(8/7)
              263  TCACC               
Ksp632I       478  CTCTTC               CTCTTC(1/4)
              555  GAAGAG              
MaeII          91  ACGT                 A^CGT
              467  ACGT                
MaeIII         14  GTAAC                ^GT[ACGT]AC
              262  GTCAC               
MboII         555  GAAGA                GAAGA(8/7)
              479  TCTTC               
MspA1I        144  CAGCTG               C[AC]G^C[GT]G
              379  CAGCGG              
NciI          204  CCCGG                CC^[GC]GG
              545  CCGGG               
NlaIV         291  GGGGCC               GG[ACGT]^[ACGT]CC
              292  GGGCCC              
SacI           60  GAGCTC               GAGCT^C
              475  GAGCTC              
TaqI          119  TCGA                 T^CGA
              530  TCGA                
TthHB8I       119  TCGA                 T^CGA
              530  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AspHI       
BanII       BbvI        BmyI        BsaJI       BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HpaII       ItaI        MboI        MnlI        MslI        
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      Sau3AI      Sau96I      ScrFI       TseI        
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