Restriction map for pF1KB8751
##################################
# NO CUT ENZYMES (  193 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MaeII       MamI        
MfeI        MflI        MluI        MroI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
StyI        SwaI        TaqI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AlwNI         367  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaI          197  CCCGAG               C^[CT]CG[GA]G
AvaII         327  GGACC                G^G[AT]CC
BbvI          216  GCAGC                GCAGC(8/12)
BpmI           46  CTCCAG               CTGGAG(16/14)
BsaWI         480  ACCGGA               [AT]^CCGG[AT]
BseRI         507  CTCCTC               GAGGAG(10/8)
BsoBI         197  CCCGAG               C^[CT]CG[GA]G
BspMI         329  ACCTGC               ACCTGC(4/8)
BssKI          67  CCTGG                ^CC[ACGT]GG
BstNI          67  CCTGG                CC^[AT]GG
BstXI         128  CCAGCGAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI          240  CTCAG                C^T[ACGT]AG
DraI          223  TTTAAA               TTT^AAA
EarI           28  GAAGAG               CTCTTC(1/4)
EcoRII         67  CCTGG                ^CC[AT]GG
Fnu4HI        216  GCAGC                GC^[ACGT]GC
HapII         481  CCGG                 C^CGG
HpaII         481  CCGG                 C^CGG
HphI          138  GGTGA                GGTGA(8/7)
ItaI          216  GCAGC                GC^[ACGT]GC
Ksp632I        28  GAAGAG               CTCTTC(1/4)
MaeIII         37  GTAAC                ^GT[ACGT]AC
MnlI          509  CCTC                 CCTC(7/6)
MslI          335  CATACAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        217  CAGCTG               C[AC]G^C[GT]G
MspI          481  CCGG                 C^CGG
MvaI           67  CCTGG                CC^[AT]GG
NlaIII        345  CATG                 CATG^
PstI          214  CTGCAG               CTGCA^G
PvuII         217  CAGCTG               CAG^CTG
ScrFI          67  CCTGG                CC^[ACGT]GG
SfaNI          16  GATGC                GCATC(5/9)
SfcI          214  CTGCAG               C^T[GA][CT]AG
SspI           87  AATATT               AAT^ATT
TfiI          353  GATTC                G^A[AT]TC
TseI          216  GCAGC                G^C[AT]GC
TspRI          79  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BalI           69  TGGCCA               TGG^CCA
              460  TGGCCA              
BsiYI          48  CCAGATGACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              481  CCGGAGTATGG         
BslI           48  CCAGATGACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              481  CCGGAGTATGG         
BstF5I         64  CATCC                GGATG(2/0)
              537  GGATG               
Cfr13I        327  GGACC                G^G[ACGT]CC
              473  GGCCC               
DpnII         288  GATC                 ^GATC
              437  GATC                
FokI           64  CATCC                GGATG(9/13)
              537  GGATG               
MboI          288  GATC                 ^GATC
              437  GATC                
MluNI          69  TGGCCA               TGG^CCA
              460  TGGCCA              
MscI           69  TGGCCA               TGG^CCA
              460  TGGCCA              
MseI          107  TTAA                 T^TAA
              224  TTAA                
NdeII         288  GATC                 ^GATC
              437  GATC                
Sau3AI        288  GATC                 ^GATC
              437  GATC                
Sau96I        327  GGACC                G^G[ACGT]CC
              473  GGCCC               
Tru9I         107  TTAA                 T^TAA
              224  TTAA                
XcmI           72  CCAGTTGCAGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              281  CCAATCTGATCGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AcsI        AluI        ApoI        BsmAI       BsrI        
EaeI        HaeIII      HinfI       MboII       PleI        
Tsp509I     
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