Restriction map for pF1KB8745
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AfaI        AgeI        AhdI        Alw44I      ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BbeI        BbsI        BcgI        BciVI       BcnI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaI        BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstYI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MflI        MluI        MroI        
MunI        NarI        NciI        NdeI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccII         283  CGCG                 CG^CG
AcyI          208  GGCGTC               G[GA]^CG[CT]C
AflII         574  CTTAAG               C^TTAAG
AflIII         18  ACGTGT               A^C[GA][CT]GT
AlwNI        1132  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       251  AGCGCT               AGC^GCT
Asp700I       135  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          144  CTCGGG               C^[CT]CG[GA]G
BalI          110  TGGCCA               TGG^CCA
BbrPI          17  CACGTG               CAC^GTG
BbvCI          75  GCTGAGG              CCTCAGC(-5/-2)
BclI         1118  TGATCA               T^GATCA
BfaI            5  CTAG                 C^TAG
BfrI          574  CTTAAG               C^TTAAG
BglI          112  GCCAGCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI          17  CACGTG               [CT]AC^GT[GA]
BsaBI         759  GATAGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         208  GGCGTC               G[GA]^CG[CT]C
BsaWI         512  ACCGGA               [AT]^CCGG[AT]
BseRI         235  GAGGAG               GAGGAG(10/8)
BsgI          460  GTGCAG               GTGCAG(16/14)
BsmI           42  GAATGC               GAATGC(1/-1)
BsoBI         144  CTCGGG               C^[CT]CG[GA]G
BsrBI         610  GAGCGG               CCGCTC(-3/-3)
BsrFI          32  GCCGGC               [GA]^CCGG[CT]
BstUI         283  CGCG                 CG^CG
CfoI          252  GCGC                 GCG^C
Cfr10I         32  GCCGGC               [GA]^CCGG[CT]
DsaI          390  CCATGG               C^C[GA][CT]GG
EaeI          110  TGGCCA               [CT]^GGCC[GA]
EarI          655  GAAGAG               CTCTTC(1/4)
Eco47III      251  AGCGCT               AGC^GCT
FauI          281  CCCGC                CCCGC(4/6)
FbaI         1118  TGATCA               T^GATCA
HaeII         251  AGCGCT               [GA]GCGC^[CT]
HhaI          252  GCGC                 GCG^C
Hin1I         208  GGCGTC               G[GA]^CG[CT]C
HinP1I        252  GCGC                 G^CGC
Ksp632I       655  GAAGAG               CTCTTC(1/4)
MaeI            5  CTAG                 C^TAG
MaeII          18  ACGT                 A^CGT
MamI          759  GATAGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         110  TGGCCA               TGG^CCA
MscI          110  TGGCCA               TGG^CCA
MslI          291  CATGCGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1035  CCGCTG               C[AC]G^C[GT]G
MvnI          283  CGCG                 CG^CG
NaeI           32  GCCGGC               GCC^GGC
NcoI          390  CCATGG               C^CATGG
NgoMIV         32  GCCGGC               G^CCGGC
NheI            4  GCTAGC               G^CTAGC
PmaCI          17  CACGTG               CAC^GTG
PmlI           17  CACGTG               CAC^GTG
PshAI         195  GACCCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
TaqI          809  TCGA                 T^CGA
TfiI          925  GATTC                G^A[AT]TC
TthHB8I       809  TCGA                 T^CGA
XcmI          856  CCACTTCCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          135  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI          397  AAATTT               [GA]^AATT[CT]
             1113  GAATTT              
AlwI          447  GATCC                GGATC(4/5)
              831  GATCC               
AocI          267  CCTTAGG              CC^T[ACGT]AGG
              901  CCTCAGG             
ApaI           62  GGGCCC               GGGCC^C
              273  GGGCCC              
ApoI          397  AAATTT               [GA]^AATT[CT]
             1113  GAATTT              
BanI          156  GGTGCC               G^G[CT][GA]CC
              871  GGTGCC              
BsmFI         557  GGGAC                GGGAC(10/14)
               81  GTCCC               
BsrDI          12  CATTGC               GCAATG(2/0)
              782  GCAATG              
BsrI          188  ACTGG                ACTGG(1/-1)
              962  CCAGT               
Bsu36I        267  CCTTAGG              CC^T[ACGT]AGG
              901  CCTCAGG             
Eco81I        267  CCTTAGG              CC^T[ACGT]AGG
              901  CCTCAGG             
HapII          33  CCGG                 C^CGG
              513  CCGG                
HgaI          209  GCGTC                GACGC(5/10)
              690  GCGTC               
HpaII          33  CCGG                 C^CGG
              513  CCGG                
HphI          544  GGTGA                GGTGA(8/7)
             1051  GGTGA               
MboII         172  GAAGA                GAAGA(8/7)
              655  GAAGA               
MspI           33  CCGG                 C^CGG
              513  CCGG                


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BbvI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstF5I      BstNI       
BstXI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HinfI       ItaI        MaeIII      
MboI        MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
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