Restriction map for pF1KB8741
##################################
# NO CUT ENZYMES (  202 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstF5I      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DdeI        
DpnII       DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeII       MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tsp45I      Tth111I     
TthHB8I     Van91I      XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AciI          301  CCGC                 CCGC(-3/-1)
AfaI          409  GTAC                 GT^AC
AlwNI         155  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BanI          283  GGTGCC               G^G[CT][GA]CC
BbsI           81  GAAGAC               GAAGAC(2/6)
BglI          163  GCCTGGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI          81  GAAGAC               GAAGAC(2/6)
BsrI          338  ACTGG                ACTGG(1/-1)
BstAPI        168  GCAGGCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         331  CCAAGCCACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         168  GCAGGC               GC[ACGT]^[ACGT]GC
DraI          253  TTTAAA               TTT^AAA
DraIII        308  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I        86  CCAAGG               C^C[AT][AT]GG
HaeIII        171  GGCC                 GG^CC
HphI          307  TCACC                GGTGA(8/7)
MaeIII        246  GTTAC                ^GT[ACGT]AC
MspA1I        158  CAGCTG               C[AC]G^C[GT]G
PvuII         158  CAGCTG               CAG^CTG
RsaI          409  GTAC                 GT^AC
SmlI          260  CTCAAG               C^T[CT][GA]AG
StyI           86  CCAAGG               C^C[AT][AT]GG
TspRI         337  CACTG                CA[GC]TG[ACGT][ACGT]^
XbaI           40  TCTAGA               T^CTAGA
XcmI          328  CCACCAAGCCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AvaII         238  GGTCC                G^G[AT]CC
              342  GGTCC               
BbvI          157  GCAGC                GCAGC(8/12)
              160  GCTGC               
BfaI           41  CTAG                 C^TAG
              227  CTAG                
BsaJI          86  CCAAGG               C^C[ACGT][ACGT]GG
              241  CCCAGG              
BsmFI         239  GTCCC                GGGAC(10/14)
              343  GTCCC               
BssKI         164  CCTGG                ^CC[ACGT]GG
              242  CCAGG               
BstNI         164  CCTGG                CC^[AT]GG
              242  CCAGG               
Cfr13I        238  GGTCC                G^G[ACGT]CC
              342  GGTCC               
DraII         237  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              341  GGGTCCC             
EcoO109I      237  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              341  GGGTCCC             
EcoRI          96  GAATTC               G^AATTC
              222  GAATTC              
EcoRII        164  CCTGG                ^CC[AT]GG
              242  CCAGG               
MaeI           41  CTAG                 C^TAG
              227  CTAG                
MvaI          164  CCTGG                CC^[AT]GG
              242  CCAGG               
MwoI          163  GCCTGGCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              168  GCAGGCCATGC         
NlaIII        139  CATG                 CATG^
              174  CATG                
PpuMI         237  AGGTCCC              [GA]G^G[AT]CC[CT]
              341  GGGTCCC             
Sau96I        238  GGTCC                G^G[ACGT]CC
              342  GGTCC               
ScrFI         164  CCTGG                CC^[ACGT]GG
              242  CCAGG               
TseI          157  GCAGC                G^C[AT]GC
              160  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AcsI        AluI        ApoI        Fnu4HI      ItaI        
MboII       MnlI        MseI        NlaIV       Tru9I       
Tsp509I     
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