Restriction map for pF1KB8738
 (Circular) (Six-base) MAPSORT of: pF1KB8738.seq  Check: 5439  from: 1  to: 3446



pF1KB8738  3446 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:29  ..

AccI GT'mk_AC

Cuts at:    484     484 
   Size:       3446
 
Acc65I G'GTAC_C

Cuts at:    462     462 
   Size:       3446
 
AclI AA'CG_TT

Cuts at:   3286    3286 
   Size:       3446
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1271    1703    2450    2718    1271 
   Size:        432     747     268    1999

  Fragments arranged by size: 

                1999     747     432     268
 
AflIII A'CryG_T

Cuts at:    798    1902    2879     798 
   Size:       1104     977    1365

  Fragments arranged by size: 

                1365    1104     977
 
AgeI A'CCGG_T

Cuts at:   1834    1834 
   Size:       3446
 
AlwNI CAG_nnn'CTG

Cuts at:    751    2318     751 
   Size:       1567    1879
 
ApaLI G'TGCA_C

Cuts at:   2216    2216 
   Size:       3446
 
ApoI r'AATT_y

Cuts at:    424     450    2799    3055     424 
   Size:         26    2349     256     815

  Fragments arranged by size: 

                2349     815     256      26
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3446
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3446
 
AvaI C'yCGr_G

Cuts at:    466     466 
   Size:       3446
 
BamHI G'GATC_C

Cuts at:    471     471 
   Size:       3446
 
BanI G'GyrC_C

Cuts at:    462     876    1124    1159     462 
   Size:        414     248      35    2749

  Fragments arranged by size: 

                2749     414     248      35
 
BanII G_rGCy'C

Cuts at:    398     460    1490     398 
   Size:         62    1030    2354

  Fragments arranged by size: 

                2354    1030      62
 
BbeI G_GCGC'C

Cuts at:    880    1128     880 
   Size:        248    3198
 
BbsI GAAGACnn'nnnn_

Cuts at:    173     173 
   Size:       3446
 
BciVI GTATCCnnnnn_n'

Cuts at:    391    1344    2105    3446     391 
   Size:        953     761    1341     391

  Fragments arranged by size: 

                1341     953     761     391
 
BclI T'GATC_A

Cuts at:    966     966 
   Size:       3446
 
BglII A'GATC_T

Cuts at:    961    2998     961 
   Size:       2037    1409
 
BlpI GC'TnA_GC

Cuts at:    558     558 
   Size:       3446
 
Bme1580I G_kGCm'C

Cuts at:   1071    1164    2220    1071 
   Size:         93    1056    2297

  Fragments arranged by size: 

                2297    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    658     739    1074    3218     658 
   Size:         81     335    2144     886

  Fragments arranged by size: 

                2144     886     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1813    1813 
   Size:       3446
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    622    1501    1993    2291     622 
   Size:        879     492     298    1777

  Fragments arranged by size: 

                1777     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3150    3150 
   Size:       3446
 
BsaAI yAC'GTr

Cuts at:    799     799 
   Size:       3446
 
BsaBI GATnn'nnATC

Cuts at:      6     960    2997       6 
   Size:        954    2037     455

  Fragments arranged by size: 

                2037     954     455
 
BsaHI Gr'CG_yC

Cuts at:    877    1125    1827    3323     877 
   Size:        248     702    1496    1000

  Fragments arranged by size: 

                1496    1000     702     248
 
BsaWI w'CCGG_w

Cuts at:    635    1156    1834    2108    2255    2666     635 
   Size:        521     678     274     147     411    1415

  Fragments arranged by size: 

                1415     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    346     376     346 
   Size:         30    3416
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    197     337     197 
   Size:        140    3306
 
BseYI C'CCAG_C

Cuts at:    871    1629    2206    2702     871 
   Size:        758     577     496    1615

  Fragments arranged by size: 

                1615     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     988    1034    2242     104 
   Size:        884      46    1208    1308

  Fragments arranged by size: 

                1308    1208     884      46
 
BsiHKAI G_wGCw'C

Cuts at:    398     460    1238    1428    2220     398 
   Size:         62     778     190     792    1624

  Fragments arranged by size: 

                1624     792     778     190      62
 
Bsp1286I G_dGCh'C

Cuts at:    398     460    1071    1164    1238    1428    1490    2220     398 
   Size:         62     611      93      74     190      62     730    1624

  Fragments arranged by size: 

                1624     730     611     190      93      74      62      62
            
 
BspEI T'CCGG_A

Cuts at:   2666    2666 
   Size:       3446
 
BspHI T'CATG_A

Cuts at:    417    2622     417 
   Size:       2205    1241
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    496     710    1012    1393     496 
   Size:        214     302     381    2549

  Fragments arranged by size: 

                2549     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1738    1792    3276    3441      50 
   Size:       1688      54    1484     165      55

  Fragments arranged by size: 

                1688    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1358    1358 
   Size:       3446
 
BsrFI r'CCGG_y

Cuts at:   1444    1625    1834    1444 
   Size:        181     209    3056

  Fragments arranged by size: 

                3056     209     181
 
BssSI C'ACGA_G

Cuts at:   1717    2075    1717 
   Size:        358    3088
 
BstAPI GCAn_nnn'nTGC

Cuts at:    150     823     150 
   Size:        673    2773
 
BstBI TT'CG_AA

Cuts at:   1807    1807 
   Size:       3446
 
BstYI r'GATC_y

Cuts at:      1     471     938     961    1296    1542    2554    2640    2652 
   Size:        470     467      23     335     246    1012      86      12

Cuts at:   2652    2998       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     470     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3446
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1396    1577    1740    1396 
   Size:        181     163    3102

  Fragments arranged by size: 

                3102     181     163
 
BtsI GCAGTG_nn'

Cuts at:    689     689 
   Size:       3446
 
DraI TTT'AAA

Cuts at:    445     445 
   Size:       3446
 
DrdI GACnn_nn'nnGTC

Cuts at:   1152    2010    1152 
   Size:        858    2588
 
EaeI y'GGCC_r

Cuts at:   1031    1205    1596    1623    2968    1031 
   Size:        174     391      27    1345    1509

  Fragments arranged by size: 

                1509    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1031    1031 
   Size:       3446
 
EarI CTCTTCn'nnn_

Cuts at:    128    1469    1679     128 
   Size:       1341     210    1895

  Fragments arranged by size: 

                1895    1341     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1654    1964    2110    3256    1654 
   Size:        310     146    1146    1844

  Fragments arranged by size: 

                1844    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    396     458     396 
   Size:         62    3384
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1271    1703    2450    2718    1271 
   Size:        432     747     268    1999

  Fragments arranged by size: 

                1999     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    179     179 
   Size:       3446
 
EcoO109I rG'GnC_Cy

Cuts at:    585     585 
   Size:       3446
 
EcoRI G'AATT_C

Cuts at:    450     450 
   Size:       3446
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1465    1465 
   Size:       3446
 
FspI TGC'GCA

Cuts at:   1227    1227 
   Size:       3446
 
HaeII r_GCGC'y

Cuts at:    880    1128    2150    3129     880 
   Size:        248    1022     979    1197

  Fragments arranged by size: 

                1197    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    357     389    1288    1318    1320    1350     357 
   Size:         32     899      30       2      30    2453

  Fragments arranged by size: 

                2453     899      32      30      30       2
 
HincII GTy'rAC

Cuts at:    485     485 
   Size:       3446
 
KasI G'GCGC_C

Cuts at:    876    1124     876 
   Size:        248    3198
 
KpnI G_GTAC'C

Cuts at:    466     466 
   Size:       3446
 
MluI A'CGCG_T

Cuts at:   2879    2879 
   Size:       3446
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2117    2301    2117 
   Size:        184    3262
 
MscI TGG'CCA

Cuts at:   1207    1207 
   Size:       3446
 
MslI CAynn'nnrTG

Cuts at:    419    1435    1562     419 
   Size:       1016     127    2303

  Fragments arranged by size: 

                2303    1016     127
 
MspA1I CmG'CkG

Cuts at:    557     871    1231    2244    2489     557 
   Size:        314     360    1013     245    1514

  Fragments arranged by size: 

                1514    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1627    1627 
   Size:       3446
 
NarI GG'CG_CC

Cuts at:    877    1125     877 
   Size:        248    3198
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3446
 
NgoMIV G'CCGG_C

Cuts at:   1625    1625 
   Size:       3446
 
NspI r_CATG'y

Cuts at:    499     999    1906     499 
   Size:        500     907    2039

  Fragments arranged by size: 

                2039     907     500
 
PciI A'CATG_T

Cuts at:   1902    1902 
   Size:       3446
 
PmeI GTTT'AAAC

Cuts at:    445     445 
   Size:       3446
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2585    2617    2585 
   Size:         32    3414
 
PstI C_TGCA'G

Cuts at:    207     362     493    1178     207 
   Size:        155     131     685    2475

  Fragments arranged by size: 

                2475     685     155     131
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3446
 
PvuII CAG'CTG

Cuts at:    871    1231     871 
   Size:        360    3086
 
RsrII CG'GwC_CG

Cuts at:   1641    1641 
   Size:       3446
 
SacI G_AGCT'C

Cuts at:    398     460     398 
   Size:         62    3384
 
SalI G'TCGA_C

Cuts at:    483     483 
   Size:       3446
 
SapI GCTCTTCn'nnn_

Cuts at:   1469    1679    1469 
   Size:        210    3236
 
SbfI CC_TGCA'GG

Cuts at:    207     493     207 
   Size:        286    3160
 
ScaI AGT'ACT

Cuts at:   2963    2963 
   Size:       3446
 
SfcI C'TryA_G

Cuts at:     33     203     358     489    1174    2167    2358      33 
   Size:        170     155     131     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     191     170     155     131
 
SfoI GGC'GCC

Cuts at:    878    1126     878 
   Size:        248    3198
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3446
 
SmaI CCC'GGG

Cuts at:    468     468 
   Size:       3446
 
SmlI C'TyrA_G

Cuts at:    601    1516    2008    2270     601 
   Size:        915     492     262    1777

  Fragments arranged by size: 

                1777     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3446
 
SphI G_CATG'C

Cuts at:    499     999     499 
   Size:        500    2946
 
SspI AAT'ATT

Cuts at:    117     314     117 
   Size:        197    3249
 
StyI C'CwwG_G

Cuts at:    107     580     934     107 
   Size:        473     354    2619

  Fragments arranged by size: 

                2619     473     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    277    1029    1162    1621    1830     277 
   Size:        752     133     459     209    1893

  Fragments arranged by size: 

                1893     752     459     209     133
 
TatI w'GTAC_w

Cuts at:   2961    2961 
   Size:       3446
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2963    2995    2963 
   Size:         32    3414
 
Tth111I GACn'n_nGTC

Cuts at:   1243    1243 
   Size:       3446
 
XbaI T'CTAG_A

Cuts at:    477     477 
   Size:       3446
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    941     941 
   Size:       3446
 
XmaI C'CCGG_G

Cuts at:    466     466 
   Size:       3446
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SspI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI     SwaI
     XhoI     XmnI     ZraI
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