Restriction map for pF1KB8736
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BbrPI       BbsI        BbvCI       
BcgI        BclI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmFI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HgaI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AciI          350  GCGG                 CCGC(-3/-1)
AcyI          659  GGCGCC               G[GA]^CG[CT]C
AlwI          529  GGATC                GGATC(4/5)
AlwNI         702  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         981  GAGCTC               G[AT]GC[AT]^C
AvaII          69  GGACC                G^G[AT]CC
BanI          659  GGCGCC               G^G[CT][GA]CC
BbeI          659  GGCGCC               GGCGC^C
BciVI         130  GTATCC               GTATCC(6/5)
BcnI          954  CCGGG                CC^[GC]GG
BfaI          127  CTAG                 C^TAG
BsaHI         659  GGCGCC               G[GA]^CG[CT]C
BsaJI         239  CCAAGG               C^C[ACGT][ACGT]GG
BsgI          829  CTGCAC               GTGCAG(16/14)
BsiHKAI       981  GAGCTC               G[AT]GC[AT]^C
BsmBI         818  CGTCTC               CGTCTC(1/5)
BstNI         285  CCAGG                CC^[AT]GG
BstYI         529  GGATCT               [GA]^GATC[CT]
Cac8I         346  GCCTGC               GC[ACGT]^[ACGT]GC
CfoI          660  GCGC                 GCG^C
EcoRII        285  CCAGG                ^CC[AT]GG
EcoT14I       239  CCAAGG               C^C[AT][AT]GG
HaeII         659  GGCGCC               [GA]GCGC^[CT]
HapII         954  CCGG                 C^CGG
HhaI          660  GCGC                 GCG^C
Hin1I         659  GGCGCC               G[GA]^CG[CT]C
HinP1I        660  GCGC                 G^CGC
HindIII       474  AAGCTT               A^AGCTT
HpaI          499  GTTAAC               GTT^AAC
HpaII         954  CCGG                 C^CGG
KasI          659  GGCGCC               G^GCGCC
MaeI          127  CTAG                 C^TAG
MflI          529  GGATCT               [GA]^GATC[CT]
MslI          513  CATTCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         49  CAGCTG               C[AC]G^C[GT]G
MspI          954  CCGG                 C^CGG
MvaI          285  CCAGG                CC^[AT]GG
NarI          659  GGCGCC               GG^CGCC
NciI          954  CCGGG                CC^[GC]GG
NdeI          140  CATATG               CA^TATG
PleI          924  GACTC                GAGTC(4/5)
PpuMI          68  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          322  CTGCAG               CTGCA^G
PvuII          49  CAGCTG               CAG^CTG
SacI          981  GAGCTC               GAGCT^C
SapI          713  GAAGAGC              GCTCTTC(1/4)
SfoI          659  GGCGCC               GGC^GCC
StyI          239  CCAAGG               C^C[AT][AT]GG
TaqI          331  TCGA                 T^CGA
TfiI          244  GAATC                G^A[AT]TC
TthHB8I       331  TCGA                 T^CGA
XhoII         529  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BalI          110  TGGCCA               TGG^CCA
              360  TGGCCA              
BsmAI         393  GAGAC                GTCTC(1/5)
              819  GTCTC               
BssKI         285  CCAGG                ^CC[ACGT]GG
              954  CCGGG               
DraII          68  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1135  AGGGCCT             
EaeI          110  TGGCCA               [CT]^GGCC[GA]
              360  TGGCCA              
EarI          222  GAAGAG               CTCTTC(1/4)
              713  GAAGAG              
EcoO109I       68  AGGACCT              [GA]G^G[ACGT]CC[CT]
             1135  AGGGCCT             
EcoRI          25  GAATTC               G^AATTC
              550  GAATTC              
HincII        411  GTCAAC               GT[CT]^[GA]AC
              499  GTTAAC              
HindII        411  GTCAAC               GT[CT]^[GA]AC
              499  GTTAAC              
HinfI         244  GAATC                G^A[ACGT]TC
              924  GACTC               
HphI           84  GGTGA                GGTGA(8/7)
              169  GGTGA               
Ksp632I       222  GAAGAG               CTCTTC(1/4)
              713  GAAGAG              
MluNI         110  TGGCCA               TGG^CCA
              360  TGGCCA              
MscI          110  TGGCCA               TGG^CCA
              360  TGGCCA              
MwoI          660  GCGCCATCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1144  GCTCATACTGC         
NlaIV         205  GGAGCC               GG[ACGT]^[ACGT]CC
              659  GGCGCC              
ScrFI         285  CCAGG                CC^[ACGT]GG
              954  CCGGG               


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AcsI        AluI        ApoI        BanII       BbvI        
BmyI        BpmI        BsiYI       BslI        BsmI        
Bsp1286I    BsrI        BstF5I      Cfr13I      DdeI        
DpnII       Fnu4HI      FokI        HaeIII      ItaI        
MaeIII      MboI        MboII       MnlI        MseI        
NdeII       NlaIII      Sau3AI      Sau96I      SfaNI       
SfcI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
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