################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BcnI BfrI BglI BglII BlnI BpuAI BsaAI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsoBI BspDI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV FauI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI ItaI KasI KpnI KspI MaeII MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI TseI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflIII 1113 ACATGT A^C[GA][CT]GT Alw44I 1783 GTGCAC G^TGCAC ApaLI 1783 GTGCAC G^TGCAC AspI 3 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 597 GGACC G^G[AT]CC BamHI 75 GGATCC G^GATCC BclI 858 TGATCA T^GATCA BlpI 1181 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1181 GCTCAGC GC^T[ACGT]AGC BsaBI 256 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 98 ACCGGA [AT]^CCGG[AT] BseRI 58 GAGGAG GAGGAG(10/8) BsmFI 9 GTCCC GGGAC(10/14) Bsp1407I 1194 TGTACA T^GTACA BspLU11I 1113 ACATGT A^CATGT BsrGI 1194 TGTACA T^GTACA BstAPI 1015 GCATCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 75 GGATCC [GA]^GATC[CT] CelII 1181 GCTCAGC GC^T[ACGT]AGC Cfr13I 597 GGACC G^G[ACGT]CC DsaI 196 CCATGG C^C[GA][CT]GG EcoT14I 196 CCATGG C^C[AT][AT]GG FbaI 858 TGATCA T^GATCA HaeIII 1795 GGCC GG^CC HapII 99 CCGG C^CGG HinfI 7 GAGTC G^A[ACGT]TC HpaII 99 CCGG C^CGG MamI 256 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 75 GGATCC [GA]^GATC[CT] MspI 99 CCGG C^CGG MwoI 1015 GCATCCCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 196 CCATGG C^CATGG NlaIV 75 GGATCC GG[ACGT]^[ACGT]CC PflFI 3 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 7 GAGTC GAGTC(4/5) SapI 1024 GCTCTTC GCTCTTC(1/4) Sau96I 597 GGACC G^G[ACGT]CC SspBI 1194 TGTACA T^GTACA StyI 196 CCATGG C^C[AT][AT]GG Tth111I 3 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 75 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 96 GTAC GT^AC 1195 GTAC AlwI 76 GATCC GGATC(4/5) 75 GGATC AspHI 1683 GTGCTC G[AT]GC[AT]^C 1783 GTGCAC BfaI 112 CTAG C^TAG 1298 CTAG BmyI 1683 GTGCTC G[AGT]GC[ACT]^C 1783 GTGCAC BsiHKAI 1683 GTGCTC G[AT]GC[AT]^C 1783 GTGCAC BsmAI 225 GAGAC GTCTC(1/5) 288 GAGAC Bsp1286I 1683 GTGCTC G[AGT]GC[ACT]^C 1783 GTGCAC BspHI 861 TCATGA T^CATGA 1449 TCATGA BssKI 12 CCAGG ^CC[ACGT]GG 273 CCAGG BstNI 12 CCAGG CC^[AT]GG 273 CCAGG DpnII 76 GATC ^GATC 859 GATC EarI 1025 CTCTTC CTCTTC(1/4) 66 GAAGAG EcoRII 12 CCAGG ^CC[AT]GG 273 CCAGG EcoT22I 809 ATGCAT ATGCA^T 977 ATGCAT HindIII 746 AAGCTT A^AGCTT 1334 AAGCTT Ksp632I 1025 CTCTTC CTCTTC(1/4) 66 GAAGAG MaeI 112 CTAG C^TAG 1298 CTAG MboI 76 GATC ^GATC 859 GATC MvaI 12 CCAGG CC^[AT]GG 273 CCAGG NdeII 76 GATC ^GATC 859 GATC NsiI 809 ATGCAT ATGCA^T 977 ATGCAT RcaI 861 TCATGA T^CATGA 1449 TCATGA RsaI 96 GTAC GT^AC 1195 GTAC Sau3AI 76 GATC ^GATC 859 GATC ScrFI 12 CCAGG CC^[ACGT]GG 273 CCAGG SfcI 654 CTGTAG C^T[GA][CT]AG 1905 CTGTAG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AcsI AluI ApoI Asp700I BpmI BsaJI BsmI BsrDI BsrI BstF5I DdeI EcoRI FokI HphI MaeIII MboII MnlI MseI MslI NdeI NlaIII NspI SfaNI TaqI Tru9I Tsp45I Tsp509I TspRI TthHB8I XmnI