(Circular) (Six-base) MAPSORT of: pF1KB8731.seq Check: 9513 from: 1 to: 5171 pF1KB8731 5171 bp With 182 enzymes: SgfI * June 20, 2008 10:29 .. AccI GT'mk_AC Cuts at: 2209 2209 Size: 5171 Acc65I G'GTAC_C Cuts at: 2187 2187 Size: 5171 AclI AA'CG_TT Cuts at: 5011 5011 Size: 5171 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 452 759 927 1347 1599 2996 3428 3781 4443 Size: 307 168 420 252 1397 432 353 662 Cuts at: 4443 452 Size: 1180 Fragments arranged by size: 1397 1180 662 432 420 353 307 252 168 AflIII A'CryG_T Cuts at: 1221 2523 4323 4604 1221 Size: 1302 1800 281 1788 Fragments arranged by size: 1800 1788 1302 281 AleI CACnn'nnGTG Cuts at: 1322 1826 1322 Size: 504 4667 AlwNI CAG_nnn'CTG Cuts at: 2476 3914 2476 Size: 1438 3733 ApaLI G'TGCA_C Cuts at: 1891 4009 1891 Size: 2118 3053 ApoI r'AATT_y Cuts at: 412 460 725 809 893 977 1229 1397 1481 Size: 48 265 84 84 84 252 168 84 Cuts at: 1481 1565 2153 2175 4524 4780 412 Size: 84 588 22 2349 256 803 Fragments arranged by size: 2349 803 588 265 256 252 168 84 84 84 84 84 48 22 AseI AT'TA_AT Cuts at: 20 20 Size: 5171 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5171 AvaI C'yCGr_G Cuts at: 2191 2191 Size: 5171 BamHI G'GATC_C Cuts at: 183 2196 183 Size: 2013 3158 BanI G'GyrC_C Cuts at: 2187 2601 2849 2884 2187 Size: 414 248 35 4474 Fragments arranged by size: 4474 414 248 35 BanII G_rGCy'C Cuts at: 2185 3215 2185 Size: 1030 4141 BbeI G_GCGC'C Cuts at: 2605 2853 2605 Size: 248 4923 BciVI GTATCCnnnnn_n' Cuts at: 3069 4125 5171 3069 Size: 1056 1046 3069 Fragments arranged by size: 3069 1056 1046 BclI T'GATC_A Cuts at: 966 2691 966 Size: 1725 3446 BfrBI ATG'CAT Cuts at: 919 1087 919 Size: 168 5003 BglII A'GATC_T Cuts at: 2686 4723 2686 Size: 2037 3134 BlpI GC'TnA_GC Cuts at: 1290 2283 1290 Size: 993 4178 Bme1580I G_kGCm'C Cuts at: 1895 2796 2889 4013 1895 Size: 901 93 1124 3053 Fragments arranged by size: 3053 1124 901 93 BmrI ACTGGGnnnn_n' Cuts at: 1079 1499 2383 2464 2799 4943 1079 Size: 420 884 81 335 2144 1307 Fragments arranged by size: 2144 1307 884 420 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3538 3538 Size: 5171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 755 923 1007 1595 1679 755 Size: 168 84 588 84 4247 Fragments arranged by size: 4247 588 168 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2347 3226 3940 4238 2347 Size: 879 714 298 3280 Fragments arranged by size: 3280 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 4875 4875 Size: 5171 BsaAI yAC'GTr Cuts at: 2524 2524 Size: 5171 BsaBI GATnn'nnATC Cuts at: 6 368 2685 4722 6 Size: 362 2317 2037 455 Fragments arranged by size: 2317 2037 455 362 BsaHI Gr'CG_yC Cuts at: 2602 2850 3552 5048 2602 Size: 248 702 1496 2725 Fragments arranged by size: 2725 1496 702 248 BsaWI w'CCGG_w Cuts at: 206 2360 2881 3559 3970 4117 4391 206 Size: 2154 521 678 411 147 274 986 Fragments arranged by size: 2154 986 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 181 181 Size: 5171 BseYI C'CCAG_C Cuts at: 2596 3354 4019 4427 2596 Size: 758 665 408 3340 Fragments arranged by size: 3340 758 665 408 BsiEI CG_ry'CG Cuts at: 104 2713 2759 3989 104 Size: 2609 46 1230 1286 Fragments arranged by size: 2609 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 1795 1895 2185 2963 3153 4013 1795 Size: 100 290 778 190 860 2953 Fragments arranged by size: 2953 860 778 290 190 100 BsmI GAATG_Cn' Cuts at: 1024 1354 1597 1024 Size: 330 243 4598 Fragments arranged by size: 4598 330 243 Bsp1286I G_dGCh'C Cuts at: 1795 1895 2185 2796 2889 2963 3153 3215 4013 Size: 100 290 611 93 74 190 62 798 Cuts at: 4013 1795 Size: 2953 Fragments arranged by size: 2953 798 611 290 190 100 93 74 62 BspHI T'CATG_A Cuts at: 969 1557 3603 969 Size: 588 2046 2537 Fragments arranged by size: 2537 2046 588 BspMI ACCTGCnnnn'nnnn_ Cuts at: 2221 2435 2737 3118 2221 Size: 214 302 381 4274 Fragments arranged by size: 4274 381 302 214 BsrBI CCG'CTC Cuts at: 50 3463 3517 5001 5166 50 Size: 3413 54 1484 165 55 Fragments arranged by size: 3413 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 450 1046 1550 1602 3083 450 Size: 596 504 52 1481 2538 Fragments arranged by size: 2538 1481 596 504 52 BsrFI r'CCGG_y Cuts at: 3169 3350 3169 Size: 181 4990 BsrGI T'GTAC_A Cuts at: 1302 1302 Size: 5171 BssSI C'ACGA_G Cuts at: 3442 4150 3442 Size: 708 4463 BstAPI GCAn_nnn'nTGC Cuts at: 1129 2548 1129 Size: 1419 3752 BstBI TT'CG_AA Cuts at: 3532 3532 Size: 5171 BstYI r'GATC_y Cuts at: 1 183 2196 2663 2686 3021 3267 3573 3585 Size: 182 2013 467 23 335 246 306 12 Cuts at: 3585 3671 4723 1 Size: 86 1052 449 Fragments arranged by size: 2013 1052 467 449 335 306 246 182 86 23 12 BtgI C'CryG_G Cuts at: 304 304 Size: 5171 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3121 3302 3465 3121 Size: 181 163 4827 Fragments arranged by size: 4827 181 163 BtsI GCAGTG_nn' Cuts at: 1795 2414 1795 Size: 619 4552 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1584 1619 1584 Size: 35 5136 DraI TTT'AAA Cuts at: 2170 2170 Size: 5171 DrdI GACnn_nn'nnGTC Cuts at: 2877 4221 2877 Size: 1344 3827 EaeI y'GGCC_r Cuts at: 2756 2930 3321 3348 4693 2756 Size: 174 391 27 1345 3234 Fragments arranged by size: 3234 1345 391 174 27 EagI C'GGCC_G Cuts at: 2756 2756 Size: 5171 EarI CTCTTCn'nnn_ Cuts at: 169 1139 3194 3404 169 Size: 970 2055 210 1936 Fragments arranged by size: 2055 1936 970 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3379 4121 4267 4981 3379 Size: 742 146 714 3569 Fragments arranged by size: 3569 742 714 146 EcoICRI GAG'CTC Cuts at: 2183 2183 Size: 5171 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 452 755 759 923 927 1007 1347 1595 1599 Size: 303 4 164 4 80 340 248 4 Cuts at: 1599 1679 2996 3428 3781 4443 452 Size: 80 1317 432 353 662 1180 Fragments arranged by size: 1317 1180 662 432 353 340 303 248 164 80 80 4 4 4 EcoO109I rG'GnC_Cy Cuts at: 2310 2310 Size: 5171 EcoRI G'AATT_C Cuts at: 725 893 1229 1481 2153 2175 725 Size: 168 336 252 672 22 3721 Fragments arranged by size: 3721 672 336 252 168 22 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1395 1647 3190 1395 Size: 252 1543 3376 Fragments arranged by size: 3376 1543 252 FspI TGC'GCA Cuts at: 2952 2952 Size: 5171 HaeII r_GCGC'y Cuts at: 2605 2853 4083 4854 2605 Size: 248 1230 771 2922 Fragments arranged by size: 2922 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 168 200 497 529 1446 1478 3013 3043 3045 Size: 32 297 32 917 32 1535 30 2 Cuts at: 3045 3075 168 Size: 30 2264 Fragments arranged by size: 2264 1535 917 297 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 2210 2210 Size: 5171 HindIII A'AGCT_T Cuts at: 854 1442 854 Size: 588 4583 KasI G'GCGC_C Cuts at: 2601 2849 2601 Size: 248 4923 KpnI G_GTAC'C Cuts at: 2191 2191 Size: 5171 MluI A'CGCG_T Cuts at: 4604 4604 Size: 5171 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3930 4114 3930 Size: 184 4987 MscI TGG'CCA Cuts at: 2932 2932 Size: 5171 MslI CAynn'nnrTG Cuts at: 569 865 1238 1322 1826 1910 1994 2078 2162 Size: 296 373 84 504 84 84 84 84 Cuts at: 2162 3160 3287 569 Size: 998 127 2453 Fragments arranged by size: 2453 998 504 373 296 127 84 84 84 84 84 MspA1I CmG'CkG Cuts at: 2282 2596 2956 3740 3985 2282 Size: 314 360 784 245 3468 Fragments arranged by size: 3468 784 360 314 245 NaeI GCC'GGC Cuts at: 3352 3352 Size: 5171 NarI GG'CG_CC Cuts at: 2602 2850 2602 Size: 248 4923 NcoI C'CATG_G Cuts at: 304 304 Size: 5171 NdeI CA'TA_TG Cuts at: 627 1139 1307 1811 2063 627 Size: 512 168 504 252 3735 Fragments arranged by size: 3735 512 504 252 168 NgoMIV G'CCGG_C Cuts at: 3350 3350 Size: 5171 NsiI A_TGCA'T Cuts at: 921 1089 921 Size: 168 5003 NspI r_CATG'y Cuts at: 923 1091 1225 2224 2724 4327 923 Size: 168 134 999 500 1603 1767 Fragments arranged by size: 1767 1603 999 500 168 134 PasI CC'CwG_GG Cuts at: 120 120 Size: 5171 PciI A'CATG_T Cuts at: 1221 4323 1221 Size: 3102 2069 PmeI GTTT'AAAC Cuts at: 2170 2170 Size: 5171 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3617 3649 3617 Size: 32 5139 PsiI TTA'TAA Cuts at: 806 1169 806 Size: 363 4808 PstI C_TGCA'G Cuts at: 2218 2903 2218 Size: 685 4486 PvuI CG_AT'CG Cuts at: 104 104 Size: 5171 PvuII CAG'CTG Cuts at: 2596 2956 2596 Size: 360 4811 RsrII CG'GwC_CG Cuts at: 3366 3366 Size: 5171 SacI G_AGCT'C Cuts at: 2185 2185 Size: 5171 SalI G'TCGA_C Cuts at: 2208 2208 Size: 5171 SapI GCTCTTCn'nnn_ Cuts at: 1139 3194 3404 1139 Size: 2055 210 2906 Fragments arranged by size: 2906 2055 210 SbfI CC_TGCA'GG Cuts at: 2218 2218 Size: 5171 ScaI AGT'ACT Cuts at: 4688 4688 Size: 5171 SfcI C'TryA_G Cuts at: 33 762 2013 2214 2899 3867 4058 33 Size: 729 1251 201 685 968 191 1146 Fragments arranged by size: 1251 1146 968 729 685 201 191 SfoI GGC'GCC Cuts at: 2603 2851 2603 Size: 248 4923 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5171 SmaI CCC'GGG Cuts at: 2193 2193 Size: 5171 SmlI C'TyrA_G Cuts at: 2326 3241 3955 4217 2326 Size: 915 714 262 3280 Fragments arranged by size: 3280 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5171 SphI G_CATG'C Cuts at: 2224 2724 2224 Size: 500 4671 StyI C'CwwG_G Cuts at: 304 2305 2659 304 Size: 2001 354 2816 Fragments arranged by size: 2816 2001 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 297 2754 2887 3346 3555 297 Size: 2457 133 459 209 1913 Fragments arranged by size: 2457 1913 459 209 133 TatI w'GTAC_w Cuts at: 1302 4686 1302 Size: 3384 1787 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4688 4720 4688 Size: 32 5139 Tth111I GACn'n_nGTC Cuts at: 114 2968 114 Size: 2854 2317 XbaI T'CTAG_A Cuts at: 2202 2202 Size: 5171 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2666 2666 Size: 5171 XmaI C'CCGG_G Cuts at: 2191 2191 Size: 5171 XmnI GAAnn'nnTTC Cuts at: 940 977 1378 1565 1864 2005 940 Size: 37 401 187 299 141 4106 Fragments arranged by size: 4106 401 299 187 141 37 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BspEI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NheI NotI NruI PacI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI