Restriction map for pF1KB8729
 (Linear) (Six-base) MAPSORT of: KIBB8729.seq  Check: 6417  from: 1  to: 2523



KIBB8729.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      16     262     582     867    1427    2351    2523 
   Size:         16     246     320     285     560     924     172

  Fragments arranged by size: 

                 924     560     320     285     246     172      16
 
AatII G_ACGT'C

Cuts at:      0     604    1318    1769    2523 
   Size:        604     714     451     754

  Fragments arranged by size: 

                 754     714     604     451
 
AccI GT'mk_AC

Cuts at:      0    1769    2026    2523 
   Size:       1769     257     497

  Fragments arranged by size: 

                1769     497     257
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     598    1699    2023    2523 
   Size:        598    1101     324     500

  Fragments arranged by size: 

                1101     598     500     324
 
AfeI AGC'GCT

Cuts at:      0    2464    2523 
   Size:       2464      59
 
AflIII A'CryG_T

Cuts at:      0     866    2523 
   Size:        866    1657
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1050    2055    2523 
   Size:       1050    1005     468

  Fragments arranged by size: 

                1050    1005     468
 
AleI CACnn'nnGTG

Cuts at:      0     416    2443    2523 
   Size:        416    2027      80

  Fragments arranged by size: 

                2027     416      80
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2077    2523 
   Size:       2077     446
 
AlwNI CAG_nnn'CTG

Cuts at:      0     171     597    1506    1839    2523 
   Size:        171     426     909     333     684

  Fragments arranged by size: 

                 909     684     426     333     171
 
ApaI G_GGCC'C

Cuts at:      0    1279    2113    2319    2523 
   Size:       1279     834     206     204

  Fragments arranged by size: 

                1279     834     206     204
 
ApaLI G'TGCA_C

Cuts at:      0    1180    2523 
   Size:       1180    1343
 
ApoI r'AATT_y

Cuts at:      0    1807    2523 
   Size:       1807     716
 
AvaI C'yCGr_G

Cuts at:      0     479     860    1138    1230    2523 
   Size:        479     381     278      92    1293

  Fragments arranged by size: 

                1293     479     381     278      92
 
BamHI G'GATC_C

Cuts at:      0    1019    1574    2523 
   Size:       1019     555     949

  Fragments arranged by size: 

                1019     949     555
 
BanI G'GyrC_C

Cuts at:      0      96     451    1415    1945    2262    2505    2523 
   Size:         96     355     964     530     317     243      18

  Fragments arranged by size: 

                 964     530     355     317     243      96      18
 
BanII G_rGCy'C

Cuts at:      0     361    1279    1726    2113    2319    2495    2523 
   Size:        361     918     447     387     206     176      28

  Fragments arranged by size: 

                 918     447     387     361     206     176      28
 
BbeI G_GCGC'C

Cuts at:      0     455    2266    2523 
   Size:        455    1811     257

  Fragments arranged by size: 

                1811     455     257
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1203    2523 
   Size:       1203    1320
 
BbvCI CC'TCA_GC

Cuts at:      0    1915    2512    2523 
   Size:       1915     597      11

  Fragments arranged by size: 

                1915     597      11
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2224    2258    2523 
   Size:       2224      34     265

  Fragments arranged by size: 

                2224     265      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2034    2523 
   Size:       2034     489
 
BclI T'GATC_A

Cuts at:      0    1787    2523 
   Size:       1787     736
 
BglI GCCn_nnn'nGGC

Cuts at:      0     588    1588    2523 
   Size:        588    1000     935

  Fragments arranged by size: 

                1000     935     588
 
BglII A'GATC_T

Cuts at:      0     971    1745    2523 
   Size:        971     774     778

  Fragments arranged by size: 

                 971     778     774
 
BlpI GC'TnA_GC

Cuts at:      0    1354    2523 
   Size:       1354    1169
 
Bme1580I G_kGCm'C

Cuts at:      0     101    1184    1279    2113    2319    2510    2523 
   Size:        101    1083      95     834     206     191      13

  Fragments arranged by size: 

                1083     834     206     191     101      95      13
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1484    2523 
   Size:       1484    1039
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     492    2523 
   Size:        492    2031
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     342     441    1414    1453    2523 
   Size:        280      62      99     973      39    1070

  Fragments arranged by size: 

                1070     973     280      99      62      39
 
Bpu10I CC'TnA_GC

Cuts at:      0    1915    2512    2523 
   Size:       1915     597      11

  Fragments arranged by size: 

                1915     597      11
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     832    1087    1780    2404    2523 
   Size:        832     255     693     624     119

  Fragments arranged by size: 

                 832     693     624     255     119
 
BsaAI yAC'GTr

Cuts at:      0     438    1482    2523 
   Size:        438    1044    1041

  Fragments arranged by size: 

                1044    1041     438
 
BsaBI GATnn'nnATC

Cuts at:      0     187    2523 
   Size:        187    2336
 
BsaHI Gr'CG_yC

Cuts at:      0     452     601    1315    1766    2263    2523 
   Size:        452     149     714     451     497     260

  Fragments arranged by size: 

                 714     497     452     451     260     149
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      55      85     910     940    2523 
   Size:         55      30     825      30    1583

  Fragments arranged by size: 

                1583     825      55      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1540    2523 
   Size:       1540     983
 
BseYI C'CCAG_C

Cuts at:      0     350     583    1953    2008    2112    2161    2179    2523 
   Size:        350     233    1370      55     104      49      18     344

  Fragments arranged by size: 

                1370     350     344     233     104      55      49      18
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     394     418     736     783    1462    1504    1599 
   Size:        202     192      24     318      47     679      42      95

Cuts at:   1599    1734    2338    2376    2523 
   Size:        135     604      38     147

  Fragments arranged by size: 

                 679     604     318     202     192     147     135      95
                  47      42      38      24
 
BsiEI CG_ry'CG

Cuts at:      0     633    1058    1734    2523 
   Size:        633     425     676     789

  Fragments arranged by size: 

                 789     676     633     425
 
BsiHKAI G_wGCw'C

Cuts at:      0      82     206     239     361    1184    1364    2523 
   Size:         82     124      33     122     823     180    1159

  Fragments arranged by size: 

                1159     823     180     124     122      82      33
 
BsmI GAATG_Cn'

Cuts at:      0    2152    2386    2523 
   Size:       2152     234     137

  Fragments arranged by size: 

                2152     234     137
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     101     206     239     361    1184    1279    1364 
   Size:         82      19     105      33     122     823      95      85

Cuts at:   1364    1726    2113    2319    2495    2510    2523 
   Size:        362     387     206     176      15      13

  Fragments arranged by size: 

                 823     387     362     206     176     122     105      95
                  85      82      33      19      15      13
 
BspHI T'CATG_A

Cuts at:      0    1784    2523 
   Size:       1784     739
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      16     158     224     262     582     867    1427    1548 
   Size:         16     142      66      38     320     285     560     121

Cuts at:   1548    1889    2351    2523 
   Size:        341     462     172

  Fragments arranged by size: 

                 560     462     341     320     285     172     142     121
                  66      38      16
 
BsrFI r'CCGG_y

Cuts at:      0     620     661    1184    1248    1450    1733    2523 
   Size:        620      41     523      64     202     283     790

  Fragments arranged by size: 

                 790     620     523     283     202      64      41
 
BssSI C'ACGA_G

Cuts at:      0     241    1522    2523 
   Size:        241    1281    1001

  Fragments arranged by size: 

                1281    1001     241
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      86    1839    1851    2390    2399    2523 
   Size:         86    1753      12     539       9     124

  Fragments arranged by size: 

                1753     539     124      86      12       9
 
BstEII G'GTnAC_C

Cuts at:      0     561    1446    1620    2523 
   Size:        561     885     174     903

  Fragments arranged by size: 

                 903     885     561     174
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1041    2523 
   Size:       1041    1482
 
BstYI r'GATC_y

Cuts at:      0     773     971    1019    1574    1745    2090    2523 
   Size:        773     198      48     555     171     345     433

  Fragments arranged by size: 

                 773     555     433     345     198     171      48
 
BstZ17I GTA'TAC

Cuts at:      0    2027    2523 
   Size:       2027     496
 
BtgI C'CryG_G

Cuts at:      0     143     524     686     980    1058    1202    1223    1271 
   Size:        143     381     162     294      78     144      21      48

Cuts at:   1271    1344    1379    2372    2438    2523 
   Size:         73      35     993      66      85

  Fragments arranged by size: 

                 993     381     294     162     144     143      85      78
                  73      66      48      35      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     117     173     636    1644    2117    2523 
   Size:        117      56     463    1008     473     406

  Fragments arranged by size: 

                1008     473     463     406     117      56
 
BtsI GCAGTG_nn'

Cuts at:      0      84    1192    1828    1861    2286    2458    2523 
   Size:         84    1108     636      33     425     172      65

  Fragments arranged by size: 

                1108     636     425     172      84      65      33
 
DraIII CAC_nnn'GTG

Cuts at:      0    1179    2399    2523 
   Size:       1179    1220     124

  Fragments arranged by size: 

                1220    1179     124
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1168    2523 
   Size:       1168    1355
 
EaeI y'GGCC_r

Cuts at:      0      16     630     644     683    1028    1055    1109    1205 
   Size:         16     614      14      39     345      27      54      96

Cuts at:   1205    1250    1580    1886    2030    2256    2523 
   Size:         45     330     306     144     226     267

  Fragments arranged by size: 

                 614     345     330     306     267     226     144      96
                  54      45      39      27      16      14
 
EagI C'GGCC_G

Cuts at:      0     630    1055    2523 
   Size:        630     425    1468

  Fragments arranged by size: 

                1468     630     425
 
EarI CTCTTCn'nnn_

Cuts at:      0    1429    1657    2071    2523 
   Size:       1429     228     414     452

  Fragments arranged by size: 

                1429     452     414     228
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     346     706    1032    1354    2059    2523 
   Size:        346     360     326     322     705     464

  Fragments arranged by size: 

                 705     464     360     346     326     322
 
EcoICRI GAG'CTC

Cuts at:      0     359    2523 
   Size:        359    2164
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     342     441     598    1414    1453    1699    2023 
   Size:        280      62      99     157     816      39     246     324

Cuts at:   2023    2523 
   Size:        500

  Fragments arranged by size: 

                 816     500     324     280     246     157      99      62
                  39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     376     934    1555    2394    2523 
   Size:        376     558     621     839     129

  Fragments arranged by size: 

                 839     621     558     376     129
 
EcoO109I rG'GnC_Cy

Cuts at:      0     168     213     384    1276    1560    1995    2109    2315 
   Size:        168      45     171     892     284     435     114     206

Cuts at:   2315    2316    2523 
   Size:          1     207

  Fragments arranged by size: 

                 892     435     284     207     206     171     168     114
                  45       1
 
FspI TGC'GCA

Cuts at:      0     154     934    2523 
   Size:        154     780    1589

  Fragments arranged by size: 

                1589     780     154
 
HaeII r_GCGC'y

Cuts at:      0     455    1066    1263    1411    1840    2137    2266    2366 
   Size:        455     611     197     148     429     297     129     100

Cuts at:   2366    2466    2523 
   Size:        100      57

  Fragments arranged by size: 

                 611     455     429     297     197     148     129     100
                 100      57
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     205     237     910     942    1036    1068 
   Size:         55      32     118      32     673      32      94      32

Cuts at:   1068    1933    1965    2041    2073    2082    2114    2523 
   Size:        865      32      76      32       9      32     409

  Fragments arranged by size: 

                 865     673     409     118      94      76      55      32
                  32      32      32      32      32      32       9
 
HincII GTy'rAC

Cuts at:      0    1770    2523 
   Size:       1770     753
 
KasI G'GCGC_C

Cuts at:      0     451    2262    2523 
   Size:        451    1811     261

  Fragments arranged by size: 

                1811     451     261
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     851    2523 
   Size:        851    1672
 
MscI TGG'CCA

Cuts at:      0     685    1111    1582    1888    2523 
   Size:        685     426     471     306     635

  Fragments arranged by size: 

                 685     635     471     426     306
 
MslI CAynn'nnrTG

Cuts at:      0     416     679    2443    2523 
   Size:        416     263    1764      80

  Fragments arranged by size: 

                1764     416     263      80
 
MspA1I CmG'CkG

Cuts at:      0      22     554     587    1225    1273    1392    1546    2012 
   Size:         22     532      33     638      48     119     154     466

Cuts at:   2012    2169    2523 
   Size:        157     354

  Fragments arranged by size: 

                 638     532     466     354     157     154     119      48
                  33      22
 
NaeI GCC'GGC

Cuts at:      0     622    1250    2523 
   Size:        622     628    1273

  Fragments arranged by size: 

                1273     628     622
 
NarI GG'CG_CC

Cuts at:      0     452    2263    2523 
   Size:        452    1811     260

  Fragments arranged by size: 

                1811     452     260
 
NcoI C'CATG_G

Cuts at:      0     143     524    1202    1379    2523 
   Size:        143     381     678     177    1144

  Fragments arranged by size: 

                1144     678     381     177     143
 
NgoMIV G'CCGG_C

Cuts at:      0     620    1248    2523 
   Size:        620     628    1275

  Fragments arranged by size: 

                1275     628     620
 
NotI GC'GGCC_GC

Cuts at:      0     630    2523 
   Size:        630    1893
 
NruI TCG'CGA

Cuts at:      0    2102    2523 
   Size:       2102     421
 
NspI r_CATG'y

Cuts at:      0     870    1962    2058    2523 
   Size:        870    1092      96     465

  Fragments arranged by size: 

                1092     870     465      96
 
PasI CC'CwG_GG

Cuts at:      0     209    2523 
   Size:        209    2314
 
PciI A'CATG_T

Cuts at:      0     866    2523 
   Size:        866    1657
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     192    1392    2087    2523 
   Size:        192    1200     695     436

  Fragments arranged by size: 

                1200     695     436     192
 
PfoI T'CCnGG_A

Cuts at:      0     191    2523 
   Size:        191    2332
 
PmlI CAC'GTG

Cuts at:      0     438    1482    2523 
   Size:        438    1044    1041

  Fragments arranged by size: 

                1044    1041     438
 
PpuMI rG'GwC_Cy

Cuts at:      0     168     213     384    1560    2523 
   Size:        168      45     171    1176     963

  Fragments arranged by size: 

                1176     963     171     168      45
 
PspOMI G'GGCC_C

Cuts at:      0    1275    2109    2315    2523 
   Size:       1275     834     206     208

  Fragments arranged by size: 

                1275     834     208     206
 
PstI C_TGCA'G

Cuts at:      0    1199    1556    1835    1844    2228    2252    2297    2523 
   Size:       1199     357     279       9     384      24      45     226

  Fragments arranged by size: 

                1199     384     357     279     226      45      24       9
            
 
PvuII CAG'CTG

Cuts at:      0    2012    2169    2523 
   Size:       2012     157     354

  Fragments arranged by size: 

                2012     354     157
 
SacI G_AGCT'C

Cuts at:      0     361    2523 
   Size:        361    2162
 
SacII CC_GC'GG

Cuts at:      0    1226    1274    2523 
   Size:       1226      48    1249

  Fragments arranged by size: 

                1249    1226      48
 
SalI G'TCGA_C

Cuts at:      0    1768    2523 
   Size:       1768     755
 
SanDI GG'GwC_CC

Cuts at:      0     384    2523 
   Size:        384    2139
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1429    1657    2071    2523 
   Size:       1429     228     414     452

  Fragments arranged by size: 

                1429     452     414     228
 
ScaI AGT'ACT

Cuts at:      0    1438    2523 
   Size:       1438    1085
 
SexAI A'CCwGG_T

Cuts at:      0    2395    2523 
   Size:       2395     128
 
SfcI C'TryA_G

Cuts at:      0    1195    1215    1552    1831    1840    2224    2248    2293 
   Size:       1195      20     337     279       9     384      24      45

Cuts at:   2293    2523 
   Size:        230

  Fragments arranged by size: 

                1195     384     337     279     230      45      24      20
                   9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1588    2523 
   Size:       1588     935
 
SfoI GGC'GCC

Cuts at:      0     453    2264    2523 
   Size:        453    1811     259

  Fragments arranged by size: 

                1811     453     259
 
SgrAI Cr'CCGG_yG

Cuts at:      0     661    2523 
   Size:        661    1862
 
SmaI CCC'GGG

Cuts at:      0     862    2523 
   Size:        862    1661
 
SmlI C'TyrA_G

Cuts at:      0     847    1102    1795    2419    2523 
   Size:        847     255     693     624     104

  Fragments arranged by size: 

                 847     693     624     255     104
 
SphI G_CATG'C

Cuts at:      0    1962    2523 
   Size:       1962     561
 
StuI AGG'CCT

Cuts at:      0    1236    1591    2389    2523 
   Size:       1236     355     798     134

  Fragments arranged by size: 

                1236     798     355     134
 
StyI C'CwwG_G

Cuts at:      0     143     524    1202    1379    2358    2523 
   Size:        143     381     678     177     979     165

  Fragments arranged by size: 

                 979     678     381     177     165     143
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      97     856    1756    1848    2523 
   Size:         97     759     900      92     675

  Fragments arranged by size: 

                 900     759     675      97      92
 
TatI w'GTAC_w

Cuts at:      0    1436    1529    2523 
   Size:       1436      93     994

  Fragments arranged by size: 

                1436     994      93
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     935     967    2523 
   Size:        935      32    1556

  Fragments arranged by size: 

                1556     935      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1780    2523 
   Size:       1780     743
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     255     912    1038    1575    2208    2523 
   Size:        255     657     126     537     633     315

  Fragments arranged by size: 

                 657     633     537     315     255     126
 
XmaI C'CCGG_G

Cuts at:      0     860    2523 
   Size:        860    1663
 
ZraI GAC'GTC

Cuts at:      0     602    1316    1767    2523 
   Size:        602     714     451     756

  Fragments arranged by size: 

                 756     714     602     451
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrFI    BssSI   BstAPI
   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FspI    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NruI
     NspI     PasI     PciI    PflMI     PfoI     PmlI    PpuMI   PspOMI
     PstI    PvuII     SacI    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SfcI     SfiI     SfoI    SgrAI     SmaI     SmlI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII     AgeI     AloI     AscI     AseI    AsiSI
    AvrII     BaeI    BfrBI    BmgBI     BmtI     BsaI    BsaWI    BsiWI
    BsmBI    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BstBI   Bsu36I
     ClaI    CspCI     DraI    EcoRI    EcoRV     FalI     FseI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NsiI
     PacI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SbfI     SgfI    SnaBI     SpeI     SrfI     SspI     SwaI
     XbaI     XhoI     XmnI
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