Restriction map for
pF1KB8717
(Circular) (Six-base) MAPSORT of: pF1KB8717.seq Check: 6467 from: 1 to: 4556
pF1KB8717 4556 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1028 1028
Size: 4556
AccI GT'mk_AC
Cuts at: 741 1594 741
Size: 853 3703
Acc65I G'GTAC_C
Cuts at: 1572 1572
Size: 4556
AclI AA'CG_TT
Cuts at: 485 4396 485
Size: 3911 645
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 567 913 2381 2813 3166 3828 567
Size: 346 1468 432 353 662 1295
Fragments arranged by size:
1468 1295 662 432 353 346
AflII C'TTAA_G
Cuts at: 375 375
Size: 4556
AflIII A'CryG_T
Cuts at: 1908 3708 3989 1908
Size: 1800 281 2475
Fragments arranged by size:
2475 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 504 504
Size: 4556
AleI CACnn'nnGTG
Cuts at: 1294 1294
Size: 4556
AlwNI CAG_nnn'CTG
Cuts at: 303 1221 1861 3299 303
Size: 918 640 1438 1560
Fragments arranged by size:
1560 1438 918 640
ApaLI G'TGCA_C
Cuts at: 3394 3394
Size: 4556
ApoI r'AATT_y
Cuts at: 1560 3909 4165 1560
Size: 2349 256 1951
Fragments arranged by size:
2349 1951 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4556
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
AvaI C'yCGr_G
Cuts at: 774 1292 1405 1576 774
Size: 518 113 171 3754
Fragments arranged by size:
3754 518 171 113
BamHI G'GATC_C
Cuts at: 915 1581 915
Size: 666 3890
BanI G'GyrC_C
Cuts at: 1572 1986 2234 2269 1572
Size: 414 248 35 3859
Fragments arranged by size:
3859 414 248 35
BanII G_rGCy'C
Cuts at: 1514 1570 2600 1514
Size: 56 1030 3470
Fragments arranged by size:
3470 1030 56
BbeI G_GCGC'C
Cuts at: 1990 2238 1990
Size: 248 4308
BbvCI CC'TCA_GC
Cuts at: 1505 1505
Size: 4556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 219 253 219
Size: 34 4522
BciVI GTATCCnnnnn_n'
Cuts at: 295 1111 2454 3510 4556 295
Size: 816 1343 1056 1046 295
Fragments arranged by size:
1343 1056 1046 816 295
BclI T'GATC_A
Cuts at: 2076 2076
Size: 4556
BfrBI ATG'CAT
Cuts at: 841 841
Size: 4556
BglII A'GATC_T
Cuts at: 318 2071 4108 318
Size: 1753 2037 766
Fragments arranged by size:
2037 1753 766
BlpI GC'TnA_GC
Cuts at: 1668 1668
Size: 4556
Bme1580I G_kGCm'C
Cuts at: 2181 2274 3398 2181
Size: 93 1124 3339
Fragments arranged by size:
3339 1124 93
BmgBI CAC'GTC
Cuts at: 174 174
Size: 4556
BmrI ACTGGGnnnn_n'
Cuts at: 1520 1768 1849 2184 4328 1520
Size: 248 81 335 2144 1748
Fragments arranged by size:
2144 1748 335 248 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1530 2923 1530
Size: 1393 3163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 313 1015 1305 150
Size: 163 702 290 3401
Fragments arranged by size:
3401 702 290 163
Bpu10I CC'TnA_GC
Cuts at: 193 1370 1505 193
Size: 1177 135 3244
Fragments arranged by size:
3244 1177 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 1732 2611 3325 3623 784
Size: 948 879 714 298 1717
Fragments arranged by size:
1717 948 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 403 1417 4260 403
Size: 1014 2843 699
Fragments arranged by size:
2843 1014 699
BsaAI yAC'GTr
Cuts at: 1909 1909
Size: 4556
BsaBI GATnn'nnATC
Cuts at: 6 2070 4107 6
Size: 2064 2037 455
Fragments arranged by size:
2064 2037 455
BsaHI Gr'CG_yC
Cuts at: 272 1987 2235 2937 4433 272
Size: 1715 248 702 1496 395
Fragments arranged by size:
1715 1496 702 395 248
BsaWI w'CCGG_w
Cuts at: 280 1745 2266 2944 3355 3502 3776 280
Size: 1465 521 678 411 147 274 1060
Fragments arranged by size:
1465 1060 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 623 205
Size: 418 4138
BseYI C'CCAG_C
Cuts at: 807 881 934 938 1981 2739 3404 3812 807
Size: 74 53 4 1043 758 665 408 1551
Fragments arranged by size:
1551 1043 758 665 408 74 53 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 735
Size: 4556
BsiEI CG_ry'CG
Cuts at: 104 251 2098 2144 3374 104
Size: 147 1847 46 1230 1286
Fragments arranged by size:
1847 1286 1230 147 46
BsiHKAI G_wGCw'C
Cuts at: 203 1570 2348 2538 3398 203
Size: 1367 778 190 860 1361
Fragments arranged by size:
1367 1361 860 778 190
BsmI GAATG_Cn'
Cuts at: 841 841
Size: 4556
BsmBI CGTCTCn'nnnn_
Cuts at: 1030 1030
Size: 4556
Bsp1286I G_dGCh'C
Cuts at: 203 1514 1570 2181 2274 2348 2538 2600 3398
Size: 1311 56 611 93 74 190 62 798
Cuts at: 3398 203
Size: 1361
Fragments arranged by size:
1361 1311 798 611 190 93 74 62
56
BspHI T'CATG_A
Cuts at: 2988 2988
Size: 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 297 1028 1503 1606 1820 2122 2503 297
Size: 731 475 103 214 302 381 2350
Fragments arranged by size:
2350 731 475 381 302 214 103
BsrBI CCG'CTC
Cuts at: 50 2848 2902 4386 4551 50
Size: 2798 54 1484 165 55
Fragments arranged by size:
2798 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1202 2468 1202
Size: 1266 3290
BsrFI r'CCGG_y
Cuts at: 2554 2735 2554
Size: 181 4375
BssHII G'CGCG_C
Cuts at: 226 226
Size: 4556
BssSI C'ACGA_G
Cuts at: 2827 3535 2827
Size: 708 3848
BstAPI GCAn_nnn'nTGC
Cuts at: 303 1133 1933 303
Size: 830 800 2926
Fragments arranged by size:
2926 830 800
BstBI TT'CG_AA
Cuts at: 2917 2917
Size: 4556
BstEII G'GTnAC_C
Cuts at: 1272 1272
Size: 4556
BstYI r'GATC_y
Cuts at: 1 318 795 915 1581 2048 2071 2406 2652
Size: 317 477 120 666 467 23 335 246
Cuts at: 2652 2958 2970 3056 4108 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 666 477 467 449 335 317 306
246 120 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 920 1456 920
Size: 536 4020
BtgI C'CryG_G
Cuts at: 1444 1444
Size: 4556
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2506 2687 2850 2506
Size: 181 163 4212
Fragments arranged by size:
4212 181 163
BtsI GCAGTG_nn'
Cuts at: 261 1273 1799 261
Size: 1012 526 3018
Fragments arranged by size:
3018 1012 526
DraI TTT'AAA
Cuts at: 1555 1555
Size: 4556
DraIII CAC_nnn'GTG
Cuts at: 1093 1093
Size: 4556
DrdI GACnn_nn'nnGTC
Cuts at: 2262 3606 2262
Size: 1344 3212
EaeI y'GGCC_r
Cuts at: 248 416 2141 2315 2706 2733 4078 248
Size: 168 1725 174 391 27 1345 726
Fragments arranged by size:
1725 1345 726 391 174 168 27
EagI C'GGCC_G
Cuts at: 248 2141 248
Size: 1893 2663
EarI CTCTTCn'nnn_
Cuts at: 851 1537 2579 2789 851
Size: 686 1042 210 2618
Fragments arranged by size:
2618 1042 686 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2764 3506 3652 4366 2764
Size: 742 146 714 2954
Fragments arranged by size:
2954 742 714 146
EcoICRI GAG'CTC
Cuts at: 1568 1568
Size: 4556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 313 567 913 1015 1305 2381 2813 3166
Size: 163 254 346 102 290 1076 432 353
Cuts at: 3166 3828 150
Size: 662 878
Fragments arranged by size:
1076 878 662 432 353 346 290 254
163 102
EcoNI CCTnn'n_nnAGG
Cuts at: 391 391
Size: 4556
EcoO109I rG'GnC_Cy
Cuts at: 309 878 1325 1695 309
Size: 569 447 370 3170
Fragments arranged by size:
3170 569 447 370
EcoRI G'AATT_C
Cuts at: 1560 1560
Size: 4556
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2575 2575
Size: 4556
FspI TGC'GCA
Cuts at: 2337 2337
Size: 4556
HaeII r_GCGC'y
Cuts at: 245 1990 2238 3468 4239 245
Size: 1745 248 1230 771 562
Fragments arranged by size:
1745 1230 771 562 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 383 415 490 522 770 802 1051 1083 2398
Size: 32 75 32 248 32 249 32 1315
Cuts at: 2398 2428 2430 2460 383
Size: 30 2 30 2479
Fragments arranged by size:
2479 1315 249 248 75 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1595 1595
Size: 4556
KasI G'GCGC_C
Cuts at: 1986 2234 1986
Size: 248 4308
KpnI G_GTAC'C
Cuts at: 1576 1576
Size: 4556
MluI A'CGCG_T
Cuts at: 3989 3989
Size: 4556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 322 414 3315 3499 322
Size: 92 2901 184 1379
Fragments arranged by size:
2901 1379 184 92
MscI TGG'CCA
Cuts at: 2317 2317
Size: 4556
MslI CAynn'nnrTG
Cuts at: 655 1294 2545 2672 655
Size: 639 1251 127 2539
Fragments arranged by size:
2539 1251 639 127
MspA1I CmG'CkG
Cuts at: 188 291 938 1446 1667 1981 2341 3125 3370
Size: 103 647 508 221 314 360 784 245
Cuts at: 3370 188
Size: 1374
Fragments arranged by size:
1374 784 647 508 360 314 245 221
103
NaeI GCC'GGC
Cuts at: 2737 2737
Size: 4556
NarI GG'CG_CC
Cuts at: 1987 2235 1987
Size: 248 4308
NgoMIV G'CCGG_C
Cuts at: 2735 2735
Size: 4556
NsiI A_TGCA'T
Cuts at: 843 843
Size: 4556
NspI r_CATG'y
Cuts at: 234 1609 2109 3712 234
Size: 1375 500 1603 1078
Fragments arranged by size:
1603 1375 1078 500
PasI CC'CwG_GG
Cuts at: 1040 1040
Size: 4556
PciI A'CATG_T
Cuts at: 3708 3708
Size: 4556
PflMI CCAn_nnn'nTGG
Cuts at: 1221 1221
Size: 4556
PfoI T'CCnGG_A
Cuts at: 1055 1055
Size: 4556
PmeI GTTT'AAAC
Cuts at: 1555 1555
Size: 4556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3002 3034 3002
Size: 32 4524
PpuMI rG'GwC_Cy
Cuts at: 309 1325 309
Size: 1016 3540
PspXI vC'TCGA_Gb
Cuts at: 1292 1292
Size: 4556
PstI C_TGCA'G
Cuts at: 308 1603 2288 308
Size: 1295 685 2576
Fragments arranged by size:
2576 1295 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4556
PvuII CAG'CTG
Cuts at: 938 1981 2341 938
Size: 1043 360 3153
Fragments arranged by size:
3153 1043 360
RsrII CG'GwC_CG
Cuts at: 2751 2751
Size: 4556
SacI G_AGCT'C
Cuts at: 1570 1570
Size: 4556
SacII CC_GC'GG
Cuts at: 1447 1447
Size: 4556
SalI G'TCGA_C
Cuts at: 1593 1593
Size: 4556
SapI GCTCTTCn'nnn_
Cuts at: 2579 2789 2579
Size: 210 4346
SbfI CC_TGCA'GG
Cuts at: 1603 1603
Size: 4556
ScaI AGT'ACT
Cuts at: 4073 4073
Size: 4556
SfcI C'TryA_G
Cuts at: 33 304 558 1082 1599 2284 3252 3443 33
Size: 271 254 524 517 685 968 191 1146
Fragments arranged by size:
1146 968 685 524 517 271 254 191
SfoI GGC'GCC
Cuts at: 1988 2236 1988
Size: 248 4308
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4556
SmaI CCC'GGG
Cuts at: 1578 1578
Size: 4556
SmlI C'TyrA_G
Cuts at: 375 774 799 1292 1711 2626 3340 3602 375
Size: 399 25 493 419 915 714 262 1329
Fragments arranged by size:
1329 915 714 493 419 399 262 25
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4556
SphI G_CATG'C
Cuts at: 1609 2109 1609
Size: 500 4056
StuI AGG'CCT
Cuts at: 890 1354 890
Size: 464 4092
StyI C'CwwG_G
Cuts at: 530 980 1690 2044 530
Size: 450 710 354 3042
Fragments arranged by size:
3042 710 450 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 494 1260 2139 2272 2731 2940 494
Size: 766 879 133 459 209 2110
Fragments arranged by size:
2110 879 766 459 209 133
TatI w'GTAC_w
Cuts at: 4071 4071
Size: 4556
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4073 4105 4073
Size: 32 4524
Tth111I GACn'n_nGTC
Cuts at: 395 517 1252 2353 395
Size: 122 735 1101 2598
Fragments arranged by size:
2598 1101 735 122
XbaI T'CTAG_A
Cuts at: 1587 1587
Size: 4556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2051 2051
Size: 4556
XhoI C'TCGA_G
Cuts at: 774 1292 774
Size: 518 4038
XmaI C'CCGG_G
Cuts at: 1576 1576
Size: 4556
XmnI GAAnn'nnTTC
Cuts at: 485 1474 485
Size: 989 3567
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AgeI AlfI AloI ApaI AscI AvrII
BaeI BbsI BglI BmtI BsaXI BsiWI BspEI BsrGI
BstXI BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI PacI
PmlI PshAI PsiI PspOMI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI ZraI