Restriction map for
pF1KB8710
(Circular) (Six-base) MAPSORT of: pF1KB8710.seq Check: 4405 from: 1 to: 4313
pF1KB8710 4313 bp
With 182 enzymes: SgfI *
February 20, 2009 16:37 ..
AccI GT'mk_AC
Cuts at: 1351 1351
Size: 4313
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2138 2570 2923 3585 2138
Size: 432 353 662 2866
Fragments arranged by size:
2866 662 432 353
AfeI AGC'GCT
Cuts at: 532 532
Size: 4313
AflIII A'CryG_T
Cuts at: 1021 1665 3465 3746 1021
Size: 644 1800 281 1588
Fragments arranged by size:
1800 1588 644 281
AlwNI CAG_nnn'CTG
Cuts at: 1618 3056 1618
Size: 1438 2875
ApaI G_GGCC'C
Cuts at: 198 198
Size: 4313
ApaLI G'TGCA_C
Cuts at: 3151 3151
Size: 4313
ApoI r'AATT_y
Cuts at: 617 641 930 956 1134 1317 3666 3922 617
Size: 24 289 26 178 183 2349 256 1008
Fragments arranged by size:
2349 1008 289 256 183 178 26 24
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 190 197 211 367 403 1333 190
Size: 7 14 156 36 930 3170
Fragments arranged by size:
3170 930 156 36 14 7
AvrII C'CTAG_G
Cuts at: 1056 1056
Size: 4313
BamHI G'GATC_C
Cuts at: 1122 1338 1122
Size: 216 4097
BanI G'GyrC_C
Cuts at: 351 670 1329 1743 1991 2026 351
Size: 319 659 414 248 35 2638
Fragments arranged by size:
2638 659 414 319 248 35
BanII G_rGCy'C
Cuts at: 144 198 210 410 1327 2357 144
Size: 54 12 200 917 1030 2100
Fragments arranged by size:
2100 1030 917 200 54 12
BbeI G_GCGC'C
Cuts at: 355 1747 1995 355
Size: 1392 248 2673
Fragments arranged by size:
2673 1392 248
BciVI GTATCCnnnnn_n'
Cuts at: 2211 3267 4313 2211
Size: 1056 1046 2211
Fragments arranged by size:
2211 1056 1046
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BfrBI ATG'CAT
Cuts at: 992 992
Size: 4313
BglI GCCn_nnn'nGGC
Cuts at: 299 338 445 299
Size: 39 107 4167
Fragments arranged by size:
4167 107 39
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 1425 1425
Size: 4313
Bme1580I G_kGCm'C
Cuts at: 191 198 404 1938 2031 3155 191
Size: 7 206 1534 93 1124 1349
Fragments arranged by size:
1534 1349 1124 206 93 7
BmrI ACTGGGnnnn_n'
Cuts at: 1525 1606 1941 4085 1525
Size: 81 335 2144 1753
Fragments arranged by size:
2144 1753 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 237 1301 2680 237
Size: 1064 1379 1870
Fragments arranged by size:
1870 1379 1064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 1085
Size: 4313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1489 2368 3082 3380 1489
Size: 879 714 298 2422
Fragments arranged by size:
2422 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 356 4017 356
Size: 3661 652
BsaAI yAC'GTr
Cuts at: 246 1666 246
Size: 1420 2893
BsaBI GATnn'nnATC
Cuts at: 6 745 1827 3864 6
Size: 739 1082 2037 455
Fragments arranged by size:
2037 1082 739 455
BsaHI Gr'CG_yC
Cuts at: 341 352 1744 1992 2694 4190 341
Size: 11 1392 248 702 1496 464
Fragments arranged by size:
1496 1392 702 464 248 11
BsaWI w'CCGG_w
Cuts at: 1502 2023 2701 3112 3259 3533 1502
Size: 521 678 411 147 274 2282
Fragments arranged by size:
2282 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 541 1100 541
Size: 559 3754
BseYI C'CCAG_C
Cuts at: 1225 1738 2496 3161 3569 1225
Size: 513 758 665 408 1969
Fragments arranged by size:
1969 758 665 513 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 444 935 444
Size: 491 3822
BsiEI CG_ry'CG
Cuts at: 104 227 417 509 1855 1901 3131 104
Size: 123 190 92 1346 46 1230 1286
Fragments arranged by size:
1346 1286 1230 190 123 92 46
BsiHKAI G_wGCw'C
Cuts at: 1327 2105 2295 3155 1327
Size: 778 190 860 2485
Fragments arranged by size:
2485 860 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 285 285
Size: 4313
Bsp1286I G_dGCh'C
Cuts at: 144 191 198 210 404 410 1327 1938 2031
Size: 47 7 12 194 6 917 611 93
Cuts at: 2031 2105 2295 2357 3155 144
Size: 74 190 62 798 1302
Fragments arranged by size:
1302 917 798 611 194 190 93 74
62 47 12 7 6
BspHI T'CATG_A
Cuts at: 2745 2745
Size: 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1363 1577 1879 2260 1363
Size: 214 302 381 3416
Fragments arranged by size:
3416 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2605 2659 4143 4308 50
Size: 2555 54 1484 165 55
Fragments arranged by size:
2555 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 722 1258 2225 722
Size: 536 967 2810
Fragments arranged by size:
2810 967 536
BsrFI r'CCGG_y
Cuts at: 1270 2311 2492 1270
Size: 1041 181 3091
Fragments arranged by size:
3091 1041 181
BsrGI T'GTAC_A
Cuts at: 681 1083 681
Size: 402 3911
BssHII G'CGCG_C
Cuts at: 323 377 323
Size: 54 4259
BssSI C'ACGA_G
Cuts at: 449 2584 3292 449
Size: 2135 708 1470
Fragments arranged by size:
2135 1470 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1690 1690
Size: 4313
BstBI TT'CG_AA
Cuts at: 2674 2674
Size: 4313
BstYI r'GATC_y
Cuts at: 1 1122 1338 1805 1828 2163 2409 2715 2727
Size: 1121 216 467 23 335 246 306 12
Cuts at: 2727 2813 3865 1
Size: 86 1052 449
Fragments arranged by size:
1121 1052 467 449 335 306 246 216
86 23 12
BtgI C'CryG_G
Cuts at: 107 227 346 1000 107
Size: 120 119 654 3420
Fragments arranged by size:
3420 654 120 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2263 2444 2607 2263
Size: 181 163 3969
Fragments arranged by size:
3969 181 163
BtsI GCAGTG_nn'
Cuts at: 923 1556 923
Size: 633 3680
DraI TTT'AAA
Cuts at: 1233 1312 1233
Size: 79 4234
DraIII CAC_nnn'GTG
Cuts at: 1219 1219
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 2019 3363 2019
Size: 1344 2969
EaeI y'GGCC_r
Cuts at: 224 291 414 506 719 849 1898 2072 2463
Size: 67 123 92 213 130 1049 174 391
Cuts at: 2463 2490 3835 224
Size: 27 1345 702
Fragments arranged by size:
1345 1049 702 391 213 174 130 123
92 67 27
EagI C'GGCC_G
Cuts at: 224 414 506 1898 224
Size: 190 92 1392 2639
Fragments arranged by size:
2639 1392 190 92
EarI CTCTTCn'nnn_
Cuts at: 606 2336 2546 606
Size: 1730 210 2373
Fragments arranged by size:
2373 1730 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 374 2521 3263 3409 4123 374
Size: 2147 742 146 714 564
Fragments arranged by size:
2147 742 714 564 146
EcoICRI GAG'CTC
Cuts at: 1325 1325
Size: 4313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1085 2138 2570 2923 3585 1085
Size: 1053 432 353 662 1813
Fragments arranged by size:
1813 1053 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 194 1297 1452 194
Size: 1103 155 3055
Fragments arranged by size:
3055 1103 155
EcoRI G'AATT_C
Cuts at: 641 956 1317 641
Size: 315 361 3637
Fragments arranged by size:
3637 361 315
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 355 534 1747 1995 3225 3996 355
Size: 179 1213 248 1230 771 672
Fragments arranged by size:
1230 1213 771 672 248 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 585 617 798 830 1083 1115 1286 1318 2155
Size: 32 181 32 253 32 171 32 837
Cuts at: 2155 2185 2187 2217 585
Size: 30 2 30 2681
Fragments arranged by size:
2681 837 253 181 171 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1352 1352
Size: 4313
KasI G'GCGC_C
Cuts at: 351 1743 1991 351
Size: 1392 248 2673
Fragments arranged by size:
2673 1392 248
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3072 3256 3072
Size: 184 4129
MscI TGG'CCA
Cuts at: 721 851 2074 721
Size: 130 1223 2960
Fragments arranged by size:
2960 1223 130
MslI CAynn'nnrTG
Cuts at: 736 739 2302 2429 736
Size: 3 1563 127 2620
Fragments arranged by size:
2620 1563 127 3
MspA1I CmG'CkG
Cuts at: 698 1129 1424 1738 2098 2882 3127 698
Size: 431 295 314 360 784 245 1884
Fragments arranged by size:
1884 784 431 360 314 295 245
NaeI GCC'GGC
Cuts at: 1272 2494 1272
Size: 1222 3091
NarI GG'CG_CC
Cuts at: 352 1744 1992 352
Size: 1392 248 2673
Fragments arranged by size:
2673 1392 248
NcoI C'CATG_G
Cuts at: 107 1000 107
Size: 893 3420
NgoMIV G'CCGG_C
Cuts at: 1270 2492 1270
Size: 1222 3091
NsiI A_TGCA'T
Cuts at: 994 994
Size: 4313
NspI r_CATG'y
Cuts at: 682 1025 1366 1866 3469 682
Size: 343 341 500 1603 1526
Fragments arranged by size:
1603 1526 500 343 341
PasI CC'CwG_GG
Cuts at: 431 431
Size: 4313
PciI A'CATG_T
Cuts at: 1021 3465 1021
Size: 2444 1869
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4313
PfoI T'CCnGG_A
Cuts at: 1113 1113
Size: 4313
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2759 2791 2759
Size: 32 4281
PpuMI rG'GwC_Cy
Cuts at: 1297 1297
Size: 4313
PspOMI G'GGCC_C
Cuts at: 194 194
Size: 4313
PspXI vC'TCGA_Gb
Cuts at: 367 367
Size: 4313
PstI C_TGCA'G
Cuts at: 1360 2045 1360
Size: 685 3628
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 1129 1738 2098 1129
Size: 609 360 3344
Fragments arranged by size:
3344 609 360
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 1327 1327
Size: 4313
SalI G'TCGA_C
Cuts at: 1350 1350
Size: 4313
SapI GCTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SfcI C'TryA_G
Cuts at: 33 1356 2041 3009 3200 33
Size: 1323 685 968 191 1146
Fragments arranged by size:
1323 1146 968 685 191
SfoI GGC'GCC
Cuts at: 353 1745 1993 353
Size: 1392 248 2673
Fragments arranged by size:
2673 1392 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SmaI CCC'GGG
Cuts at: 192 199 1335 192
Size: 7 1136 3170
Fragments arranged by size:
3170 1136 7
SmlI C'TyrA_G
Cuts at: 211 367 1468 2383 3097 3359 211
Size: 156 1101 915 714 262 1165
Fragments arranged by size:
1165 1101 915 714 262 156
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
SspI AAT'ATT
Cuts at: 767 767
Size: 4313
StyI C'CwwG_G
Cuts at: 107 314 1000 1056 1447 1801 107
Size: 207 686 56 391 354 2619
Fragments arranged by size:
2619 686 391 354 207 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1896 2029 2488 2697 1896
Size: 133 459 209 3512
Fragments arranged by size:
3512 459 209 133
TatI w'GTAC_w
Cuts at: 681 1083 3828 681
Size: 402 2745 1166
Fragments arranged by size:
2745 1166 402
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 566 598 3830 3862 566
Size: 32 3232 32 1017
Fragments arranged by size:
3232 1017 32 32
Tth111I GACn'n_nGTC
Cuts at: 2110 2110
Size: 4313
XbaI T'CTAG_A
Cuts at: 1344 1344
Size: 4313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1808 1808
Size: 4313
XhoI C'TCGA_G
Cuts at: 211 367 211
Size: 156 4157
XmaI C'CCGG_G
Cuts at: 190 197 1333 190
Size: 7 1136 3170
Fragments arranged by size:
3170 1136 7
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AlfI AloI
AscI BaeI BbsI BbvCI BcgI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BspEI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PmlI PshAI
PsiI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XmnI ZraI