Restriction map for pF1KB8710
 (Linear) (Six-base) MAPSORT of: KIBB8710.seq  Check: 1322  from: 1  to: 1200



KIBB8710.orf

 With 182 enzymes: SgfI * 

                        February 20, 2009 16:37  ..

AfeI AGC'GCT

Cuts at:      0     424    1200 
   Size:        424     776
 
AflIII A'CryG_T

Cuts at:      0     913    1200 
   Size:        913     287
 
ApaI G_GGCC'C

Cuts at:      0      90    1200 
   Size:         90    1110
 
ApoI r'AATT_y

Cuts at:      0     509     533     822     848    1026    1200 
   Size:        509      24     289      26     178     174

  Fragments arranged by size: 

                 509     289     178     174      26      24
 
AvaI C'yCGr_G

Cuts at:      0      82      89     103     259     295    1200 
   Size:         82       7      14     156      36     905

  Fragments arranged by size: 

                 905     156      82      36      14       7
 
AvrII C'CTAG_G

Cuts at:      0     948    1200 
   Size:        948     252
 
BamHI G'GATC_C

Cuts at:      0    1014    1200 
   Size:       1014     186
 
BanI G'GyrC_C

Cuts at:      0     243     562    1200 
   Size:        243     319     638

  Fragments arranged by size: 

                 638     319     243
 
BanII G_rGCy'C

Cuts at:      0      36      90     102     302    1200 
   Size:         36      54      12     200     898

  Fragments arranged by size: 

                 898     200      54      36      12
 
BbeI G_GCGC'C

Cuts at:      0     247    1200 
   Size:        247     953
 
BfrBI ATG'CAT

Cuts at:      0     884    1200 
   Size:        884     316
 
BglI GCCn_nnn'nGGC

Cuts at:      0     191     230     337    1200 
   Size:        191      39     107     863

  Fragments arranged by size: 

                 863     191     107      39
 
Bme1580I G_kGCm'C

Cuts at:      0      83      90     296    1200 
   Size:         83       7     206     904

  Fragments arranged by size: 

                 904     206      83       7
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     129    1193    1200 
   Size:        129    1064       7

  Fragments arranged by size: 

                1064     129       7
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     977    1200 
   Size:        977     223
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     248    1200 
   Size:        248     952
 
BsaAI yAC'GTr

Cuts at:      0     138    1200 
   Size:        138    1062
 
BsaBI GATnn'nnATC

Cuts at:      0     637    1200 
   Size:        637     563
 
BsaHI Gr'CG_yC

Cuts at:      0     233     244    1200 
   Size:        233      11     956

  Fragments arranged by size: 

                 956     233      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     433     992    1200 
   Size:        433     559     208

  Fragments arranged by size: 

                 559     433     208
 
BseYI C'CCAG_C

Cuts at:      0    1117    1200 
   Size:       1117      83
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     336     827    1200 
   Size:        336     491     373

  Fragments arranged by size: 

                 491     373     336
 
BsiEI CG_ry'CG

Cuts at:      0     119     309     401    1200 
   Size:        119     190      92     799

  Fragments arranged by size: 

                 799     190     119      92
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     177    1200 
   Size:        177    1023
 
Bsp1286I G_dGCh'C

Cuts at:      0      36      83      90     102     296     302    1200 
   Size:         36      47       7      12     194       6     898

  Fragments arranged by size: 

                 898     194      47      36      12       7       6
 
BsrDI GCAATG_nn'

Cuts at:      0     614    1150    1200 
   Size:        614     536      50

  Fragments arranged by size: 

                 614     536      50
 
BsrFI r'CCGG_y

Cuts at:      0    1162    1200 
   Size:       1162      38
 
BsrGI T'GTAC_A

Cuts at:      0     573     975    1200 
   Size:        573     402     225

  Fragments arranged by size: 

                 573     402     225
 
BssHII G'CGCG_C

Cuts at:      0     215     269    1200 
   Size:        215      54     931

  Fragments arranged by size: 

                 931     215      54
 
BssSI C'ACGA_G

Cuts at:      0     341    1200 
   Size:        341     859
 
BstYI r'GATC_y

Cuts at:      0    1014    1200 
   Size:       1014     186
 
BtgI C'CryG_G

Cuts at:      0     119     238     892    1200 
   Size:        119     119     654     308

  Fragments arranged by size: 

                 654     308     119     119
 
BtsI GCAGTG_nn'

Cuts at:      0     815    1200 
   Size:        815     385
 
DraI TTT'AAA

Cuts at:      0    1125    1200 
   Size:       1125      75
 
DraIII CAC_nnn'GTG

Cuts at:      0    1111    1200 
   Size:       1111      89
 
EaeI y'GGCC_r

Cuts at:      0     116     183     306     398     611     741    1200 
   Size:        116      67     123      92     213     130     459

  Fragments arranged by size: 

                 459     213     130     123     116      92      67
 
EagI C'GGCC_G

Cuts at:      0     116     306     398    1200 
   Size:        116     190      92     802

  Fragments arranged by size: 

                 802     190     116      92
 
EarI CTCTTCn'nnn_

Cuts at:      0     498    1200 
   Size:        498     702
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     266    1200 
   Size:        266     934
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     977    1200 
   Size:        977     223
 
EcoO109I rG'GnC_Cy

Cuts at:      0      86    1189    1200 
   Size:         86    1103      11

  Fragments arranged by size: 

                1103      86      11
 
EcoRI G'AATT_C

Cuts at:      0     533     848    1200 
   Size:        533     315     352

  Fragments arranged by size: 

                 533     352     315
 
HaeII r_GCGC'y

Cuts at:      0     247     426    1200 
   Size:        247     179     774

  Fragments arranged by size: 

                 774     247     179
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     477     509     690     722     975    1007    1178    1200 
   Size:        477      32     181      32     253      32     171      22

  Fragments arranged by size: 

                 477     253     181     171      32      32      32      22
            
 
KasI G'GCGC_C

Cuts at:      0     243    1200 
   Size:        243     957
 
MscI TGG'CCA

Cuts at:      0     613     743    1200 
   Size:        613     130     457

  Fragments arranged by size: 

                 613     457     130
 
MslI CAynn'nnrTG

Cuts at:      0     628     631    1200 
   Size:        628       3     569

  Fragments arranged by size: 

                 628     569       3
 
MspA1I CmG'CkG

Cuts at:      0     590    1021    1200 
   Size:        590     431     179

  Fragments arranged by size: 

                 590     431     179
 
NaeI GCC'GGC

Cuts at:      0    1164    1200 
   Size:       1164      36
 
NarI GG'CG_CC

Cuts at:      0     244    1200 
   Size:        244     956
 
NcoI C'CATG_G

Cuts at:      0     892    1200 
   Size:        892     308
 
NgoMIV G'CCGG_C

Cuts at:      0    1162    1200 
   Size:       1162      38
 
NsiI A_TGCA'T

Cuts at:      0     886    1200 
   Size:        886     314
 
NspI r_CATG'y

Cuts at:      0     574     917    1200 
   Size:        574     343     283

  Fragments arranged by size: 

                 574     343     283
 
PasI CC'CwG_GG

Cuts at:      0     323    1200 
   Size:        323     877
 
PciI A'CATG_T

Cuts at:      0     913    1200 
   Size:        913     287
 
PfoI T'CCnGG_A

Cuts at:      0    1005    1200 
   Size:       1005     195
 
PpuMI rG'GwC_Cy

Cuts at:      0    1189    1200 
   Size:       1189      11
 
PspOMI G'GGCC_C

Cuts at:      0      86    1200 
   Size:         86    1114
 
PspXI vC'TCGA_Gb

Cuts at:      0     259    1200 
   Size:        259     941
 
PvuII CAG'CTG

Cuts at:      0    1021    1200 
   Size:       1021     179
 
SfoI GGC'GCC

Cuts at:      0     245    1200 
   Size:        245     955
 
SmaI CCC'GGG

Cuts at:      0      84      91    1200 
   Size:         84       7    1109

  Fragments arranged by size: 

                1109      84       7
 
SmlI C'TyrA_G

Cuts at:      0     103     259    1200 
   Size:        103     156     941

  Fragments arranged by size: 

                 941     156     103
 
SspI AAT'ATT

Cuts at:      0     659    1200 
   Size:        659     541
 
StyI C'CwwG_G

Cuts at:      0     206     892     948    1200 
   Size:        206     686      56     252

  Fragments arranged by size: 

                 686     252     206      56
 
TatI w'GTAC_w

Cuts at:      0     573     975    1200 
   Size:        573     402     225

  Fragments arranged by size: 

                 573     402     225
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     458     490    1200 
   Size:        458      32     710

  Fragments arranged by size: 

                 710     458      32
 
XhoI C'TCGA_G

Cuts at:      0     103     259    1200 
   Size:        103     156     941

  Fragments arranged by size: 

                 941     156     103
 
XmaI C'CCGG_G

Cuts at:      0      82      89    1200 
   Size:         82       7    1111

  Fragments arranged by size: 

                1111      82       7
 

 Enzymes that do cut:

     AfeI   AflIII     ApaI     ApoI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI    BfrBI     BglI Bme1580I     BplI     BpmI     BsaI
    BsaAI    BsaBI    BsaHI    BseRI    BseYI     BsgI    BsiEI    BsmBI
 Bsp1286I    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstYI     BtgI
     BtsI     DraI   DraIII     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI    HaeII    Hin4I     KasI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PasI     PciI
     PfoI    PpuMI   PspOMI    PspXI    PvuII     SfoI     SmaI     SmlI
     SspI     StyI     TatI     TstI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     BaeI     BbsI    BbvCI     BcgI    BciVI     BclI    BglII
     BlpI    BmgBI     BmrI     BmtI   Bpu10I    BpuEI    BsaWI    BsaXI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DrdI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NdeI
     NheI     NotI     NruI     PacI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XmnI     ZraI
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