Restriction map for pF1KB8705
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BciVI       BfrI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspMI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FauI        FokI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HphI        KasI        KpnI        Ksp632I     
KspI        MaeIII      MamI        MfeI        MluI        
MroI        MspA1I      MunI        MvaI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PstI        
PvuI        PvuII       RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SpeI        SphI        Sse8387I    
SspBI       StuI        StyI        SwaI        Tsp45I      
Van91I      XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AciI          307  GCGG                 CCGC(-3/-1)
AcsI          777  AAATTT               [GA]^AATT[CT]
AflIII        209  ACATGT               A^C[GA][CT]GT
AlwI          799  GATCC                GGATC(4/5)
AlwNI         248  CAGCTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          777  AAATTT               [GA]^AATT[CT]
AspHI         542  GTGCTC               G[AT]GC[AT]^C
AspI          518  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           66  CTCGGG               C^[CT]CG[GA]G
BcgI          219  CGATAGCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          618  TGATCA               T^GATCA
BcnI          104  CCCGG                CC^[GC]GG
BglI          229  GCCATTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          62  AGATCT               A^GATCT
BmyI          542  GTGCTC               G[AGT]GC[ACT]^C
BsaAI        1102  TACGTA               [CT]AC^GT[GA]
BsaJI         513  CCACGG               C^C[ACGT][ACGT]GG
BseRI         890  CTCCTC               GAGGAG(10/8)
BsgI          605  CTGCAC               GTGCAG(16/14)
BsiHKAI       542  GTGCTC               G[AT]GC[AT]^C
BsiYI         434  CCATTCTTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          434  CCATTCTTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI        1006  GGGAC                GGGAC(10/14)
BsoBI          66  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      542  GTGCTC               G[AGT]GC[ACT]^C
BspLU11I      209  ACATGT               A^CATGT
BsrBI         305  GAGCGG               CCGCTC(-3/-3)
BsrDI         574  CATTGC               GCAATG(2/0)
BsrI          243  ACTGG                ACTGG(1/-1)
BssKI         104  CCCGG                ^CC[ACGT]GG
BstAPI       1027  GCACCTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I         319  GCTGGC               GC[ACGT]^[ACGT]GC
DsaI          513  CCACGG               C^C[GA][CT]GG
FbaI          618  TGATCA               T^GATCA
HapII         105  CCGG                 C^CGG
HpaII         105  CCGG                 C^CGG
MspI          105  CCGG                 C^CGG
NciI          104  CCCGG                CC^[GC]GG
NspI          209  ACATGT               [GA]CATG^[CT]
PflFI         518  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          199  GACTC                GAGTC(4/5)
ScrFI         104  CCCGG                CC^[ACGT]GG
SnaBI        1102  TACGTA               TAC^GTA
TfiI          862  GAATC                G^A[AT]TC
Tth111I       518  GACACAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          546  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          934  GTAC                 GT^AC
             1105  GTAC                
AluI          249  AGCT                 AG^CT
             1097  AGCT                
AseI          876  ATTAAT               AT^TAAT
             1017  ATTAAT              
AsnI          876  ATTAAT               AT^TAAT
             1017  ATTAAT              
AvaII         682  GGACC                G^G[AT]CC
             1007  GGACC               
BalI          510  TGGCCA               TGG^CCA
              561  TGGCCA              
BsmAI         417  GAGAC                GTCTC(1/5)
             1092  GAGAC               
BspHI         651  TCATGA               T^CATGA
              767  TCATGA              
BstYI          62  AGATCT               [GA]^GATC[CT]
              798  AGATCC              
DraII         681  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1006  GGGACCT             
EaeI          510  TGGCCA               [CT]^GGCC[GA]
              561  TGGCCA              
EcoO109I      681  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1006  GGGACCT             
HinfI         199  GACTC                G^A[ACGT]TC
              862  GAATC               
MaeII         995  ACGT                 A^CGT
             1103  ACGT                
MboII         119  TCTTC                GAAGA(8/7)
              347  TCTTC               
MflI           62  AGATCT               [GA]^GATC[CT]
              798  AGATCC              
MluNI         510  TGGCCA               TGG^CCA
              561  TGGCCA              
MscI          510  TGGCCA               TGG^CCA
              561  TGGCCA              
PpuMI         681  AGGACCC              [GA]G^G[AT]CC[CT]
             1006  GGGACCT             
PshBI         876  ATTAAT               AT^TAAT
             1017  ATTAAT              
RcaI          651  TCATGA               T^CATGA
              767  TCATGA              
RsaI          934  GTAC                 GT^AC
             1105  GTAC                
TaqI          218  TCGA                 T^CGA
              569  TCGA                
TthHB8I       218  TCGA                 T^CGA
              569  TCGA                
XhoII          62  AGATCT               [GA]^GATC[CT]
              798  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

BbvI        BfaI        BpmI        Cfr13I      DdeI        
DpnII       Fnu4HI      HaeIII      ItaI        MaeI        
MboI        MnlI        MseI        MslI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
SfaNI       SspI        Tru9I       TseI        Tsp509I     
TspRI       
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