Restriction map for pF1KB8703
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BciVI       BclI        BfrI        BlnI        BlpI        
Bpu1102I    BsaBI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BsrBI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MseI        MslI        MunI        MvnI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TfiI        Tru9I       XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII        398  TCCGGA               T^CCGGA
AcsI          967  GAATTC               [GA]^AATT[CT]
AflIII       1223  ACATGT               A^C[GA][CT]GT
ApaI          652  GGGCCC               GGGCC^C
ApoI          967  GAATTC               [GA]^AATT[CT]
Asp700I       967  GAATTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         602  GTGCTC               G[AT]GC[AT]^C
BalI         1568  TGGCCA               TGG^CCA
BbeI          455  GGCGCC               GGCGC^C
BbrPI        1156  CACGTG               CAC^GTG
BbsI           92  GTCTTC               GAAGAC(2/6)
BcgI          355  CGAAAAACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          598  CTAG                 C^TAG
BglII        1685  AGATCT               A^GATCT
BpuAI          92  GTCTTC               GAAGAC(2/6)
BsaAI        1156  CACGTG               [CT]AC^GT[GA]
BsaWI         398  TCCGGA               [AT]^CCGG[AT]
BseAI         398  TCCGGA               T^CCGGA
BsgI          819  GTGCAG               GTGCAG(16/14)
BsiHKAI       602  GTGCTC               G[AT]GC[AT]^C
BspEI         398  TCCGGA               T^CCGGA
BspLU11I     1223  ACATGT               A^CATGT
DrdI          684  GACTCCAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         967  GAATTC               G^AATTC
HindIII       445  AAGCTT               A^AGCTT
KasI          455  GGCGCC               G^GCGCC
MaeI          598  CTAG                 C^TAG
MluNI        1568  TGGCCA               TGG^CCA
MroI          398  TCCGGA               T^CCGGA
MscI         1568  TGGCCA               TGG^CCA
NarI          455  GGCGCC               GG^CGCC
PflMI         148  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1156  CACGTG               CAC^GTG
PmlI         1156  CACGTG               CAC^GTG
PshAI         122  GACTCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI         1613  AGTACT               AGT^ACT
SfcI         1495  CTACAG               C^T[GA][CT]AG
SfoI          455  GGCGCC               GGC^GCC
SmaI          566  CCCGGG               CCC^GGG
StuI          892  AGGCCT               AGG^CCT
TaqI          354  TCGA                 T^CGA
Tsp509I       968  AATT                 ^AATT
TthHB8I       354  TCGA                 T^CGA
Van91I        148  CCAGATCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         566  CCCGGG               C^CCGGG
XmaI          566  CCCGGG               C^CCGGG
XmnI          967  GAATTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AlwI          151  GATCC                GGATC(4/5)
             1089  GATCC               
AvaI          566  CCCGGG               C^[CT]CG[GA]G
              711  CTCGGG              
BanII         652  GGGCCC               G[GA]GC[CT]^C
              858  GGGCTC              
BglI          458  GCCACCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              645  GCCCTCTGGGC         
BpmI         1027  CTGGAG               CTGGAG(16/14)
             1384  CTGGAG              
BsaI         1232  GAGACC               GGTCTC(1/5)
              632  GGTCTC              
BsoBI         566  CCCGGG               C^[CT]CG[GA]G
              711  CTCGGG              
BspMI         361  ACCTGC               ACCTGC(4/8)
              943  ACCTGC              
BsrDI         812  GCAATG               GCAATG(2/0)
             1115  GCAATG              
BsrFI         467  GCCGGC               [GA]^CCGG[CT]
              907  GCCGGC              
BstAPI        812  GCAATGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1659  GCAGGACATGC         
BstXI        1334  CCAAAAACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1376  CCATCCACCTGG        
BstYI         150  AGATCC               [GA]^GATC[CT]
             1685  AGATCT              
Cfr10I        467  GCCGGC               [GA]^CCGG[CT]
              907  GCCGGC              
EaeI          909  CGGCCA               [CT]^GGCC[GA]
             1568  TGGCCA              
EcoNI         154  CCTGGGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              924  CCTGCGAGAGG         
HaeII         371  AGCGCC               [GA]GCGC^[CT]
              455  GGCGCC              
MflI          150  AGATCC               [GA]^GATC[CT]
             1685  AGATCT              
NaeI          467  GCCGGC               GCC^GGC
              907  GCCGGC              
NgoMIV        467  GCCGGC               G^CCGGC
              907  GCCGGC              
PpuMI          25  GGGTCCT              [GA]G^G[AT]CC[CT]
             1180  GGGACCT             
PvuII         310  CAGCTG               CAG^CTG
             1084  CAGCTG              
SexAI        1211  ACCAGGT              A^CC[AT]GGT
             1235  ACCTGGT             
SmlI           72  CTCAAG               C^T[CT][GA]AG
              124  CTCAAG              
XcmI         1064  CCAGGAAGGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1166  CCATGGATGACTTGG     
XhoII         150  AGATCC               [GA]^GATC[CT]
             1685  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwNI       
AspI        AvaII       BanI        BbvCI       BbvI        
BcnI        BmyI        BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DsaI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflFI       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TseI        Tsp45I      TspRI       
Tth111I     
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