Restriction map for
pF1KB8695
(Circular) (Six-base) MAPSORT of: pF1KB8695.seq Check: 3032 from: 1 to: 5273
pF1KB8695 5273 bp
With 182 enzymes: SgfI *
June 20, 2008 10:29 ..
AccI GT'mk_AC
Cuts at: 243 2311 243
Size: 2068 3205
Acc65I G'GTAC_C
Cuts at: 2289 2289
Size: 5273
AclI AA'CG_TT
Cuts at: 5113 5113
Size: 5273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 1310 1351 3098 3530 3883 4545 1135
Size: 175 41 1747 432 353 662 1863
Fragments arranged by size:
1863 1747 662 432 353 175 41
AflII C'TTAA_G
Cuts at: 1729 1729
Size: 5273
AflIII A'CryG_T
Cuts at: 1780 2625 4425 4706 1780
Size: 845 1800 281 2347
Fragments arranged by size:
2347 1800 845 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 321 321
Size: 5273
AlwNI CAG_nnn'CTG
Cuts at: 859 892 2578 4016 859
Size: 33 1686 1438 2116
Fragments arranged by size:
2116 1686 1438 33
ApaLI G'TGCA_C
Cuts at: 4111 4111
Size: 5273
ApoI r'AATT_y
Cuts at: 509 703 1282 1385 1436 2277 4626 4882 509
Size: 194 579 103 51 841 2349 256 900
Fragments arranged by size:
2349 900 841 579 256 194 103 51
AseI AT'TA_AT
Cuts at: 20 116 740 2207 20
Size: 96 624 1467 3086
Fragments arranged by size:
3086 1467 624 96
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
AvaI C'yCGr_G
Cuts at: 1196 2293 1196
Size: 1097 4176
BamHI G'GATC_C
Cuts at: 1410 2298 1410
Size: 888 4385
BanI G'GyrC_C
Cuts at: 1561 2289 2703 2951 2986 1561
Size: 728 414 248 35 3848
Fragments arranged by size:
3848 728 414 248 35
BanII G_rGCy'C
Cuts at: 2287 3317 2287
Size: 1030 4243
BbeI G_GCGC'C
Cuts at: 2707 2955 2707
Size: 248 5025
BciVI GTATCCnnnnn_n'
Cuts at: 1899 3171 4227 5273 1899
Size: 1272 1056 1046 1899
Fragments arranged by size:
1899 1272 1056 1046
BclI T'GATC_A
Cuts at: 1098 2793 1098
Size: 1695 3578
BfrBI ATG'CAT
Cuts at: 1696 1842 1696
Size: 146 5127
BglII A'GATC_T
Cuts at: 847 2788 4825 847
Size: 1941 2037 1295
Fragments arranged by size:
2037 1941 1295
BlpI GC'TnA_GC
Cuts at: 2385 2385
Size: 5273
Bme1580I G_kGCm'C
Cuts at: 1900 2898 2991 4115 1900
Size: 998 93 1124 3058
Fragments arranged by size:
3058 1124 998 93
BmrI ACTGGGnnnn_n'
Cuts at: 2485 2566 2901 5045 2485
Size: 81 335 2144 2713
Fragments arranged by size:
2713 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3640 3640
Size: 5273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 836 1465 243
Size: 593 629 4051
Fragments arranged by size:
4051 629 593
Bpu10I CC'TnA_GC
Cuts at: 212 212
Size: 5273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2449 3328 4042 4340 2449
Size: 879 714 298 3382
Fragments arranged by size:
3382 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4977 4977
Size: 5273
BsaAI yAC'GTr
Cuts at: 2626 2626
Size: 5273
BsaBI GATnn'nnATC
Cuts at: 6 2787 4824 6
Size: 2781 2037 455
Fragments arranged by size:
2781 2037 455
BsaHI Gr'CG_yC
Cuts at: 2704 2952 3654 5150 2704
Size: 248 702 1496 2827
Fragments arranged by size:
2827 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2462 2983 3661 4072 4219 4493 2462
Size: 521 678 411 147 274 3242
Fragments arranged by size:
3242 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 360 390 1292 1322 360
Size: 30 902 30 4311
Fragments arranged by size:
4311 902 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1144 1144
Size: 5273
BseYI C'CCAG_C
Cuts at: 2698 3456 4121 4529 2698
Size: 758 665 408 3442
Fragments arranged by size:
3442 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1733 1733
Size: 5273
BsiEI CG_ry'CG
Cuts at: 104 2815 2861 4091 104
Size: 2711 46 1230 1286
Fragments arranged by size:
2711 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2287 3065 3255 4115 2287
Size: 778 190 860 3445
Fragments arranged by size:
3445 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1900 2287 2898 2991 3065 3255 3317 4115 1900
Size: 387 611 93 74 190 62 798 3058
Fragments arranged by size:
3058 798 611 387 190 93 74 62
BspHI T'CATG_A
Cuts at: 3705 3705
Size: 5273
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2323 2537 2839 3220 2323
Size: 214 302 381 4376
Fragments arranged by size:
4376 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3565 3619 5103 5268 50
Size: 3515 54 1484 165 55
Fragments arranged by size:
3515 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3185 3185
Size: 5273
BsrFI r'CCGG_y
Cuts at: 3271 3452 3271
Size: 181 5092
BsrGI T'GTAC_A
Cuts at: 894 999 894
Size: 105 5168
BssSI C'ACGA_G
Cuts at: 3544 4252 3544
Size: 708 4565
BstAPI GCAn_nnn'nTGC
Cuts at: 2650 2650
Size: 5273
BstBI TT'CG_AA
Cuts at: 3634 3634
Size: 5273
BstYI r'GATC_y
Cuts at: 1 847 855 1410 1744 2298 2765 2788 3123
Size: 846 8 555 334 554 467 23 335
Cuts at: 3123 3369 3675 3687 3773 4825 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 846 555 554 467 449 335 334
306 246 86 23 12 8
Bsu36I CC'TnA_GG
Cuts at: 1787 1787
Size: 5273
BtgI C'CryG_G
Cuts at: 336 989 336
Size: 653 4620
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3223 3404 3567 3223
Size: 181 163 4929
Fragments arranged by size:
4929 181 163
BtsI GCAGTG_nn'
Cuts at: 2516 2516
Size: 5273
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 687 722 1451 1486 687
Size: 35 729 35 4474
Fragments arranged by size:
4474 729 35 35
DraI TTT'AAA
Cuts at: 2272 2272
Size: 5273
DrdI GACnn_nn'nnGTC
Cuts at: 2979 4323 2979
Size: 1344 3929
EaeI y'GGCC_r
Cuts at: 1861 2858 3032 3423 3450 4795 1861
Size: 997 174 391 27 1345 2339
Fragments arranged by size:
2339 1345 997 391 174 27
EagI C'GGCC_G
Cuts at: 2858 2858
Size: 5273
EarI CTCTTCn'nnn_
Cuts at: 617 827 1076 1154 1370 3296 3506 617
Size: 210 249 78 216 1926 210 2384
Fragments arranged by size:
2384 1926 249 216 210 210 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3481 4223 4369 5083 3481
Size: 742 146 714 3671
Fragments arranged by size:
3671 742 714 146
EcoICRI GAG'CTC
Cuts at: 2285 2285
Size: 5273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 243 836 1135 1310 1351 1465 3098 3530 3883
Size: 593 299 175 41 114 1633 432 353
Cuts at: 3883 4545 243
Size: 662 971
Fragments arranged by size:
1633 971 662 593 432 353 299 175
114 41
EcoO109I rG'GnC_Cy
Cuts at: 1193 2412 1193
Size: 1219 4054
EcoRI G'AATT_C
Cuts at: 1436 2277 1436
Size: 841 4432
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3292 3292
Size: 5273
FspI TGC'GCA
Cuts at: 3054 3054
Size: 5273
HaeII r_GCGC'y
Cuts at: 2707 2955 4185 4956 2707
Size: 248 1230 771 3024
Fragments arranged by size:
3024 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1257 1289 1292 1324 3115 3145 3147 3177 1257
Size: 32 3 32 1791 30 2 30 3353
Fragments arranged by size:
3353 1791 32 32 30 30 3 2
HincII GTy'rAC
Cuts at: 2312 2312
Size: 5273
HindIII A'AGCT_T
Cuts at: 1004 1944 1004
Size: 940 4333
KasI G'GCGC_C
Cuts at: 2703 2951 2703
Size: 248 5025
KpnI G_GTAC'C
Cuts at: 2293 2293
Size: 5273
MfeI C'AATT_G
Cuts at: 1445 1445
Size: 5273
MluI A'CGCG_T
Cuts at: 4706 4706
Size: 5273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1647 1666 2096 4032 4216 1647
Size: 19 430 1936 184 2704
Fragments arranged by size:
2704 1936 430 184 19
MscI TGG'CCA
Cuts at: 1863 3034 1863
Size: 1171 4102
MslI CAynn'nnrTG
Cuts at: 406 3262 3389 406
Size: 2856 127 2290
Fragments arranged by size:
2856 2290 127
MspA1I CmG'CkG
Cuts at: 2109 2384 2698 3058 3842 4087 2109
Size: 275 314 360 784 245 3295
Fragments arranged by size:
3295 784 360 314 275 245
NaeI GCC'GGC
Cuts at: 3454 3454
Size: 5273
NarI GG'CG_CC
Cuts at: 2704 2952 2704
Size: 248 5025
NcoI C'CATG_G
Cuts at: 336 336
Size: 5273
NdeI CA'TA_TG
Cuts at: 1595 1595
Size: 5273
NgoMIV G'CCGG_C
Cuts at: 3452 3452
Size: 5273
NsiI A_TGCA'T
Cuts at: 1698 1844 1698
Size: 146 5127
NspI r_CATG'y
Cuts at: 1784 2326 2826 4429 1784
Size: 542 500 1603 2628
Fragments arranged by size:
2628 1603 542 500
PasI CC'CwG_GG
Cuts at: 1893 1893
Size: 5273
PciI A'CATG_T
Cuts at: 1780 4425 1780
Size: 2645 2628
PflMI CCAn_nnn'nTGG
Cuts at: 859 859
Size: 5273
PfoI T'CCnGG_A
Cuts at: 797 797
Size: 5273
PmeI GTTT'AAAC
Cuts at: 2272 2272
Size: 5273
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 3719
Size: 32 5241
PpuMI rG'GwC_Cy
Cuts at: 1193 1193
Size: 5273
PsiI TTA'TAA
Cuts at: 875 875
Size: 5273
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 187 219 187
Size: 32 5241
PstI C_TGCA'G
Cuts at: 1377 2114 2201 2320 3005 1377
Size: 737 87 119 685 3645
Fragments arranged by size:
3645 737 685 119 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5273
PvuII CAG'CTG
Cuts at: 2109 2698 3058 2109
Size: 589 360 4324
Fragments arranged by size:
4324 589 360
RsrII CG'GwC_CG
Cuts at: 3468 3468
Size: 5273
SacI G_AGCT'C
Cuts at: 2287 2287
Size: 5273
SalI G'TCGA_C
Cuts at: 2310 2310
Size: 5273
SapI GCTCTTCn'nnn_
Cuts at: 1076 3296 3506 1076
Size: 2220 210 2843
Fragments arranged by size:
2843 2220 210
SbfI CC_TGCA'GG
Cuts at: 2320 2320
Size: 5273
ScaI AGT'ACT
Cuts at: 426 4790 426
Size: 4364 909
SfcI C'TryA_G
Cuts at: 33 125 1373 1982 2110 2197 2316 3001 3969
Size: 92 1248 609 128 87 119 685 968
Cuts at: 3969 4160 33
Size: 191 1146
Fragments arranged by size:
1248 1146 968 685 609 191 128 119
92 87
SfoI GGC'GCC
Cuts at: 2705 2953 2705
Size: 248 5025
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5273
SmaI CCC'GGG
Cuts at: 1198 2295 1198
Size: 1097 4176
SmlI C'TyrA_G
Cuts at: 1729 2428 3343 4057 4319 1729
Size: 699 915 714 262 2683
Fragments arranged by size:
2683 915 714 699 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5273
SphI G_CATG'C
Cuts at: 2326 2826 2326
Size: 500 4773
SspI AAT'ATT
Cuts at: 2174 2174
Size: 5273
StyI C'CwwG_G
Cuts at: 336 380 1601 2407 2761 336
Size: 44 1221 806 354 2848
Fragments arranged by size:
2848 1221 806 354 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2856 2989 3448 3657 2856
Size: 133 459 209 4472
Fragments arranged by size:
4472 459 209 133
TatI w'GTAC_w
Cuts at: 424 894 999 4788 424
Size: 470 105 3789 909
Fragments arranged by size:
3789 909 470 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 276 308 360 392 1286 1318 4790 4822 276
Size: 32 52 32 894 32 3472 32 727
Fragments arranged by size:
3472 894 727 52 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3070 3070
Size: 5273
XbaI T'CTAG_A
Cuts at: 1278 2076 2304 1278
Size: 798 228 4247
Fragments arranged by size:
4247 798 228
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2768 2768
Size: 5273
XmaI C'CCGG_G
Cuts at: 1196 2293 1196
Size: 1097 4176
XmnI GAAnn'nnTTC
Cuts at: 1364 1724 2246 1364
Size: 360 522 4391
Fragments arranged by size:
4391 522 360
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BglI BmgBI
BmtI BsiWI BsmI BsmBI BspEI BssHII BstEII BstXI
BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI
NheI NotI NruI PacI PmlI PshAI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI