Restriction map for pF1KB8694
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AclI        AfaI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       BalI        
BbrPI       BbsI        BbvCI       BcgI        BclI        
BcnI        BfrI        BglI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraIII      
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        HpaI        
HphI        KpnI        KspI        MamI        MfeI        
MluNI       MscI        MspA1I      MunI        MwoI        
NaeI        NciI        NdeI        NgoMIV      NheI        
NotI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacII       
SapI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
Van91I      XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI          452  GTCGAC               GT^[AC][GT]AC
AccIII       1078  TCCGGA               T^CCGGA
AcsI          742  AAATTC               [GA]^AATT[CT]
AcyI          226  GGCGCC               G[GA]^CG[CT]C
AflIII       1203  ACGCGT               A^C[GA][CT]GT
ApoI          742  AAATTC               [GA]^AATT[CT]
AspHI         497  GAGCTC               G[AT]GC[AT]^C
AvaII         347  GGTCC                G^G[AT]CC
AvrII         906  CCTAGG               C^CTAGG
BamHI         910  GGATCC               G^GATCC
BanII         497  GAGCTC               G[GA]GC[CT]^C
BbeI          226  GGCGCC               GGCGC^C
BciVI         750  GGATAC               GTATCC(6/5)
BglII         320  AGATCT               A^GATCT
BlnI          906  CCTAGG               C^CTAGG
BmyI          497  GAGCTC               G[AGT]GC[ACT]^C
BsaHI         226  GGCGCC               G[GA]^CG[CT]C
BsaI          262  GAGACC               GGTCTC(1/5)
BsaWI        1078  TCCGGA               [AT]^CCGG[AT]
BseAI        1078  TCCGGA               T^CCGGA
BseRI          58  GAGGAG               GAGGAG(10/8)
BsgI          361  GTGCAG               GTGCAG(16/14)
BsiHKAI       497  GAGCTC               G[AT]GC[AT]^C
BsmFI          39  GTCCC                GGGAC(10/14)
Bsp1286I      497  GAGCTC               G[AGT]GC[ACT]^C
BspEI        1078  TCCGGA               T^CCGGA
DdeI          324  CTCAG                C^T[ACGT]AG
DraI           76  TTTAAA               TTT^AAA
DraII         346  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          879  CCATGG               C^C[GA][CT]GG
EcoO109I      346  GGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         187  GATATC               GAT^ATC
FauI          267  CCCGC                CCCGC(4/6)
HapII        1079  CCGG                 C^CGG
Hin1I         226  GGCGCC               G[GA]^CG[CT]C
HindIII       110  AAGCTT               A^AGCTT
HpaII        1079  CCGG                 C^CGG
KasI          226  GGCGCC               G^GCGCC
MaeII         962  ACGT                 A^CGT
MluI         1203  ACGCGT               A^CGCGT
MroI         1078  TCCGGA               T^CCGGA
MslI          991  CATGTAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         1079  CCGG                 C^CGG
NarI          226  GGCGCC               GG^CGCC
NcoI          879  CCATGG               C^CATGG
NruI         1270  TCGCGA               TCG^CGA
PaeR7I        587  CTCGAG               C^TCGAG
PpuMI         346  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          807  CTGCAG               CTGCA^G
SacI          497  GAGCTC               GAGCT^C
SalI          452  GTCGAC               G^TCGAC
SfcI          807  CTGCAG               C^T[GA][CT]AG
SfoI          226  GGCGCC               GGC^GCC
SspI          719  AATATT               AAT^ATT
XcmI          968  CCAGCCGAGGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          587  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AccII        1204  CGCG                 CG^CG
             1271  CGCG                
AlwNI         203  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
              810  CAGCACCTG           
BanI            6  GGCACC               G^G[CT][GA]CC
              226  GGCGCC              
BsiEI          29  CGACCG               CG[GA][CT]^CG
              528  CGACCG              
BsrI          208  ACTGG                ACTGG(1/-1)
              120  CCAGT               
BstUI        1204  CGCG                 CG^CG
             1271  CGCG                
DrdI           30  GACCGAAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              884  GACAAATGAGTC        
HgaI           37  GCGTC                GACGC(5/10)
             1205  GCGTC               
MvnI         1204  CGCG                 CG^CG
             1271  CGCG                
PleI          300  GACTC                GAGTC(4/5)
              891  GAGTC               
SexAI         758  ACCTGGT              A^CC[AT]GGT
             1158  ACCAGGT             
SfaNI        1191  GATGC                GCATC(5/9)
             1279  GATGC               
SmlI          492  CTTGAG               C^T[CT][GA]AG
              587  CTCGAG              
TspRI         254  CACTG                CA[GC]TG[ACGT][ACGT]^
              864  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AluI        AlwI        Asp700I     AvaI        
BbvI        BfaI        BpmI        BsaJI       BsiYI       
BslI        BsmAI       BsoBI       BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DpnII       EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HhaI        HinP1I      
HincII      HindII      HinfI       ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MvaI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TthHB8I     XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com