Restriction map for pF1KB8694
 (Linear) (Six-base) MAPSORT of: KIBB8694.seq  Check: 4675  from: 1  to: 1365



KIBB8694.orf

 With 182 enzymes: SgfI * 

                        July 2, 2008 13:23  ..

AccI GT'mk_AC

Cuts at:      0     453    1365 
   Size:        453     912
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     679    1232    1365 
   Size:        628      51     553     133

  Fragments arranged by size: 

                 628     553     133      51
 
AflIII A'CryG_T

Cuts at:      0    1203    1365 
   Size:       1203     162
 
AlwNI CAG_nnn'CTG

Cuts at:      0     208     815    1365 
   Size:        208     607     550

  Fragments arranged by size: 

                 607     550     208
 
ApoI r'AATT_y

Cuts at:      0     742    1365 
   Size:        742     623
 
AvaI C'yCGr_G

Cuts at:      0      10     302     587    1365 
   Size:         10     292     285     778

  Fragments arranged by size: 

                 778     292     285      10
 
AvrII C'CTAG_G

Cuts at:      0     906    1365 
   Size:        906     459
 
BamHI G'GATC_C

Cuts at:      0     910    1365 
   Size:        910     455
 
BanI G'GyrC_C

Cuts at:      0       6     226    1365 
   Size:          6     220    1139

  Fragments arranged by size: 

                1139     220       6
 
BanII G_rGCy'C

Cuts at:      0     501    1365 
   Size:        501     864
 
BbeI G_GCGC'C

Cuts at:      0     230    1365 
   Size:        230    1135
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     744    1365 
   Size:        744     621
 
BglII A'GATC_T

Cuts at:      0     320    1365 
   Size:        320    1045
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280    1304    1355    1365 
   Size:        280    1024      51      10

  Fragments arranged by size: 

                1024     280      51      10
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     513    1365 
   Size:        513     852
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     256    1365 
   Size:        256    1109
 
BsaHI Gr'CG_yC

Cuts at:      0     227    1365 
   Size:        227    1138
 
BsaWI w'CCGG_w

Cuts at:      0    1078    1365 
   Size:       1078     287
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73    1365 
   Size:         73    1292
 
BseYI C'CCAG_C

Cuts at:      0      41    1069    1365 
   Size:         41    1028     296

  Fragments arranged by size: 

                1028     296      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     382    1365 
   Size:        382     983
 
BsiEI CG_ry'CG

Cuts at:      0      32     531    1365 
   Size:         32     499     834

  Fragments arranged by size: 

                 834     499      32
 
BsiHKAI G_wGCw'C

Cuts at:      0     501    1365 
   Size:        501     864
 
Bsp1286I G_dGCh'C

Cuts at:      0     501    1365 
   Size:        501     864
 
BspEI T'CCGG_A

Cuts at:      0    1078    1365 
   Size:       1078     287
 
BstYI r'GATC_y

Cuts at:      0     320     569     910    1365 
   Size:        320     249     341     455

  Fragments arranged by size: 

                 455     341     320     249
 
BtgI C'CryG_G

Cuts at:      0     879    1365 
   Size:        879     486
 
BtsI GCAGTG_nn'

Cuts at:      0     253     863    1365 
   Size:        253     610     502

  Fragments arranged by size: 

                 610     502     253
 
DraI TTT'AAA

Cuts at:      0      78    1365 
   Size:         78    1287
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      36     890    1365 
   Size:         36     854     475

  Fragments arranged by size: 

                 854     475      36
 
EarI CTCTTCn'nnn_

Cuts at:      0      50     613    1126    1365 
   Size:         50     563     513     239

  Fragments arranged by size: 

                 563     513     239      50
 
EcoICRI GAG'CTC

Cuts at:      0     499    1365 
   Size:        499     866
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     628     679    1232    1304    1355    1365 
   Size:        280     348      51     553      72      51      10

  Fragments arranged by size: 

                 553     348     280      72      51      51      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0     347    1365 
   Size:        347    1018
 
EcoRV GAT'ATC

Cuts at:      0     189    1365 
   Size:        189    1176
 
HaeII r_GCGC'y

Cuts at:      0     230     906    1077    1365 
   Size:        230     676     171     288

  Fragments arranged by size: 

                 676     288     230     171
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     382     414    1365 
   Size:        382      32     951

  Fragments arranged by size: 

                 951     382      32
 
HincII GTy'rAC

Cuts at:      0     454     564    1008    1365 
   Size:        454     110     444     357

  Fragments arranged by size: 

                 454     444     357     110
 
HindIII A'AGCT_T

Cuts at:      0     110    1365 
   Size:        110    1255
 
KasI G'GCGC_C

Cuts at:      0     226    1365 
   Size:        226    1139
 
MluI A'CGCG_T

Cuts at:      0    1203    1365 
   Size:       1203     162
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      52    1192    1365 
   Size:         52    1140     173

  Fragments arranged by size: 

                1140     173      52
 
MslI CAynn'nnrTG

Cuts at:      0     995    1365 
   Size:        995     370
 
NarI GG'CG_CC

Cuts at:      0     227    1365 
   Size:        227    1138
 
NcoI C'CATG_G

Cuts at:      0     879    1365 
   Size:        879     486
 
NruI TCG'CGA

Cuts at:      0    1272    1365 
   Size:       1272      93
 
PasI CC'CwG_GG

Cuts at:      0     342    1365 
   Size:        342    1023
 
PpuMI rG'GwC_Cy

Cuts at:      0     347    1365 
   Size:        347    1018
 
PstI C_TGCA'G

Cuts at:      0     811    1365 
   Size:        811     554
 
SacI G_AGCT'C

Cuts at:      0     501    1365 
   Size:        501     864
 
SalI G'TCGA_C

Cuts at:      0     452    1365 
   Size:        452     913
 
SexAI A'CCwGG_T

Cuts at:      0     758    1158    1365 
   Size:        758     400     207

  Fragments arranged by size: 

                 758     400     207
 
SfcI C'TryA_G

Cuts at:      0     807    1365 
   Size:        807     558
 
SfoI GGC'GCC

Cuts at:      0     228    1365 
   Size:        228    1137
 
SmlI C'TyrA_G

Cuts at:      0     492     587    1365 
   Size:        492      95     778

  Fragments arranged by size: 

                 778     492      95
 
SspI AAT'ATT

Cuts at:      0     721    1365 
   Size:        721     644
 
StyI C'CwwG_G

Cuts at:      0     879     906     932    1031    1365 
   Size:        879      27      26      99     334

  Fragments arranged by size: 

                 879     334      99      27      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      46      76     545    1365 
   Size:         46      30     469     820

  Fragments arranged by size: 

                 820     469      46      30
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     975    1365 
   Size:        975     390
 
XhoI C'TCGA_G

Cuts at:      0     587    1365 
   Size:        587     778
 
XmnI GAAnn'nnTTC

Cuts at:      0     161     390     484     601    1365 
   Size:        161     229      94     117     764

  Fragments arranged by size: 

                 764     229     161     117      94
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI    BciVI    BglII     BpmI    BpuEI     BsaI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BstYI     BtgI     BtsI     DraI     DrdI     EarI  EcoICRI
  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I   HincII  HindIII     KasI
     MluI     MmeI     MslI     NarI     NcoI     NruI     PasI    PpuMI
     PstI     SacI     SalI    SexAI     SfcI     SfoI     SmlI     SspI
     StyI    TaqII     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     BaeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI
    BsaXI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     EaeI     EagI
     EciI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI     HpaI
     KpnI     MfeI     MscI   MspA1I     NaeI     NdeI   NgoMIV     NheI
     NotI     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII    SanDI     SapI     SbfI     ScaI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XmaI     ZraI
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