Restriction map for pF1KB8689
 (Linear) (Six-base) MAPSORT of: KIBB8689.seq  Check: 5910  from: 1  to: 1350



KIBB8689.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     283    1350 
   Size:        283    1067
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     796    1350 
   Size:        796     554
 
AfeI AGC'GCT

Cuts at:      0     412    1350 
   Size:        412     938
 
AflIII A'CryG_T

Cuts at:      0    1187    1350 
   Size:       1187     163
 
AgeI A'CCGG_T

Cuts at:      0    1009    1350 
   Size:       1009     341
 
AleI CACnn'nnGTG

Cuts at:      0     607     841    1350 
   Size:        607     234     509

  Fragments arranged by size: 

                 607     509     234
 
ApaLI G'TGCA_C

Cuts at:      0     252    1350 
   Size:        252    1098
 
AvaI C'yCGr_G

Cuts at:      0     217     424    1054    1250    1350 
   Size:        217     207     630     196     100

  Fragments arranged by size: 

                 630     217     207     196     100
 
BanI G'GyrC_C

Cuts at:      0     553     832    1006    1012    1309    1350 
   Size:        553     279     174       6     297      41

  Fragments arranged by size: 

                 553     297     279     174      41       6
 
BanII G_rGCy'C

Cuts at:      0     716    1350 
   Size:        716     634
 
BbeI G_GCGC'C

Cuts at:      0     557    1313    1350 
   Size:        557     756      37

  Fragments arranged by size: 

                 756     557      37
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1128    1350 
   Size:       1128     222
 
BbvCI CC'TCA_GC

Cuts at:      0    1001    1350 
   Size:       1001     349
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     208     242     822     856    1350 
   Size:        208      34     580      34     494

  Fragments arranged by size: 

                 580     494     208      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     857     950    1350 
   Size:        857      93     400

  Fragments arranged by size: 

                 857     400      93
 
BglII A'GATC_T

Cuts at:      0     428    1350 
   Size:        428     922
 
BlpI GC'TnA_GC

Cuts at:      0     621    1350 
   Size:        621     729
 
Bme1580I G_kGCm'C

Cuts at:      0     256    1009    1017    1350 
   Size:        256     753       8     333

  Fragments arranged by size: 

                 753     333     256       8
 
BmgBI CAC'GTC

Cuts at:      0     182    1161    1350 
   Size:        182     979     189

  Fragments arranged by size: 

                 979     189     182
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     927    1350 
   Size:        927     423
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192    1350 
   Size:        192    1158
 
Bpu10I CC'TnA_GC

Cuts at:      0    1001    1350 
   Size:       1001     349
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136    1350 
   Size:        136    1214
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     937    1051    1350 
   Size:        937     114     299

  Fragments arranged by size: 

                 937     299     114
 
BsaAI yAC'GTr

Cuts at:      0     807    1350 
   Size:        807     543
 
BsaHI Gr'CG_yC

Cuts at:      0     554    1310    1350 
   Size:        554     756      40

  Fragments arranged by size: 

                 756     554      40
 
BsaWI w'CCGG_w

Cuts at:      0    1009    1350 
   Size:       1009     341
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     328     358    1102    1132    1350 
   Size:        328      30     744      30     218

  Fragments arranged by size: 

                 744     328     218      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1135    1350 
   Size:       1135     215
 
BseYI C'CCAG_C

Cuts at:      0     198    1350 
   Size:        198    1152
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     568     610    1025    1216    1350 
   Size:        248     320      42     415     191     134

  Fragments arranged by size: 

                 415     320     248     191     134      42
 
BsiEI CG_ry'CG

Cuts at:      0     305     395     528     722    1350 
   Size:        305      90     133     194     628

  Fragments arranged by size: 

                 628     305     194     133      90
 
BsiHKAI G_wGCw'C

Cuts at:      0     256     716    1350 
   Size:        256     460     634

  Fragments arranged by size: 

                 634     460     256
 
Bsp1286I G_dGCh'C

Cuts at:      0     256     716    1009    1017    1350 
   Size:        256     460     293       8     333

  Fragments arranged by size: 

                 460     333     293     256       8
 
BspHI T'CATG_A

Cuts at:      0     326    1350 
   Size:        326    1024
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     283     547     582    1265    1350 
   Size:        283     264      35     683      85

  Fragments arranged by size: 

                 683     283     264      85      35
 
BsrBI CCG'CTC

Cuts at:      0     564    1323    1350 
   Size:        564     759      27

  Fragments arranged by size: 

                 759     564      27
 
BsrFI r'CCGG_y

Cuts at:      0     390    1009    1104    1350 
   Size:        390     619      95     246

  Fragments arranged by size: 

                 619     390     246      95
 
BstEII G'GTnAC_C

Cuts at:      0     283     594    1350 
   Size:        283     311     756

  Fragments arranged by size: 

                 756     311     283
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     843    1350 
   Size:        843     507
 
BstYI r'GATC_y

Cuts at:      0     146     428     812    1350 
   Size:        146     282     384     538

  Fragments arranged by size: 

                 538     384     282     146
 
Bsu36I CC'TnA_GG

Cuts at:      0     511    1350 
   Size:        511     839
 
BtgI C'CryG_G

Cuts at:      0     309    1079    1151    1350 
   Size:        309     770      72     199

  Fragments arranged by size: 

                 770     309     199      72
 
BtsI GCAGTG_nn'

Cuts at:      0     629    1350 
   Size:        629     721
 
EaeI y'GGCC_r

Cuts at:      0      91     302     392     464     559     626     719    1082 
   Size:         91     211      90      72      95      67      93     363

Cuts at:   1082    1175    1350 
   Size:         93     175

  Fragments arranged by size: 

                 363     211     175      95      93      93      91      90
                  72      67
 
EagI C'GGCC_G

Cuts at:      0     302     392     719    1350 
   Size:        302      90     327     631

  Fragments arranged by size: 

                 631     327     302      90
 
EarI CTCTTCn'nnn_

Cuts at:      0     439     499    1350 
   Size:        439      60     851

  Fragments arranged by size: 

                 851     439      60
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     276    1350 
   Size:        276    1074
 
EcoICRI GAG'CTC

Cuts at:      0     714    1350 
   Size:        714     636
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     796    1350 
   Size:        192     604     554

  Fragments arranged by size: 

                 604     554     192
 
EcoO109I rG'GnC_Cy

Cuts at:      0     321     501    1113    1254    1350 
   Size:        321     180     612     141      96

  Fragments arranged by size: 

                 612     321     180     141      96
 
FspI TGC'GCA

Cuts at:      0     238     379    1350 
   Size:        238     141     971

  Fragments arranged by size: 

                 971     238     141
 
HaeII r_GCGC'y

Cuts at:      0     179     414     557    1313    1350 
   Size:        179     235     143     756      37

  Fragments arranged by size: 

                 756     235     179     143      37
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     778     810    1350 
   Size:        778      32     540

  Fragments arranged by size: 

                 778     540      32
 
KasI G'GCGC_C

Cuts at:      0     553    1309    1350 
   Size:        553     756      41

  Fragments arranged by size: 

                 756     553      41
 
MluI A'CGCG_T

Cuts at:      0    1187    1350 
   Size:       1187     163
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1172    1241    1350 
   Size:       1172      69     109

  Fragments arranged by size: 

                1172     109      69
 
MscI TGG'CCA

Cuts at:      0    1084    1350 
   Size:       1084     266
 
MslI CAynn'nnrTG

Cuts at:      0     607     841     844     995    1238    1350 
   Size:        607     234       3     151     243     112

  Fragments arranged by size: 

                 607     243     234     151     112       3
 
MspA1I CmG'CkG

Cuts at:      0     625    1350 
   Size:        625     725
 
NarI GG'CG_CC

Cuts at:      0     554    1310    1350 
   Size:        554     756      40

  Fragments arranged by size: 

                 756     554      40
 
NcoI C'CATG_G

Cuts at:      0     309    1350 
   Size:        309    1041
 
NspI r_CATG'y

Cuts at:      0     888    1350 
   Size:        888     462
 
PasI CC'CwG_GG

Cuts at:      0      81    1350 
   Size:         81    1269
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     807    1350 
   Size:        807     543
 
PfoI T'CCnGG_A

Cuts at:      0     668    1350 
   Size:        668     682
 
PmlI CAC'GTG

Cuts at:      0     807    1350 
   Size:        807     543
 
PpuMI rG'GwC_Cy

Cuts at:      0     321     501    1113    1254    1350 
   Size:        321     180     612     141      96

  Fragments arranged by size: 

                 612     321     180     141      96
 
PstI C_TGCA'G

Cuts at:      0     118    1350 
   Size:        118    1232
 
SacI G_AGCT'C

Cuts at:      0     716    1350 
   Size:        716     634
 
SanDI GG'GwC_CC

Cuts at:      0     501    1350 
   Size:        501     849
 
SapI GCTCTTCn'nnn_

Cuts at:      0     439    1350 
   Size:        439     911
 
SfcI C'TryA_G

Cuts at:      0     114    1350 
   Size:        114    1236
 
SfoI GGC'GCC

Cuts at:      0     555    1311    1350 
   Size:        555     756      39

  Fragments arranged by size: 

                 756     555      39
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1009    1350 
   Size:       1009     341
 
SmlI C'TyrA_G

Cuts at:      0     151    1350 
   Size:        151    1199
 
SphI G_CATG'C

Cuts at:      0     888    1350 
   Size:        888     462
 
StuI AGG'CCT

Cuts at:      0     985    1129    1350 
   Size:        985     144     221

  Fragments arranged by size: 

                 985     221     144
 
StyI C'CwwG_G

Cuts at:      0      10     309     347    1350 
   Size:         10     299      38    1003

  Fragments arranged by size: 

                1003     299      38      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1059    1350 
   Size:       1059     291
 
TatI w'GTAC_w

Cuts at:      0     824    1118    1350 
   Size:        824     294     232

  Fragments arranged by size: 

                 824     294     232
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     328     360    1350 
   Size:        328      32     990

  Fragments arranged by size: 

                 990     328      32
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AgeI     AleI    ApaLI     AvaI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI   BstEII    BstXI
    BstYI   Bsu36I     BtgI     BtsI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     FspI    HaeII    Hin4I     KasI     MluI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NspI     PasI
    PflMI     PfoI     PmlI    PpuMI     PstI     SacI    SanDI     SapI
     SfcI     SfoI    SgrAI     SmlI     SphI     StuI     StyI    TaqII
     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AhdI     AlfI     AloI
    AlwNI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BciVI     BclI    BfrBI     BmtI     BplI    BsaBI    BsiWI
     BsmI    BsmBI    BspEI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI
     SbfI     ScaI    SexAI     SfiI     SgfI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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