Restriction map for pF1KB8688
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
AlwI        AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
Sau3AI      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Tth111I     XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          104  GTCTAC               GT^[AC][GT]AC
Alw44I       1044  GTGCAC               G^TGCAC
AlwNI        1156  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        1044  GTGCAC               G^TGCAC
Asp700I      1305  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII         934  GAGCTC               G[GA]GC[CT]^C
BbvI          372  GCAGC                GCAGC(8/12)
BfaI          667  CTAG                 C^TAG
BpmI          505  CTGGAG               CTGGAG(16/14)
BsaBI         457  GATTTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         634  GAGGAG               GAGGAG(10/8)
BsgI           20  GTGCAG               GTGCAG(16/14)
BsiWI        1214  CGTACG               C^GTACG
BsmBI        1189  GAGACG               CGTCTC(1/5)
BsrDI         881  GCAATG               GCAATG(2/0)
BstAPI        866  GCACATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1083  GGTCACC              G^GT[ACGT]ACC
BstPI        1083  GGTCACC              G^GT[ACGT]ACC
DraII         638  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI         1079  CCACGG               C^C[GA][CT]GG
EcoO109I      638  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1083  GGTCACC              G^GT[ACGT]ACC
EcoT14I       650  CCTTGG               C^C[AT][AT]GG
FauI          228  GCGGG                CCCGC(4/6)
HincII       1198  GTCAAC               GT[CT]^[GA]AC
HindII       1198  GTCAAC               GT[CT]^[GA]AC
MaeI          667  CTAG                 C^TAG
MaeII         889  ACGT                 A^CGT
MamI          457  GATTTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I       1128  CAGCTG               C[AC]G^C[GT]G
NspI         1121  ACATGC               [GA]CATG^[CT]
PpuMI         638  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI        1191  GACGGTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        1128  CAGCTG               CAG^CTG
SacI          934  GAGCTC               GAGCT^C
SapI          532  GAAGAGC              GCTCTTC(1/4)
StuI         1139  AGGCCT               AGG^CCT
StyI          650  CCTTGG               C^C[AT][AT]GG
TseI          372  GCAGC                G^C[AT]GC
XmnI         1305  GAAGGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AvaI          398  CTCGGG               C^[CT]CG[GA]G
             1490  CTCGGG              
BglI          284  GCCCAGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              841  GCCAAGCTGGC         
BsmAI          74  GAGAC                GTCTC(1/5)
             1189  GAGAC               
BsoBI         398  CTCGGG               C^[CT]CG[GA]G
             1490  CTCGGG              
BspMI         771  ACCTGC               ACCTGC(4/8)
              138  GCAGGT              
BsrBI         226  GAGCGG               CCGCTC(-3/-3)
             1174  GAGCGG              
EcoRI         270  GAATTC               G^AATTC
              897  GAATTC              
Fnu4HI        372  GCAGC                GC^[ACGT]GC
             1176  GCGGC               
HaeIII        283  GGCC                 GG^CC
             1140  GGCC                
HphI         1060  TCACC                GGTGA(8/7)
             1085  TCACC               
ItaI          372  GCAGC                GC^[ACGT]GC
             1176  GCGGC               
MslI          987  CACAGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1450  CACAACAATG          
PflMI         185  CCATTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1066  CCAAATACTGG         
PleI          318  GACTC                GAGTC(4/5)
              814  GACTC               
PstI          773  CTGCAG               CTGCA^G
             1153  CTGCAG              
SfcI          773  CTGCAG               C^T[GA][CT]AG
             1153  CTGCAG              
SspI          544  AATATT               AAT^ATT
             1255  AATATT              
TfiI         1054  GAATC                G^A[AT]TC
             1266  GATTC               
Van91I        185  CCATTGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1066  CCAAATACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AspHI       AvaII       BbsI        BmyI        BpuAI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        EarI        EcoRII      
FokI        HinfI       Ksp632I     MaeIII      MboII       
MnlI        MseI        MvaI        MwoI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       SfaNI       
TaqI        Tru9I       Tsp45I      Tsp509I     TspRI       
TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com