Restriction map for pF1KB8688
 (Linear) (Six-base) MAPSORT of: KIBB8688.seq  Check: 1600  from: 1  to: 1506



KIBB8688.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AccI GT'mk_AC

Cuts at:      0     105    1506 
   Size:        105    1401
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218    1366    1506 
   Size:        218    1148     140

  Fragments arranged by size: 

                1148     218     140
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1009    1506 
   Size:       1009     497
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1161    1506 
   Size:       1161     345
 
ApaLI G'TGCA_C

Cuts at:      0    1044    1506 
   Size:       1044     462
 
ApoI r'AATT_y

Cuts at:      0      79     270     897    1396    1506 
   Size:         79     191     627     499     110

  Fragments arranged by size: 

                 627     499     191     110      79
 
AvaI C'yCGr_G

Cuts at:      0     398    1490    1506 
   Size:        398    1092      16

  Fragments arranged by size: 

                1092     398      16
 
BanII G_rGCy'C

Cuts at:      0     938    1506 
   Size:        938     568
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     494     737     841    1484    1506 
   Size:        494     243     104     643      22

  Fragments arranged by size: 

                 643     494     243     104      22
 
BglI GCCn_nnn'nGGC

Cuts at:      0     290     847    1506 
   Size:        290     557     659

  Fragments arranged by size: 

                 659     557     290
 
Bme1580I G_kGCm'C

Cuts at:      0    1048    1118    1506 
   Size:       1048      70     388

  Fragments arranged by size: 

                1048     388      70
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     526    1506 
   Size:        526     980
 
BsaBI GATnn'nnATC

Cuts at:      0     461    1506 
   Size:        461    1045
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     649    1506 
   Size:        649     857
 
BseYI C'CCAG_C

Cuts at:      0    1126    1168    1506 
   Size:       1126      42     338

  Fragments arranged by size: 

                1126     338      42
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41    1506 
   Size:         41    1465
 
BsiHKAI G_wGCw'C

Cuts at:      0     856     938    1048    1506 
   Size:        856      82     110     458

  Fragments arranged by size: 

                 856     458     110      82
 
BsiWI C'GTAC_G

Cuts at:      0    1214    1506 
   Size:       1214     292
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1183    1506 
   Size:       1183     323
 
Bsp1286I G_dGCh'C

Cuts at:      0     856     938    1048    1118    1506 
   Size:        856      82     110      70     388

  Fragments arranged by size: 

                 856     388     110      82      70
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     129     780    1506 
   Size:        129     651     726

  Fragments arranged by size: 

                 726     651     129
 
BsrBI CCG'CTC

Cuts at:      0     228    1176    1506 
   Size:        228     948     330

  Fragments arranged by size: 

                 948     330     228
 
BsrDI GCAATG_nn'

Cuts at:      0     888    1506 
   Size:        888     618
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     872    1506 
   Size:        872     634
 
BstEII G'GTnAC_C

Cuts at:      0    1083    1506 
   Size:       1083     423
 
BtgI C'CryG_G

Cuts at:      0    1079    1506 
   Size:       1079     427
 
BtsI GCAGTG_nn'

Cuts at:      0     930    1186    1506 
   Size:        930     256     320

  Fragments arranged by size: 

                 930     320     256
 
EarI CTCTTCn'nnn_

Cuts at:      0     194     527     686    1325    1506 
   Size:        194     333     159     639     181

  Fragments arranged by size: 

                 639     333     194     181     159
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     469    1506 
   Size:        469    1037
 
EcoICRI GAG'CTC

Cuts at:      0     936    1506 
   Size:        936     570
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     526    1366    1506 
   Size:        218     308     840     140

  Fragments arranged by size: 

                 840     308     218     140
 
EcoO109I rG'GnC_Cy

Cuts at:      0     639    1506 
   Size:        639     867
 
EcoRI G'AATT_C

Cuts at:      0     270     897    1506 
   Size:        270     627     609

  Fragments arranged by size: 

                 627     609     270
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1042    1074    1506 
   Size:       1042      32     432

  Fragments arranged by size: 

                1042     432      32
 
HincII GTy'rAC

Cuts at:      0    1200    1506 
   Size:       1200     306
 
MslI CAynn'nnrTG

Cuts at:      0     991    1454    1506 
   Size:        991     463      52

  Fragments arranged by size: 

                 991     463      52
 
MspA1I CmG'CkG

Cuts at:      0    1130    1506 
   Size:       1130     376
 
NspI r_CATG'y

Cuts at:      0    1125    1506 
   Size:       1125     381
 
PasI CC'CwG_GG

Cuts at:      0     602    1506 
   Size:        602     904
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     191    1072    1506 
   Size:        191     881     434

  Fragments arranged by size: 

                 881     434     191
 
PpuMI rG'GwC_Cy

Cuts at:      0     639    1506 
   Size:        639     867
 
PshAI GACnn'nnGTC

Cuts at:      0    1195    1506 
   Size:       1195     311
 
PsiI TTA'TAA

Cuts at:      0     703    1506 
   Size:        703     803
 
PstI C_TGCA'G

Cuts at:      0     777    1157    1506 
   Size:        777     380     349

  Fragments arranged by size: 

                 777     380     349
 
PvuII CAG'CTG

Cuts at:      0    1130    1506 
   Size:       1130     376
 
SacI G_AGCT'C

Cuts at:      0     938    1506 
   Size:        938     568
 
SapI GCTCTTCn'nnn_

Cuts at:      0     527    1506 
   Size:        527     979
 
SfcI C'TryA_G

Cuts at:      0     773    1153    1506 
   Size:        773     380     353

  Fragments arranged by size: 

                 773     380     353
 
SspI AAT'ATT

Cuts at:      0     546    1257    1506 
   Size:        546     711     249

  Fragments arranged by size: 

                 711     546     249
 
StuI AGG'CCT

Cuts at:      0    1141    1506 
   Size:       1141     365
 
StyI C'CwwG_G

Cuts at:      0     650    1506 
   Size:        650     856
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       7     931    1506 
   Size:          7     924     575

  Fragments arranged by size: 

                 924     575       7
 
TatI w'GTAC_w

Cuts at:      0     153     325    1506 
   Size:        153     172    1181

  Fragments arranged by size: 

                1181     172     153
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     223     255    1506 
   Size:        223      32    1251

  Fragments arranged by size: 

                1251     223      32
 
XmnI GAAnn'nnTTC

Cuts at:      0    1309    1506 
   Size:       1309     197
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI    ApaLI     ApoI     AvaI    BanII
     BbsI     BglI Bme1580I     BpmI    BsaBI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI   BstAPI
   BstEII     BtgI     BtsI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI    Hin4I   HincII     MslI   MspA1I     NspI     PasI    PflMI
    PpuMI    PshAI     PsiI     PstI    PvuII     SacI     SapI     SfcI
     SspI     StuI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BsiEI     BsmI
    BspEI    BspHI    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI     PmlI
     PpiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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