Restriction map for pF1KB8687
 (Linear) (Six-base) MAPSORT of: KIBB8687.seq  Check: 6804  from: 1  to: 1944



KIBB8687.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1180    1944 
   Size:       1180     764
 
AflIII A'CryG_T

Cuts at:      0    1671    1944 
   Size:       1671     273
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1654    1944 
   Size:       1654     290
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     373    1944 
   Size:        373    1571
 
AlwNI CAG_nnn'CTG

Cuts at:      0     118    1944 
   Size:        118    1826
 
ApoI r'AATT_y

Cuts at:      0     338     657    1944 
   Size:        338     319    1287

  Fragments arranged by size: 

                1287     338     319
 
AseI AT'TA_AT

Cuts at:      0    1808    1944 
   Size:       1808     136
 
AvaI C'yCGr_G

Cuts at:      0      71     974    1944 
   Size:         71     903     970

  Fragments arranged by size: 

                 970     903      71
 
BanI G'GyrC_C

Cuts at:      0     157    1944 
   Size:        157    1787
 
BanII G_rGCy'C

Cuts at:      0      21    1944 
   Size:         21    1923
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1467    1944 
   Size:       1467     477
 
BglII A'GATC_T

Cuts at:      0     840    1944 
   Size:        840    1104
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171    1944 
   Size:        171    1773
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1904    1944 
   Size:       1904      40
 
BsaAI yAC'GTr

Cuts at:      0      35    1713    1944 
   Size:         35    1678     231

  Fragments arranged by size: 

                1678     231      35
 
BsaBI GATnn'nnATC

Cuts at:      0     518    1944 
   Size:        518    1426
 
BsaHI Gr'CG_yC

Cuts at:      0     734    1944 
   Size:        734    1210
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1026    1056    1737    1767    1944 
   Size:       1026      30     681      30     177

  Fragments arranged by size: 

                1026     681     177      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      29      99     112    1858    1944 
   Size:         29      70      13    1746      86

  Fragments arranged by size: 

                1746      86      70      29      13
 
BseYI C'CCAG_C

Cuts at:      0     922    1944 
   Size:        922    1022
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     705    1944 
   Size:        705    1239
 
BsiEI CG_ry'CG

Cuts at:      0     252    1944 
   Size:        252    1692
 
BsiHKAI G_wGCw'C

Cuts at:      0      21    1944 
   Size:         21    1923
 
BsmI GAATG_Cn'

Cuts at:      0     430     442    1944 
   Size:        430      12    1502

  Fragments arranged by size: 

                1502     430      12
 
Bsp1286I G_dGCh'C

Cuts at:      0      21    1944 
   Size:         21    1923
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     727     769    1944 
   Size:        727      42    1175

  Fragments arranged by size: 

                1175     727      42
 
BsrDI GCAATG_nn'

Cuts at:      0     362    1944 
   Size:        362    1582
 
BsrFI r'CCGG_y

Cuts at:      0      90    1944 
   Size:         90    1854
 
BssSI C'ACGA_G

Cuts at:      0     193    1944 
   Size:        193    1751
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     235    1944 
   Size:        235    1709
 
BstYI r'GATC_y

Cuts at:      0     840    1359    1944 
   Size:        840     519     585

  Fragments arranged by size: 

                 840     585     519
 
Bsu36I CC'TnA_GG

Cuts at:      0     119     752    1944 
   Size:        119     633    1192

  Fragments arranged by size: 

                1192     633     119
 
BtgI C'CryG_G

Cuts at:      0       9     179    1693    1944 
   Size:          9     170    1514     251

  Fragments arranged by size: 

                1514     251     170       9
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1288    1944 
   Size:       1288     656
 
BtsI GCAGTG_nn'

Cuts at:      0     383     605    1944 
   Size:        383     222    1339

  Fragments arranged by size: 

                1339     383     222
 
DraI TTT'AAA

Cuts at:      0    1599    1944 
   Size:       1599     345
 
EaeI y'GGCC_r

Cuts at:      0     182     502    1944 
   Size:        182     320    1442

  Fragments arranged by size: 

                1442     320     182
 
EarI CTCTTCn'nnn_

Cuts at:      0     217    1073    1406    1731    1944 
   Size:        217     856     333     325     213

  Fragments arranged by size: 

                 856     333     325     217     213
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     109    1944 
   Size:        109    1835
 
EcoICRI GAG'CTC

Cuts at:      0      19    1944 
   Size:         19    1925
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171    1180    1944 
   Size:        171    1009     764

  Fragments arranged by size: 

                1009     764     171
 
EcoO109I rG'GnC_Cy

Cuts at:      0     115     167    1944 
   Size:        115      52    1777

  Fragments arranged by size: 

                1777     115      52
 
EcoRV GAT'ATC

Cuts at:      0    1101    1944 
   Size:       1101     843
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     246     606    1944 
   Size:        246     360    1338

  Fragments arranged by size: 

                1338     360     246
 
HaeII r_GCGC'y

Cuts at:      0      79    1944 
   Size:         79    1865
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     116     148    1492    1524    1624    1640    1656    1672 
   Size:        116      32    1344      32     100      16      16      16

Cuts at:   1672    1837    1869    1944 
   Size:        165      32      75

  Fragments arranged by size: 

                1344     165     116     100      75      32      32      32
                  16      16      16
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     438     471     625    1184    1944 
   Size:        438      33     154     559     760

  Fragments arranged by size: 

                 760     559     438     154      33
 
MslI CAynn'nnrTG

Cuts at:      0     813    1944 
   Size:        813    1131
 
MspA1I CmG'CkG

Cuts at:      0     187     207    1944 
   Size:        187      20    1737

  Fragments arranged by size: 

                1737     187      20
 
NaeI GCC'GGC

Cuts at:      0      92    1944 
   Size:         92    1852
 
NgoMIV G'CCGG_C

Cuts at:      0      90    1944 
   Size:         90    1854
 
NspI r_CATG'y

Cuts at:      0     957    1431    1675    1944 
   Size:        957     474     244     269

  Fragments arranged by size: 

                 957     474     269     244
 
PciI A'CATG_T

Cuts at:      0    1671    1944 
   Size:       1671     273
 
PfoI T'CCnGG_A

Cuts at:      0    1116    1944 
   Size:       1116     828
 
PpuMI rG'GwC_Cy

Cuts at:      0     115    1944 
   Size:        115    1829
 
PsiI TTA'TAA

Cuts at:      0     596    1944 
   Size:        596    1348
 
SacI G_AGCT'C

Cuts at:      0      21    1944 
   Size:         21    1923
 
SapI GCTCTTCn'nnn_

Cuts at:      0     217    1073    1731    1944 
   Size:        217     856     658     213

  Fragments arranged by size: 

                 856     658     217     213
 
SfcI C'TryA_G

Cuts at:      0    1441    1944 
   Size:       1441     503
 
SmlI C'TyrA_G

Cuts at:      0    1919    1944 
   Size:       1919      25
 
SnaBI TAC'GTA

Cuts at:      0    1713    1944 
   Size:       1713     231
 
SspI AAT'ATT

Cuts at:      0    1015    1701    1812    1944 
   Size:       1015     686     111     132

  Fragments arranged by size: 

                1015     686     132     111
 
StuI AGG'CCT

Cuts at:      0      28    1944 
   Size:         28    1916
 
StyI C'CwwG_G

Cuts at:      0      23    1944 
   Size:         23    1921
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     238    1944 
   Size:        238    1706
 
TatI w'GTAC_w

Cuts at:      0     283    1944 
   Size:        283    1661
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1026    1058    1737    1769    1944 
   Size:       1026      32     679      32     175

  Fragments arranged by size: 

                1026     679     175      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      66    1465    1944 
   Size:         66    1399     479

  Fragments arranged by size: 

                1399     479      66
 
XbaI T'CTAG_A

Cuts at:      0    1642    1944 
   Size:       1642     302
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     454     512    1944 
   Size:        454      58    1432

  Fragments arranged by size: 

                1432     454      58
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     AlfI    AlwNI     ApoI     AseI     AvaI
     BanI    BanII     BbsI    BglII     BpmI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrDI    BsrFI    BssSI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI     EaeI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRV     FalI    HaeII    Hin4I     MmeI     MslI
   MspA1I     NaeI   NgoMIV     NspI     PciI     PfoI    PpuMI     PsiI
     SacI     SapI     SfcI     SmlI    SnaBI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI
    BsaWI    BsiWI    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I     ClaI    CspCI   DraIII     DrdI
     EagI    EcoNI    EcoRI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI     SphI
     SrfI     SwaI     XhoI     XmaI     XmnI     ZraI
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