Restriction map for pF1KB8677
 (Linear) (Six-base) MAPSORT of: KIBB8677.seq  Check: 7930  from: 1  to: 1104



KIBB8677.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AccI GT'mk_AC

Cuts at:      0     127    1104 
   Size:        127     977
 
AflIII A'CryG_T

Cuts at:      0     723     776     986    1104 
   Size:        723      53     210     118

  Fragments arranged by size: 

                 723     210     118      53
 
AlwNI CAG_nnn'CTG

Cuts at:      0      44     408     460    1104 
   Size:         44     364      52     644

  Fragments arranged by size: 

                 644     364      52      44
 
ApaI G_GGCC'C

Cuts at:      0      27      72     498    1104 
   Size:         27      45     426     606

  Fragments arranged by size: 

                 606     426      45      27
 
ApoI r'AATT_y

Cuts at:      0     768    1104 
   Size:        768     336
 
AvaI C'yCGr_G

Cuts at:      0     638    1104 
   Size:        638     466
 
BanI G'GyrC_C

Cuts at:      0     146     207     871    1104 
   Size:        146      61     664     233

  Fragments arranged by size: 

                 664     233     146      61
 
BanII G_rGCy'C

Cuts at:      0      27      72     498     689    1104 
   Size:         27      45     426     191     415

  Fragments arranged by size: 

                 426     415     191      45      27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     542    1084    1104 
   Size:        542     542      20

  Fragments arranged by size: 

                 542     542      20
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     808    1104 
   Size:        808     296
 
BclI T'GATC_A

Cuts at:      0     561    1104 
   Size:        561     543
 
BglI GCCn_nnn'nGGC

Cuts at:      0      22    1104 
   Size:         22    1082
 
BglII A'GATC_T

Cuts at:      0     445    1104 
   Size:        445     659
 
Bme1580I G_kGCm'C

Cuts at:      0      27      72     498     986     997    1104 
   Size:         27      45     426     488      11     107

  Fragments arranged by size: 

                 488     426     107      45      27      11
 
BmgBI CAC'GTC

Cuts at:      0     960    1104 
   Size:        960     144
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      74    1104 
   Size:         74    1030
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     317     703     870    1104 
   Size:        128     189     386     167     234

  Fragments arranged by size: 

                 386     234     189     167     128
 
Bpu10I CC'TnA_GC

Cuts at:      0     649    1104 
   Size:        649     455
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     226    1104 
   Size:        226     878
 
BsaAI yAC'GTr

Cuts at:      0     724     777    1104 
   Size:        724      53     327

  Fragments arranged by size: 

                 724     327      53
 
BsaBI GATnn'nnATC

Cuts at:      0     698    1104 
   Size:        698     406
 
BsiEI CG_ry'CG

Cuts at:      0      58     101    1104 
   Size:         58      43    1003

  Fragments arranged by size: 

                1003      58      43
 
BsiHKAI G_wGCw'C

Cuts at:      0     689    1104 
   Size:        689     415
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     637     966    1104 
   Size:        637     329     138

  Fragments arranged by size: 

                 637     329     138
 
Bsp1286I G_dGCh'C

Cuts at:      0      27      72     498     689     986     997    1104 
   Size:         27      45     426     191     297      11     107

  Fragments arranged by size: 

                 426     297     191     107      45      27      11
 
BsrBI CCG'CTC

Cuts at:      0      87     843    1104 
   Size:         87     756     261

  Fragments arranged by size: 

                 756     261      87
 
BsrGI T'GTAC_A

Cuts at:      0      10    1104 
   Size:         10    1094
 
BssSI C'ACGA_G

Cuts at:      0     419    1104 
   Size:        419     685
 
BstYI r'GATC_y

Cuts at:      0     445    1104 
   Size:        445     659
 
Bsu36I CC'TnA_GG

Cuts at:      0    1089    1104 
   Size:       1089      15
 
BtgI C'CryG_G

Cuts at:      0      32     360    1104 
   Size:         32     328     744

  Fragments arranged by size: 

                 744     328      32
 
EaeI y'GGCC_r

Cuts at:      0      29      76      98     357    1104 
   Size:         29      47      22     259     747

  Fragments arranged by size: 

                 747     259      47      29      22
 
EagI C'GGCC_G

Cuts at:      0      98    1104 
   Size:         98    1006
 
EcoICRI GAG'CTC

Cuts at:      0     687    1104 
   Size:        687     417
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     317     703     870    1104 
   Size:        128     189     386     167     234

  Fragments arranged by size: 

                 386     234     189     167     128
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24      68     495     653    1104 
   Size:         24      44     427     158     451

  Fragments arranged by size: 

                 451     427     158      44      24
 
EcoRI G'AATT_C

Cuts at:      0     768    1104 
   Size:        768     336
 
HaeII r_GCGC'y

Cuts at:      0     792    1104 
   Size:        792     312
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     263     295     402     434     846     878    1104 
   Size:        263      32     107      32     412      32     226

  Fragments arranged by size: 

                 412     263     226     107      32      32      32
 
HincII GTy'rAC

Cuts at:      0     128    1104 
   Size:        128     976
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     600    1104 
   Size:        164     436     504

  Fragments arranged by size: 

                 504     436     164
 
MscI TGG'CCA

Cuts at:      0      78     359    1104 
   Size:         78     281     745

  Fragments arranged by size: 

                 745     281      78
 
MslI CAynn'nnrTG

Cuts at:      0     751    1031    1104 
   Size:        751     280      73

  Fragments arranged by size: 

                 751     280      73
 
MspA1I CmG'CkG

Cuts at:      0      44    1104 
   Size:         44    1060
 
NcoI C'CATG_G

Cuts at:      0     360    1104 
   Size:        360     744
 
NspI r_CATG'y

Cuts at:      0     990    1104 
   Size:        990     114
 
PciI A'CATG_T

Cuts at:      0     986    1104 
   Size:        986     118
 
PfoI T'CCnGG_A

Cuts at:      0     836    1104 
   Size:        836     268
 
PmlI CAC'GTG

Cuts at:      0     777    1104 
   Size:        777     327
 
PspOMI G'GGCC_C

Cuts at:      0      23      68     494    1104 
   Size:         23      45     426     610

  Fragments arranged by size: 

                 610     426      45      23
 
PstI C_TGCA'G

Cuts at:      0     330     971    1104 
   Size:        330     641     133

  Fragments arranged by size: 

                 641     330     133
 
SacI G_AGCT'C

Cuts at:      0     689    1104 
   Size:        689     415
 
SalI G'TCGA_C

Cuts at:      0     126    1104 
   Size:        126     978
 
SfcI C'TryA_G

Cuts at:      0     326     967    1104 
   Size:        326     641     137

  Fragments arranged by size: 

                 641     326     137
 
StuI AGG'CCT

Cuts at:      0    1094    1104 
   Size:       1094      10
 
StyI C'CwwG_G

Cuts at:      0      79     360     368     875     926    1104 
   Size:         79     281       8     507      51     178

  Fragments arranged by size: 

                 507     281     178      79      51       8
 
TatI w'GTAC_w

Cuts at:      0      10     779    1104 
   Size:         10     769     325

  Fragments arranged by size: 

                 769     325      10
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     139     171    1104 
   Size:        139      32     933

  Fragments arranged by size: 

                 933     139      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     523    1104 
   Size:        523     581
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     513    1104 
   Size:        513     591
 
XmnI GAAnn'nnTTC

Cuts at:      0     596    1104 
   Size:        596     508
 

 Enzymes that do cut:

     AccI   AflIII    AlwNI     ApaI     ApoI     AvaI     BanI    BanII
     BbsI    BciVI     BclI     BglI    BglII Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BsrBI    BsrGI    BssSI    BstYI   Bsu36I     BtgI     EaeI
     EagI  EcoICRI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I   HincII
     MmeI     MscI     MslI   MspA1I     NcoI     NspI     PciI     PfoI
     PmlI   PspOMI     PstI     SacI     SalI     SfcI     StuI     StyI
     TatI     TstI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI    BfrBI     BlpI
     BmtI     BplI    BpuEI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrDI    BsrFI
   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EarI     EciI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmeI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     XbaI     XhoI     XmaI     ZraI
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