Restriction map for
pF1KB8674
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlpI Bpu1102I BsaBI BsaHI
BsaI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoT22I FauI
FbaI FseI FspI HaeII HaeIII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MslI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AflIII 177 ACATGT A^C[GA][CT]GT
AlwI 922 GATCC GGATC(4/5)
Asp700I 352 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1257 CCTAGG C^CTAGG
BanII 896 GAGCCC G[GA]GC[CT]^C
BbsI 502 GAAGAC GAAGAC(2/6)
BfaI 1258 CTAG C^TAG
BglII 1285 AGATCT A^GATCT
BlnI 1257 CCTAGG C^CTAGG
BmyI 896 GAGCCC G[AGT]GC[ACT]^C
BpuAI 502 GAAGAC GAAGAC(2/6)
BsaAI 278 TACGTG [CT]AC^GT[GA]
BsaJI 1257 CCTAGG C^C[ACGT][ACGT]GG
BsaWI 1509 TCCGGT [AT]^CCGG[AT]
Bsp1286I 896 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 177 ACATGT A^CATGT
BssKI 1062 CCTGG ^CC[ACGT]GG
BstAPI 1321 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 1062 CCTGG CC^[AT]GG
DraIII 1196 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 200 CTCTTC CTCTTC(1/4)
EcoNI 430 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1305 GAATTC G^AATTC
EcoRII 1062 CCTGG ^CC[AT]GG
EcoT14I 1257 CCTAGG C^C[AT][AT]GG
HapII 1510 CCGG C^CGG
HpaII 1510 CCGG C^CGG
Ksp632I 200 CTCTTC CTCTTC(1/4)
MaeI 1258 CTAG C^TAG
MfeI 711 CAATTG C^AATTG
MspA1I 455 CAGCTG C[AC]G^C[GT]G
MspI 1510 CCGG C^CGG
MunI 711 CAATTG C^AATTG
MvaI 1062 CCTGG CC^[AT]GG
NdeI 93 CATATG CA^TATG
NspI 177 ACATGT [GA]CATG^[CT]
PstI 901 CTGCAG CTGCA^G
PvuII 455 CAGCTG CAG^CTG
SbfI 900 CCTGCAGG CCTGCA^GG
ScaI 112 AGTACT AGT^ACT
ScrFI 1062 CCTGG CC^[ACGT]GG
Sse8387I 900 CCTGCAGG CCTGCA^GG
StyI 1257 CCTAGG C^C[AT][AT]GG
TfiI 931 GATTC G^A[AT]TC
XmnI 352 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 14 CCGC CCGC(-3/-1)
799 GCGG
BpmI 431 CTCCAG CTGGAG(16/14)
1136 CTCCAG
BsmAI 787 GAGAC GTCTC(1/5)
451 GTCTC
BsmI 1029 GCATTC GAATGC(1/-1)
1223 GCATTC
BstYI 921 AGATCC [GA]^GATC[CT]
1285 AGATCT
DraI 667 TTTAAA TTT^AAA
777 TTTAAA
DraII 365 AGGACCC [GA]G^G[ACGT]CC[CT]
1260 AGGACCC
EcoO109I 365 AGGACCC [GA]G^G[ACGT]CC[CT]
1260 AGGACCC
EcoRV 384 GATATC GAT^ATC
970 GATATC
MflI 921 AGATCC [GA]^GATC[CT]
1285 AGATCT
PflMI 710 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
978 CCAACAGATGG
PleI 943 GACTC GAGTC(4/5)
78 GAGTC
PpuMI 365 AGGACCC [GA]G^G[AT]CC[CT]
1260 AGGACCC
SfaNI 706 GATGC GCATC(5/9)
1156 GCATC
TaqI 26 TCGA T^CGA
481 TCGA
TthHB8I 26 TCGA T^CGA
481 TCGA
Van91I 710 CCAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
978 CCAACAGATGG
XhoII 921 AGATCC [GA]^GATC[CT]
1285 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
AvaII BbvI BsiYI BslI BspMI
BsrI BstF5I Cac8I Cfr13I DdeI
DpnII Fnu4HI FokI HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I SfcI
Tru9I TseI Tsp45I Tsp509I TspRI