Restriction map for pF1KB8663
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoRI       EcoRV       FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MslI        
MspA1I      MunI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII        1634  CGCG                 CG^CG
AocI         1549  CCTGAGG              CC^T[ACGT]AGG
AvaII        1104  GGACC                G^G[AT]CC
BalI         1619  TGGCCA               TGG^CCA
BbsI         1335  GTCTTC               GAAGAC(2/6)
BclI         1197  TGATCA               T^GATCA
BcnI           10  CCGGG                CC^[GC]GG
BmyI          889  GGGCAC               G[AGT]GC[ACT]^C
BpuAI        1335  GTCTTC               GAAGAC(2/6)
BsaJI        1093  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI        1010  ACCGGA               [AT]^CCGG[AT]
BsgI          497  CTGCAC               GTGCAG(16/14)
BsiYI         132  CCTTCAGAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          132  CCTTCAGAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI        1103  GGGAC                GGGAC(10/14)
BsmI          695  GCATTC               GAATGC(1/-1)
Bsp1286I      889  GGGCAC               G[AGT]GC[ACT]^C
BstAPI        199  GCAGAAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        869  GGTCACC              G^GT[ACGT]ACC
BstPI         869  GGTCACC              G^GT[ACGT]ACC
BstUI        1634  CGCG                 CG^CG
BstXI        1622  CCAGGCAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1549  CCTGAGG              CC^T[ACGT]AGG
CfoI         1633  GCGC                 GCG^C
DraII         191  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        872  CACCTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1619  TGGCCA               [CT]^GGCC[GA]
Eco81I       1549  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      191  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I       869  GGTCACC              G^GT[ACGT]ACC
EcoT14I      1093  CCAAGG               C^C[AT][AT]GG
EcoT22I       693  ATGCAT               ATGCA^T
FauI         1635  GCGGG                CCCGC(4/6)
FbaI         1197  TGATCA               T^GATCA
HhaI         1633  GCGC                 GCG^C
HinP1I       1633  GCGC                 G^CGC
MaeII         925  ACGT                 A^CGT
MluNI        1619  TGGCCA               TGG^CCA
MscI         1619  TGGCCA               TGG^CCA
MvnI         1634  CGCG                 CG^CG
NciI           10  CCGGG                CC^[GC]GG
NdeI         1258  CATATG               CA^TATG
NsiI          693  ATGCAT               ATGCA^T
NspI          508  GCATGT               [GA]CATG^[CT]
PstI          798  CTGCAG               CTGCA^G
SapI         1252  GAAGAGC              GCTCTTC(1/4)
SfcI          798  CTGCAG               C^T[GA][CT]AG
SspI         1157  AATATT               AAT^ATT
StyI         1093  CCAAGG               C^C[AT][AT]GG
XcmI          272  CCAAATTGCTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI         1374  AAATTC               [GA]^AATT[CT]
             1393  AAATTT              
AlwI         1412  GATCC                GGATC(4/5)
              916  GGATC               
ApoI         1374  AAATTC               [GA]^AATT[CT]
             1393  AAATTT              
Asp700I       127  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              831  GAATGACTTC          
BanI          554  GGCACC               G^G[CT][GA]CC
              902  GGCACC              
BglII         534  AGATCT               A^GATCT
              616  AGATCT              
BstF5I        516  GGATG                GGATG(2/0)
              768  GGATG               
BstNI         899  CCAGG                CC^[AT]GG
             1622  CCAGG               
Cac8I          14  GCAGGC               GC[ACGT]^[ACGT]GC
              156  GCAGGC              
Cfr13I        192  GGCCC                G^G[ACGT]CC
             1104  GGACC               
EcoRII        899  CCAGG                ^CC[AT]GG
             1622  CCAGG               
FokI          516  GGATG                GGATG(9/13)
              768  GGATG               
HapII          10  CCGG                 C^CGG
             1011  CCGG                
HpaII          10  CCGG                 C^CGG
             1011  CCGG                
HphI          577  GGTGA                GGTGA(8/7)
              871  TCACC               
MseI          562  TTAA                 T^TAA
             1640  TTAA                
MspI           10  CCGG                 C^CGG
             1011  CCGG                
MvaI          899  CCAGG                CC^[AT]GG
             1622  CCAGG               
PleI         1502  GACTC                GAGTC(4/5)
              852  GAGTC               
Sau96I        192  GGCCC                G^G[ACGT]CC
             1104  GGACC               
TaqI          749  TCGA                 T^CGA
              928  TCGA                
Tru9I         562  TTAA                 T^TAA
             1640  TTAA                
TthHB8I       749  TCGA                 T^CGA
              928  TCGA                
XmnI          127  GAAAGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              831  GAATGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BfaI        
BsaI        BsmAI       BsrI        BssKI       BstYI       
DdeI        DpnII       EarI        Fnu4HI      HaeIII      
HinfI       ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
ScrFI       SfaNI       TfiI        TseI        Tsp45I      
Tsp509I     TspRI       XhoII       
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