Restriction map for pF1KB8663
 (Linear) (Six-base) MAPSORT of: KIBB8663.seq  Check: 9455  from: 1  to: 1674



KIBB8663.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     488    1322    1674 
   Size:        118     370     834     352

  Fragments arranged by size: 

                 834     370     352     118
 
ApoI r'AATT_y

Cuts at:      0    1374    1393    1674 
   Size:       1374      19     281

  Fragments arranged by size: 

                1374     281      19
 
BanI G'GyrC_C

Cuts at:      0     554     902    1674 
   Size:        554     348     772

  Fragments arranged by size: 

                 772     554     348
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1328    1674 
   Size:       1328     346
 
BclI T'GATC_A

Cuts at:      0    1197    1674 
   Size:       1197     477
 
BfrBI ATG'CAT

Cuts at:      0     695    1674 
   Size:        695     979
 
BglII A'GATC_T

Cuts at:      0     534     616    1674 
   Size:        534      82    1058

  Fragments arranged by size: 

                1058     534      82
 
Bme1580I G_kGCm'C

Cuts at:      0     893    1674 
   Size:        893     781
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1205    1495    1674 
   Size:       1205     290     179

  Fragments arranged by size: 

                1205     290     179
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1482    1674 
   Size:       1482     192
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     106     814     971    1536    1674 
   Size:        106     708     157     565     138

  Fragments arranged by size: 

                 708     565     157     138     106
 
BsaWI w'CCGG_w

Cuts at:      0    1010    1674 
   Size:       1010     664
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482    1674 
   Size:        482    1192
 
BsmI GAATG_Cn'

Cuts at:      0     695    1674 
   Size:        695     979
 
Bsp1286I G_dGCh'C

Cuts at:      0     893    1674 
   Size:        893     781
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     205    1674 
   Size:        205    1469
 
BstEII G'GTnAC_C

Cuts at:      0     869    1674 
   Size:        869     805
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1629    1674 
   Size:       1629      45
 
BstYI r'GATC_y

Cuts at:      0     534     616    1411    1674 
   Size:        534      82     795     263

  Fragments arranged by size: 

                 795     534     263      82
 
Bsu36I CC'TnA_GG

Cuts at:      0    1550    1674 
   Size:       1550     124
 
BtsI GCAGTG_nn'

Cuts at:      0     768    1562    1674 
   Size:        768     794     112

  Fragments arranged by size: 

                 794     768     112
 
DraIII CAC_nnn'GTG

Cuts at:      0     877    1674 
   Size:        877     797
 
EaeI y'GGCC_r

Cuts at:      0    1619    1674 
   Size:       1619      55
 
EarI CTCTTCn'nnn_

Cuts at:      0     575    1063    1226    1247    1674 
   Size:        575     488     163      21     427

  Fragments arranged by size: 

                 575     488     427     163      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     488    1322    1674 
   Size:        118     370     834     352

  Fragments arranged by size: 

                 834     370     352     118
 
EcoO109I rG'GnC_Cy

Cuts at:      0     192    1674 
   Size:        192    1482
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     100     132    1674 
   Size:        100      32    1542

  Fragments arranged by size: 

                1542     100      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1439    1674 
   Size:       1439     235
 
MscI TGG'CCA

Cuts at:      0    1621    1674 
   Size:       1621      53
 
NdeI CA'TA_TG

Cuts at:      0    1259    1674 
   Size:       1259     415
 
NsiI A_TGCA'T

Cuts at:      0     697    1674 
   Size:        697     977
 
NspI r_CATG'y

Cuts at:      0     512    1674 
   Size:        512    1162
 
PstI C_TGCA'G

Cuts at:      0     802    1674 
   Size:        802     872
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1247    1674 
   Size:       1247     427
 
SfcI C'TryA_G

Cuts at:      0     798    1674 
   Size:        798     876
 
SspI AAT'ATT

Cuts at:      0    1159    1674 
   Size:       1159     515
 
StyI C'CwwG_G

Cuts at:      0    1093    1674 
   Size:       1093     581
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     857    1674 
   Size:        857     817
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     279    1674 
   Size:        279    1395
 
XmnI GAAnn'nnTTC

Cuts at:      0     131     835    1674 
   Size:        131     704     839

  Fragments arranged by size: 

                 839     704     131
 

 Enzymes that do cut:

     AcuI     ApoI     BanI     BbsI     BclI    BfrBI    BglII Bme1580I
     BmrI     BplI     BsaI    BsaWI     BsgI     BsmI Bsp1286I   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtsI   DraIII     EaeI     EarI
  Eco57MI EcoO109I    Hin4I     MmeI     MscI     NdeI     NsiI     NspI
     PstI     SapI     SfcI     SspI     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BglI     BlpI    BmgBI     BmtI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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