Restriction map for pF1KB8662
##################################
# NO CUT ENZYMES (  148 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BfaI        BfrI        BglI        
BlnI        BpuAI       BsaAI       BsaHI       BsaI        
BsgI        BsiEI       BsiWI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        KasI        KpnI        KspI        MaeI        
MaeIII      MluI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StyI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII         370  CGCG                 CG^CG
AccIII        926  TCCGGA               T^CCGGA
AcsI          410  AAATTC               [GA]^AATT[CT]
AfaI          310  GTAC                 GT^AC
ApoI          410  AAATTC               [GA]^AATT[CT]
AspHI          94  GAGCTC               G[AT]GC[AT]^C
BalI          497  TGGCCA               TGG^CCA
BanI          951  GGCACC               G^G[CT][GA]CC
BcgI          431  CGAGAGAAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI           96  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       96  GCTCAGC              GC^T[ACGT]AGC
BsaBI         245  GATCCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         926  TCCGGA               [AT]^CCGG[AT]
BseAI         926  TCCGGA               T^CCGGA
BsiHKAI        94  GAGCTC               G[AT]GC[AT]^C
BspEI         926  TCCGGA               T^CCGGA
BsrDI         799  GCAATG               GCAATG(2/0)
BsrI          159  ACTGG                ACTGG(1/-1)
BssSI         385  CACGAG               CACGAG(-5/-1)
BstAPI        214  GCAGATTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         370  CGCG                 CG^CG
CelII          96  GCTCAGC              GC^T[ACGT]AGC
DraII         644  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          270  CCGTGG               C^C[GA][CT]GG
EcoO109I      644  AGGCCCT              [GA]G^G[ACGT]CC[CT]
FauI           80  GCGGG                CCCGC(4/6)
HgaI           23  GCGTC                GACGC(5/10)
HindIII       787  AAGCTT               A^AGCTT
MamI          245  GATCCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         497  TGGCCA               TGG^CCA
MroI          926  TCCGGA               T^CCGGA
MscI          497  TGGCCA               TGG^CCA
MslI         1011  CATCAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          370  CGCG                 CG^CG
PshAI         966  GACTTACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          310  GTAC                 GT^AC
SacI           94  GAGCTC               GAGCT^C
SapI         1005  GAAGAGC              GCTCTTC(1/4)
SspI          146  AATATT               AAT^ATT
StuI          279  AGGCCT               AGG^CCT
TspRI         911  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI          245  GATCC                GGATC(4/5)
              171  GGATC               
BanII          94  GAGCTC               G[GA]GC[CT]^C
              163  GAGCCC              
BglII         830  AGATCT               A^GATCT
              983  AGATCT              
BsmAI         127  GAGAC                GTCTC(1/5)
              700  GAGAC               
BspMI         374  ACCTGC               ACCTGC(4/8)
              762  GCAGGT              
BstF5I        336  GGATG                GGATG(2/0)
             1016  GGATG               
BstNI         167  CCAGG                CC^[AT]GG
              999  CCTGG               
EaeI          497  TGGCCA               [CT]^GGCC[GA]
              938  TGGCCG              
EarI          631  GAAGAG               CTCTTC(1/4)
             1005  GAAGAG              
EcoRII        167  CCAGG                ^CC[AT]GG
              999  CCTGG               
FokI          336  GGATG                GGATG(9/13)
             1016  GGATG               
HphI          636  GGTGA                GGTGA(8/7)
              960  GGTGA               
Ksp632I       631  GAAGAG               CTCTTC(1/4)
             1005  GAAGAG              
MaeII         567  ACGT                 A^CGT
              971  ACGT                
MfeI          616  CAATTG               C^AATTG
              704  CAATTG              
MspA1I         73  CAGCTG               C[AC]G^C[GT]G
              934  CAGCTG              
MunI          616  CAATTG               C^AATTG
              704  CAATTG              
MvaI          167  CCAGG                CC^[AT]GG
              999  CCTGG               
PleI           34  GACTC                GAGTC(4/5)
               13  GAGTC               
PstI          376  CTGCAG               CTGCA^G
              760  CTGCAG              
PvuII          73  CAGCTG               CAG^CTG
              934  CAGCTG              
SfaNI         381  GCATC                GCATC(5/9)
             1010  GCATC               
SfcI          376  CTGCAG               C^T[GA][CT]AG
              760  CTGCAG              
SmlI          301  CTTGAG               C^T[CT][GA]AG
              697  CTTGAG              
TfiI          717  GAATC                G^A[AT]TC
             1077  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AluI        AvaII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BssKI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       Fnu4HI      HaeIII      
HapII       HinfI       HpaII       ItaI        MboI        
MboII       MflI        MnlI        MseI        MspI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       TaqI        Tru9I       
TseI        Tsp509I     TthHB8I     XhoII       
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